Susan Shumaker
Collierville,TN
Summary
Organized and dependable candidate successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals.
Overview
23
23
years of professional experience
Work History
Medical Technologist III – Molecular Pathology-Clinical Genomics Laboratory
St. Jude Children's Hospital
08.2017 - Current
- Whole, Exome, RNA Sequencing: Library Prep –(WGS) Whole Genomic Sequencing, (WES) Whole Exome Sequencing, and (RNASeq) RNA Sequencing
- Sequencing on Illumina Nova Seq 6000
- Sequencing on Illumina NextSeq 2000
- Sequencing on Illumina MiSeq
- Extraction techniques and processes: Cell lysis of Bone Marrow and Peripheral blood with Cell count determinations RNA extraction via Qiagen RNeasy column (Manual)
- Hamilton Robot Library Prep
- RNA extraction via Kingfisher Flex robot (Automation, High Throughput) DNA extraction via Chemagen Robot (Automation)
- DNA quantification: Nano drop -Spectrophotometer
- Qubit 3.0 Fluorometer
- Agilent 4200 Tape Station
- Invitrogen E-Gel
- Real Time PCR: RNA extraction with Qiagen Kit for Minimal Residual Disease (MRD) testing of suspected ALL, AML, MLL, and Solid Tumor patients Panel testing with designated Transcripts for Cancer on the ABI 7500 using Bio-Rad and Vilo Master mix kits in conjunction with home-brew assays.
- Infinium HD Methylation Array: Screening of over 850,000 methylation sites with Copy Number Determination (CNV) on the Epic Bead Chip Microarray for diagnosis and classification of central nervous system tumors on Research and Clinical samples.
- Bone Marrow Transplant Engraftment Analysis for Chimerism testing: DNA extraction on Chimagen Robot system with downstream fragment analysis on the ABI 3500 followed by manual calculations to determine donor/patient engraftment percentage.
- Covid Testing: KingFisher extraction of patient specimens suspected of Covid-19 infection using RealStar SARS-CoV-2-RT-PCR kit for qPCR testing with analysis on ABI 3500.
- Factor II and V Leiden testing: Roche LightCycler 2.0 analysis of extracted DNA product from Chimagen Robot systems.
- Super User for Epic Beaker LIMS: Super User, responsible for design, training, and implementation of the Epic Beaker LIMS system for slated go-live in October 2022.
- Centron Temperature Monitoring: On call rotation for Centron automated temperature monitoring of all Freezers and Refrigerators (approximately 25 total) in two distinct campus locations.
- Pipet Calibration: Responsible for managing calibration, maintenance and repairs of 175 manual Pipets on a recurring 6 month schedule Repairs performed via 3rd party send-out.
Medical Technologist – (LC/MS Pain Management)
Compass Laboratory Services
04.2013 - 08.2017
- Operated and maintained 6 Agilent LC/MS instruments for Pain Management Provided testing for both Parent and Metabolites of 100+ prescription medications in urine specimens Analyzed and released all calibrators, controls, and patient sample results as a part of QA/QC.
Medical Technologist – (Clinical Chemistry)
Compass Laboratory Services
04.2013 - 08.2017
- Reviewed for accuracy and released all sample data from the Roche Cobas Called critical and alert values to clients Handled all send-out results for data entry to the lab system for release.
Medical Technologist – (Nuclear Magnetic Resonance)
Compass Laboratory Services
04.2013 - 08.2017
- NMR testing for Cholesterol particles such as sLDL, HDLp, and LDL Prep of Calibrators, controls, and patient samples Check QC and Data for accuracy and release batches.
Medical Technologist - (Flow Cytometry)
Poplar Healthcare
01.2013 - 04.2013
- Blood workup in Flow Cytometry in processing bone marrow for comprehensive screen workups and antibody setups for cancer diagnosis Performed inventory and ordering of supplies for the lab
- Entering results for initial evaluation and release to clients.
Medical Technologist – (Immunology and Organ Donor workups for Midsouth Transplant and Arkansas Regional Organ Recovery Agency)
Interstate Blood Bank
01.2012 - 01.2013
- Test for HIV, HCV, HBs through screening with Abbot Prism; Confirmation with RIBA, Western Blot, HIV-2 Syphilis PRP screen, and ABO and Rh testing by manual and Immucor Galileo testing.
Medical Technologist – (Toxicology and Special Chemistry)
American Esoteric Laboratories
01.2011 - 01.2012
- Multilevel extraction process of urine in preparation for GC/MS analysis Analyzed and determined patient results from the GC/MS data
- Report results for positive and negative confirmations
- Special chemistry testing: Tacrolimus, Cyclosporine, Citrate, Oxalate, ACER, ICal, and PBB.
Medical Technologist – (Drug Screening)
Advanced Toxicology Network (Concentra)
01.2005 - 01.2011
- Immunoassay screening on Abbott Aeroset instruments Data paperwork checked and signed for all calibrations and controls, certifies accurate entry of screening results
- All samples reviewed for Invalid and Substituted specimens.
Medical Technologist – (Immunology and Flow Cytometry)
University of Tennessee
01.2003 - 01.2005
- Flow cytometry cross match of Organ donor and recipient for organ transplantation by Mid-South Transplant CMV quantification on the ABI 7700.
Medical Technologist – (Blood Bank)
Baptist Memorial Hospital
01.2001 - 01.2003
- Type and cross match, blood, and antibody identification Provided storage for stem cells.
Education
MS - Master of Business in Healthcare Management
Arkansas State University
05.2024
BS - Medical Technologist – Allied Health
University of Tennessee Health Science Center
05.2001
BS - Radio & Television Production – Mass Communications
Middle Tennessee State University
05.1997
Skills
- Specimen Analysis
- Specimen Processing
- Equipment Testing and Calibration
- Quality Control
- Laboratory Equipment Maintenance
- Inventory Control
- High Complexity Testing
- Regulatory Compliance
- Specimen Testing and Analysis
- Detail Oriented
- Data Entry
Timeline
Medical Technologist III – Molecular Pathology-Clinical Genomics Laboratory
St. Jude Children's Hospital
08.2017 - Current
Medical Technologist – (LC/MS Pain Management)
Compass Laboratory Services
04.2013 - 08.2017
Medical Technologist – (Clinical Chemistry)
Compass Laboratory Services
04.2013 - 08.2017
Medical Technologist – (Nuclear Magnetic Resonance)
Compass Laboratory Services
04.2013 - 08.2017
Medical Technologist - (Flow Cytometry)
Poplar Healthcare
01.2013 - 04.2013
Medical Technologist – (Immunology and Organ Donor workups for Midsouth Transplant and Arkansas Regional Organ Recovery Agency)
Interstate Blood Bank
01.2012 - 01.2013
Medical Technologist – (Toxicology and Special Chemistry)
American Esoteric Laboratories
01.2011 - 01.2012
Medical Technologist – (Drug Screening)
Advanced Toxicology Network (Concentra)
01.2005 - 01.2011
Medical Technologist – (Immunology and Flow Cytometry)
University of Tennessee
01.2003 - 01.2005
Medical Technologist – (Blood Bank)
Baptist Memorial Hospital
01.2001 - 01.2003
MS - Master of Business in Healthcare Management
Arkansas State University
BS - Medical Technologist – Allied Health
University of Tennessee Health Science Center
BS - Radio & Television Production – Mass Communications
Middle Tennessee State University
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