Tam Nguyen
San Francisco,CA
Summary
Experienced professional seeks engaging lab environment to showcase collaborative and critical thinking skills, driving biotech industry growth through excellent communication, analytical, and lab expertise.
Overview
5
5
years of professional experience
Work History
Research Associate
Encoded Therapeutics
08.2023 - Current
- Managed routine passaging of 293/T17 cells using 2-layer and 5-layer cell stacks, ensuring optimal cell health for downstream processes
- Conducted transfection experiments, optimizing conditions for efficient viral vector production with phelper, rep capsid, and GOI
- Coordinated cell stack harvesting post-transfection at the critical 50-hour time point, employing systematic procedures for timely collection
- Utilized advanced purification techniques, including chromatography and ultracentrifugation, to isolate and concentrate viral vectors with high yields and purity
- Performed comprehensive quality control analyses on purified samples using alkaline gel electrophoresis to assess vector integrity and purity
- Recorded experimental protocols, observations, and results in Benchling LIMS software, ensuring accurate documentation and traceability
- Collaborated with cross-functional teams to troubleshoot issues, optimize protocols, and drive continuous improvement in vector production processes.
Associate Scientist
Allogene Therapeutics
11.2021 - 04.2023
- Performed Quantitative PCR and ddPCR to identify the occurrence of the viral genes with genomic DNA in the PBMCs/Leukopak, in-process sample from CART process, and drug product
- Supported the viral vector team with the integrated provirus sequence investigation by optimizing different PCR conditions/protocol and analyzing multiple E-gel images using 2 PCR (SuperFi and Takara) kits in comparison to solve the LTI reported presence of a truncated provirus
- Cultured and maintained T cell lines and primary cell for the downstream operation
- Expanded cell culture, cryopreserved cells for future testing of HHV6 and HHV7
- Whole Cell based assay running ddPCR determining the ratio of live and dead cells
- Assisted cross functional groups with HHV testing when results are needed for multiple samples such as large scale samples
- Currently learning Flow Cytometry
- Experienced with inputting data into Benchling
- Maintained good lab practice preventing samples from contamination.
Associate/Senior Formulation Technician
Thermo Fisher Scientific
05.2019 - 11.2021
- Collaborated with support teams (Manufacturing Sciences) in optimizing C/D methods for specific oligo sequences to aid in increased first-pass yield, removing/impurities
- Supported development of various protocols for UNICORN software used with AKTA synthesizers
- Ensuring proper product specifications are met
- Developed and implemented new method for changing out adapters for AKTA synthesizers
- Leading to reduction in synthesis start time and instrument downtime due to pinched reagent lines
- Monitored instrument calibration and Preventative Maintenance schedules, ensuring the lab is able to forecast instrument availability and maintain flexibility to meet delivery metrics
- Assist management in creating and tracking Problem Records within the lab as necessary to adhere to site Quality Assurance requirements
- Work alongside Quality Assurance support team to ensure all Manufacturing necessary Processing Documents are complete and allow product release to customer, in a timely manner
- Actively communicated with internal and external vendors to address manufacturing related issues
- Assisted with onboarding of new operators and helped ensure important information was passed down to manufacturing team.
Education
Bachelor of Science: Human Biology -
University of California, Merced
Merced, CA12.2018
Skills
- Collaborative
- Critical Thinking
- Communication
- Analytical
- Transfection Experiments
- Viral Vector Production
- Cell Stack Harvesting
- Viral Vector Isolation
- Viral Vector Concentration
- Quality Control
- Alkaline Gel Electrophoresis
- Vector Integrity Assessment
- Benchling LIMS Software
- Documentation and Traceability
- Troubleshooting and Protocol Optimization
- Continuous Improvement
- Quantitative PCR and ddPCR
- Viral Gene Identification
- Primary Cell Culture Techniques (PBMCs, Leukopak, Genomic DNA)
- Whole Cell-Based Assay Development
Timeline
Research Associate
Encoded Therapeutics
08.2023 - Current
Associate Scientist
Allogene Therapeutics
11.2021 - 04.2023
Associate/Senior Formulation Technician
Thermo Fisher Scientific
05.2019 - 11.2021
Bachelor of Science: Human Biology -
University of California, Merced
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