Sri Sai Priya Avuthu

Motivated biotechnology graduate student with hands-on experience in molecular biology, cell culture, and cancer research. Skilled in designing experiments, optimizing protocols and contributing to translational research in molecular biology and biotechnology. Proficient in techniques such as ELISA, PCR, Western blot, and microscopy, with a proven ability to analyze data and deliver impactful results. Passionate about applying scientific expertise to drive innovations in biotechnology and therapeutic development.

Homology of Unknown Protein Using Swiss-Prot - KL University, Vijayawada, India  Oct 2022 – Dec 2022

• Performed homology searches using Swiss-Prot and BLAST, identifying proteins with over 90% sequence similarity & matching functional annotations providing insights into potential conserved functions, evolutionary relationships

• Analyzed potential therapeutic applications of identified proteins, examining their roles in disease pathways, proposing candidates for targeted drug delivery systems, and evaluating suitability for mRNA-based therapies

Amplification of Human Genomic DNA via Touchdown PCR - KL University, Vijayawada, India    Jul 2022 – Sep 2022

• Streamlined Touchdown PCR to enhance primer binding specificity, boosting DNA amplification efficiency by 40%

• Validated amplified DNA through agarose gel electrophoresis, ensuring reproducibility across 50+ samples for downstream molecular applications

Dissociation studies of Immune complexes using plant extracts - KL University, Vijayawada, India  Aug 2021 - Jan 2022

• Investigated the dissociation of immune complexes using plant extracts through ELISA assays, focusing on alternatives to chemical substances for safer therapeutic applications.
• Screened ten plant leaf extracts, identifying six with promising dissociation properties, offering potential for natural and effective solutions in immune complex clearance.

Detection and characterization of resistant genes in Tomato seeds - KL University, Vijayawada, India  Jan 2021 - June 2021

• Analyzed genetic purity and pest resistance in hybrid tomato seeds (PHS165, HYVEG, and CYNUS varieties) using TD-PCR and FTIR studies to ensure consistency and quality.
• Evaluated post-harvest loss solutions, focusing on resistant gene detection and characterization to enhance the durability and pest resistance of commercial tomato hybrids.

• Detection and Characterization of Resistant Gene in Tomato Seeds of PHS165, Hyveg, and Cynus Hybrid Varieties, 09/01/2023, http://dx.doi.org/10.22377/ajp.v17i03.4984
• Exploring the Molecular Mechanisms of Angiogenesis in Cancer, 06/01/2023, http://dx.doi.org/10.21474/IJAR01/17048
• Touchdown PCR for Increased Sensitivity and Specificity in Amplification of Mouse Cognate Homeobox, 08/22/2022, https://www.journalijar.com/article/42076/touchdown-pcr-for-increased-sensitivity-and-specificity-in-amplification-of-mouse-cognate-homeobox/

• Vice President, Aarambh Productions – Graduate Student Club, Northeastern University
  Oversee all club operations, showcasing leadership and organizational skills while fostering my passion for content creation. Successfully coordinated cultural and professional events, ensuring seamless execution and high member engagement.
• President, BioTrendz – Undergraduate Department Club, KL University
  Organized events such as poster presentations, national-level tech fests, webinars, and seminars, bringing people together and enhancing departmental engagement. Spearheaded initiatives to collaborate with industry professionals, providing students with valuable learning and networking opportunities.

• Base SAS, Cavaxia - 04/2021
• Clinical Database Management System, Cavaxia - 03/2021
• Regulatory Affairs, Cavaxia - 01/ 2021
• Research methodology: Statistical tools for data analysis and interpretation, Mizoram University - 09/2020