Subhajit Roy
Irvine,CA
Summary
Successful academic researcher with 7+ years of experience in active laboratory research. Currently a third year PhD research student enrolled at University of California, Irvine with MS degree and substantial research background, publications, patents and awards. Looking for industrial internship positions for a post-PhD transition into industrial scientist career. Hardworking, responsible, patient and a flexible team-player with an attitude of continuous self study, wise decision-making and good communication skill.
Overview
6
6
years of professional experience
Work History
PhD Student
University of California, Irvine
Irvine, CA
08.2022 - Current
- Conducting toxicological research based on a wide variety of cell culture based and animal models with knockout mice, humanized NSG mice and development of robust animal models for different toxicological exposure (PB, Permethrin, Microcystins, Cylindrospermopsin) and infectious disease co-exposure models of non-cholera Vibrios, climate change animal models of periodic heat stress.
- Exposure models using primary cells and different cell lines, generating knockout and knock-in stable cells.
- Research methodology by using various techniques i.e. fluorescent and confocal microscopy, western blotting, qRT-PCR, Co-IP, ChIP, microbiome (WMS), FACS, ELISA and other immunological assays.
- Drafting research manuscripts.
- Animal Protocol writing and prepared submissions for Institutional Review Board or Ethics Committee approval, amendments, renewals.
- Data analyses.
- Instructed students individually or in groups to reinforce learning concepts.
Graduate Research Assistant
University of South Carolina
Columbia, SC
01.2022 - 07.2022
- Animal model development of toxicologic exposures.
- Data generation using various molecular tools and techniques.
- Animal protocol writing and IACUC logistics.
- Drafting Manuscript based on generated data.
- Investigated gene composition and expression, as well as genetic engineering principles and effects.
UGC-Senior Research Fellowship
National Institute Of Biomedical Genomics
Kolkata, West Bengal, India
01.2019 - 12.2021
- Clinical research, Patient sample handling.
- Genotyping (KASP based and TaqMan based) serum profiling (AlphaLisa, ELISA), immune cell isolations from human subjects.
- Cell based assays, cell isolation using MACS and FACS.
- Functional Genomic validations.
- R-studio (different packages) for data generation and statistical analyses.
Junior Research Fellowship (Welcome-DBT Funded)
Institute of Life Sciences (ILS)
Bhubaneshwar, Odisha, India
05.2018 - 12.2019
- Studied Autophagy and innate immune response using IRGM null mice.
- Experimental Tissue preparation, blocking and immunohistochemical analyses.
- Worked with multiple cell lines and stable cell generation.
- Report writing.
Summer Internship
Indian Association for Cultivation of Sciences
Kolkata, West Bengal, India
01.2018 - 04.2018
- Drug screening on cell lines and through biochemical assays (Topoisomerase inhibitors, DNA damage responses, DNA cleavage assay, EMSA etc.).
- FRAP based confocal studies on candidate drug with reference to camptothecin (CPT).
- Assistance in patent filling, also achieved a patent on one of the candidate drugs, research published at JMC)
Education
Ph.D. - Environmental Toxicology
University of California, Irvine
Irvine, CA06-2026
Master of Science - Zoology And Molecular Biology
University of Burdwan
Burdwan, West Bengal, India08-2017
Bachelor of Science - Zoology, Chemistry And Human Physiology
Hooghly Mohsin College
Chinsurah, West Bengal, India07-2015
Skills
- Development of Animal models (Toxicochemical exposures and non-cholera Vibrio infections, climate change models), oral gavaging, IP administer of toxins, cardiac puncture, serum collection, organ collection, tissue preparation, primary cell isolation from liver and intestine etc
- Cell culture-based techniques: Adherent, suspension cell cultures, transfection, knockdown and FACS based staining protocols, MACS for T cell, Macrophages and DC isolation
- Molecular Techniques: Isolation of DNA, RNA, Protein isolation, Gel based separation (Agarose, SDS-Page, Native), Cloning, PCR, qRT-PCR, Western Blot
- Valedictory techniques: Immunofluorescence, Immunohistochemitry, EMSA, IP/co-IP, ChIP, alpha-LISA (PerkinElmer), ELISA etc
- Microscopy: Regular user of Fluorescence Microscope for IF, live cell imaging and FRAP for Confocal based drug screening
- Genomic Techniques: Genotyping (KASP based and TaqMan based), Magnetic bead-based library preparation for NGS platform
- In-silico based and dry-lab techniques: R studio (packages)- processed data (count matrix), bar and dot plots, volcano lots, violin plots, correlation matrix etc and UCSF Chimera for limited molecular docking work
Publications, Patent and Awards
PUBLICATIONS
1. Functional genetic variants of the IFN-λ3 (IL28B) gene and transcription factor interactions on its promoter. Roy S, Guha Roy D, Bhushan A, Bharatiya S, Chinnaswamy S. Cytokine. 2021 Jun;142:155491. doi: 10.1016/j.cyto.2021.155491. Epub 2021 Mar 13. PMID: 33725487; PMCID: PMC7611124.
2. Periodic Heat Wave-induced neuronal etiology in the elderly is mediated by the Gut-Liver-Brain axis: A Transcriptome Profiling Approach. Roy S, Saha P, Bose D, Trivedi A, More M, Christina Lin, Jenny Wu, Melanie Oakes, and Saurabh Chatterjee. (Communicated to Nature Scientific Reports, December 2023)
3. NLRP3-Derived Hsp70 Binding to TLR4 Mediates MASLD to MASH Progression upon Inhibition of PP2A by Harmful Algal Bloom Toxin Microcystin, a Second Hit. Roy S, Saha P, Bose D, Trivedi A, More M, Xiao S, Diehl AM, Chatterjee S. Hepatic Int J Mol Sci. 2023 Nov 15;24(22):16354. doi: 10.3390/ijms242216354. PMID: 38003543; PMCID: PMC10671242.
4. Subhajit Roy, Punnag Saha, Dipro Bose, Saurabh Chatterjee; Host Gut-Resistome and Gut-Immune Axes are the key to Non-cholera Vibrioinfection severity in aging and heat stress. J Immunol 1 May 2023; 210 (1_Supplement): 81.02. https://doi.org/10.4049/jimmunol.210.Supp.81.02
5. Distinct molecular phenotypes involving several human diseases are induced by ifn- λ3 and ifn-λ4 in monocyte-derived macrophages. De, M., Bhushan, A., Grubbe, W.S., Roy S., Mendoza J.L., et al. Genes Immun 23, 73–84 (2022). https://doi.org/10.1038/s41435-022-00164-w
6. Trivedi, A.; Bose, D.; Saha, P.; Roy, S.; More, M.; Skupsky, J.; Klimas, N.G.; Chatterjee, S. Prolonged Antibiotic Use in a Preclinical Model of Gulf War Chronic Multisymptom-Illness Causes Renal Fibrosis-like Pathology via Increased micro-RNA 21-Induced PTEN Inhibition That Is Correlated with Low Host Lachnospiraceae Abundance. Cells 2024, 13, 56. https://doi.org/10.3390/cells13010056
7. Subchronic Oral Cylindrospermopsin Exposure Alters the Host Gut Microbiome and Is Associated with Progressive Hepatic Inflammation, Stellate Cell Activation, and Mild Fibrosis in a Preclinical Study Saha, P.; Upright, M.; Bose, D.; Roy, S.; Trivedi, A.; More, M.; Scott, G.I.; Brooks, B.W.; Chatterjee, S.. Toxins 2022, 14, x.https://doi.org/10.3390/xxxxx
8. More, M., Saha, P., Bose, D., Trivedi, A., Roy, S., Chatterjee, S et al. Host microbiome associated low intestinal acetate correlates with progressive NLRP3-dependent hepatic-immunotoxicity in early life microcystin-LR exposure. BMC Pharmacol Toxicol 24, 78 (2023). https://doi.org/10.1186/s40360-023- 00721-7
9. Bose D, Stebliankin V, Cickovski T, Saha P, Trivedi A, Roy S, More M, Tuteja A, Mathee K, Narasimhan G, Chatterjee S. Microbiome Dysbiosis Shows Strong Association of Gut-Derived Altered Metabolomic Profile in Gulf War Chronic Multisymptom Illness Symptom Persistence Following Western Diet Feeding and Development of Obesity. Int J Mol Sci. 2023 Feb 20;24(4):4245. doi: 10.3390/ijms24044245. PMID: 36835663; PMCID: PMC9962797.
PATENT
Bicycle Topoisomerase I Inhibiting Compounds, Process for Preparation and Use Thereof.
Publication Number WO/2019/229765 Publication Date 05.12.2019 International Application No. PCT/IN2019/050410 International Filing Date 24.05.2019 Applicants
Council Of Scientific & Industrial Research, India • Indian Association for The Cultivation of Science, India
Inventors: Talukdar, Arindam; Das, Benu Brata; Kundu, Biswajit; Das, Subhendu K; Paul, Chowdhuri Srijita; Sarkar, Dipayan; Pal, Sourav; Bhattacharya, Debomita; Mukherjee, Ayan; Roy, Subhajit.
AWARDS/HONORS
· AAI Immunology, Best Abstract and Podium Presentation Award, 2023
· SOT ASIO Best Poster Award, 2023
· UCI DEOH Travel Award, 2023
· CSIR/UGC-NET JRF (2017) June 2017, AIR: 45
· IIT- GATE (Life Sciences), 2018, AIR: 307
· BHU MS Entrance, 2015, AIR: 17
· IIT-JAM- 2015, AIR: 44
References
References available upon request.
Timeline
PhD Student
University of California, Irvine
08.2022 - Current
Graduate Research Assistant
University of South Carolina
01.2022 - 07.2022
UGC-Senior Research Fellowship
National Institute Of Biomedical Genomics
01.2019 - 12.2021
Junior Research Fellowship (Welcome-DBT Funded)
Institute of Life Sciences (ILS)
05.2018 - 12.2019
Summer Internship
Indian Association for Cultivation of Sciences
01.2018 - 04.2018
Ph.D. - Environmental Toxicology
University of California, Irvine
Master of Science - Zoology And Molecular Biology
University of Burdwan
Bachelor of Science - Zoology, Chemistry And Human Physiology
Hooghly Mohsin College
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