Tammy Cavallo
Cary,NC
Summary
Experienced scientific professional specializing in molecular biology, mammalian cell culture, and vector production. Skilled in project management and compliant with GMP, GLP, and GDP standards. Proven ability to conduct research and perform detailed data and statistical analysis. Dedicated to advancing research through meticulous work, strong organizational skills, and effective collaboration.
Overview
12
12
years of professional experience
1
1
Certification
Work History
Vector Production Scientist
UNC Chapel Hill, School of Medicine, Lineberger Comprehensive Cancer Center
Chapel Hill, NC
08.2020 - Current
- Conducted experimental assays to support cancer research initiatives.
- Developed and optimized protocols for sample preparation and analysis.
- Implemented quality control measures to ensure data integrity and reliability.
- Trained and mentored junior staff in laboratory techniques and safety practices.
- Managed laboratory inventory, sourcing materials to maintain operational continuity.
- Maintained a safe work environment, adhering to all safety protocols and guidelines.
- Contributed to continuous improvement efforts by suggesting and implementing process enhancements.
- Coordinated project timelines, ensuring alignment with research objectives and milestones.
- Project Management
- Document Management
- Mammalian Cell Culture
- DNA/RNA Extraction and Quantification
- Immunofluorescence Staining
- Flow Cytometry
- Production of Retroviral Producing Master Cell Bank
- Production of Retroviral Supernatant with iCellis Nano Bioreactor
- Vector Copy Number Assay Analysis/Reporting
- Transgene Expression Analysis/Reporting
- Replication Competent Retrovirus (RCR) Assay Analysis/Reporting
- QPCR and ddPCR Data Acquisition, Analysis, and Report Generation
- GMP/GLP/GDP
- Viral Transfection/Transduction
Scientist
Icagen
Durham, NC
12.2017 - 08.2020
- DNA Transfection: X-tremeGene HP (Sigma) for Cloning Workflow
- Gene Transfer and Expression: cells, promoter, envelope, pantropic expression system for Cloning Workflow
- Bacterial Ligation/Transformation: XL-10 Gold Ultracompetent Cells (Agilent) for Cloning Workflow
- Inoculate Petri Dishes at Different Concentrations with LB broth to Generate Single Cell Clones
- Isolate and Quantify DNA/RNA
- Synthesize cDNA for PCR amplification
- Performed Enzymatic Digest of DNA/Plasmid for fragment isolation
- Gel Electrophoresis – Nucleic Acid Electrophoresis
- Analyzing Gel Electrophoresis Data, Generate Report
- DNA extraction and purification from agarose gel
- Analysis of DNA sequencing data using DNAStar to confirm Open Reading Frame (ORF)
- Tissue Culture for numerous HEK, CHO, Fibroblast, HBE, FRT cell lines
- Transwell plates: air-liquid phase cell culture to develop tight junctions.
Research Associate II
Humacyte
Morrisville, NC
01.2017 - 11.2017
- Mammalian Cell Culture - extensive mammalian cell culture experience, passage, proliferation, cryopreservation.
- Human Tissue Isolation - Isolate primary human heart artery cells to culture, passage, cryopreserve and/or perform research & development cell-based assays including collagen production assay
- RNA Isolation - isolate RNA to perform RT-qPCR analysis to characterize cells
- Immunofluorescence Staining - stain cell surface markers with antibodies to characterize cells via flow cytometry analysis.
- Compound/Prepare Reagents for use in mammalian cell culture and cell-based assays.
Biologist
RTI International
Research Triangle Park, NC
10.2014 - 01.2017
- Performed Metabolomics Research to identify metabolites most important in separating two groups, disease vs. non-disease
- Those important metabolites were traced in biochemical mapping software (GeneGo) to obtain pathway information to potentially discover biomarkers for drug development
- Mammalian Tissue Culture, Passage and Cryopreservation for multiple cell lines: Caco-2, HT29-MTX, Calu-3, Raw 264.7, PBMCs, dakiki
- Immunofluorescence Staining Flow Cytometry Antibody Titration for Flow Cytometry
- Cytokine Antibody Array
- CytoViva Darkfield Microscopy with Hyperspectral Imaging Technology
- Assemble and Maintain Microfluidic Devices
- Solid Phase Extraction (SPE)
- GC/TOF and GC/MS (Gas Chromatography/Time of Flight and Gas Chromatography/Mass Spectrometry) Chromatogram Processing and Identification
- LC-MS Training NMR Spectra Processing and Identification
- GeneGo Pathway Mapping Analysis
- Multivariate Statistical Data Analysis (SIMCA Software)
- Submitted Proposal for Research Idea and Received Funding for Internal Flow Cytomtetry Meets Metabolomics Research Project
- Project Management
- Budget Preparation and Management
- Poster Presentations
- RTI Eastern Regional Comprehensive Metabolomics Resource Core
Intern
RTI International
Research Triangle Park, NC
06.2014 - 10.2014
- Culture and passage cell lines: Caco-2, HT29-MTX and Raw 264.7
- CytoViva Darkfield Microscopy Training
- NMR Spectroscopy Training
- Extraction/Dissolution Sample Preparation
- Flow Cytometry Training
- Poster Presentations
Education
Bachelor of Science - Biological Sciences, Human Biology
North Carolina State University
Raleigh, NCSkills
- Molecular Biology Techniques/Methods
- Good manufacturing practice compliance
- Project Management
- Quality assurance
- Quantitative analysis
- Document Management
- Experimental method design
- Assay development/optimization
- Report Preparation/Presentation
- Failure Analysis
- Planning/Coordination/Scheduling
- Continuous improvement
Certification
- Project Management Professional - PMP Certification
- Lean Six Sigma Yellow Belt
Accomplishments
- “Microfluidics meets metabolomics to reveal the impact of Campylobacter jejuni infection on biochemical pathways”, Mortensen N.P., Mercier K.A., McRitchie S., Cavallo T.B., Pathmasiri W., Stewart D., Sumner S.J., , Biomedical Microdevices Journal, June 2016, online date: May 2016, DOI10.1007/ s10544-016-0076-9, Print ISSN1387-2176, Online ISSN1572
- “CD30-Directed CAR-T Cells Co-Expressing CCR4 in Relapsed/Refractory Hodgkin Lymphoma and CD30+ Cutaneous T Cell Lymphoma”, Natalie S Grover 1, Anastasia Ivanova 1, Dominic T. Moore 1, Catherine Joyce Arago Cheng 1, Caroline Babinec 1, John West 1, Tammy Cavallo 1, J. Kaitlin Morrison 1, Faith Brianne Buchanan 1, Edith Bowers 2, Christopher Dittus 1, Anne W. Beaven 1, Gianpietro Dotti 1, Jonathan S. Serody 1, Barbara Savoldo 1; https://doi.org/10.1182/blood-2021-148102
- “Safety and Efficacy of CD30.CAR-T Cells as Consolidation Post Autologous Transplant in High-Risk CD30 Lymphoma” Grover, Natalie and Hucks, George and Riches, Marcie and Ivanova, Anastasia and Moore, Dominic and Shea, Thomas and Seegars, Mary Beth and Armistead, Paul and Kasow, Kimberly and Beaven, Anne and Dittus, Christopher and Coghill, James and Jamieson, Katarzyna and Vincent, Benjamin and Wood, William and Cheng, Catherine and Morrison, J. Kaitlin and West, John and Cavallo, Tammy and Dotti, Gianpietro and Serody, Jonathan and Savoldo, Barbara, Safety and Efficacy of CD30.CAR-T Cells as Consolidation Post Autologous Transplant in High-Risk CD30 Lymphoma. Available at SSRN: https://ssrn.com/abstract=4689900 or http://dx.doi.org/10.2139/ssrn.4689900
- “Clinical Activity of Anti-PD-1 Therapy Following CD30 CAR-T Cell Therapy in Relapsed Hodgkin Lymphoma” Timothy J. Voorhees, Anne W. Beaven, Christopher Dittus, George E Hucks, J. Kaitlin Morrison, Catherine Joyce Arago Cheng, Tammy Cavallo, Steven I. Park, Gianpietro Dotti, Jonathan S. Serody, Barbara Savoldo, Natalie S. Grover Blood (2022) 140 (Supplement 1): 12723–12724. https://doi.org/10.1182/blood-2022-156660
Timeline
Vector Production Scientist
UNC Chapel Hill, School of Medicine, Lineberger Comprehensive Cancer Center
08.2020 - Current
Scientist
Icagen
12.2017 - 08.2020
Research Associate II
Humacyte
01.2017 - 11.2017
Biologist
RTI International
10.2014 - 01.2017
Intern
RTI International
06.2014 - 10.2014
Bachelor of Science - Biological Sciences, Human Biology
North Carolina State University