Summary
Overview
Work History
Education
Skills
References
Publications
Timeline
Generic

THOMAS KLUZ

Monroe,NY

Summary

Quality focused Senior Research Technician with 30 years of experience working in Dr. Max Costa's Lab studying molecular mechanisms of metal Carcinogenesis, Cancer and Chromatin structure. Well organized and attentive to details. Able to work independently and as a team member.

Overview

32
32
years of professional experience

Work History

Senior Research Technician / Dr. Costa Lab

NYU Grossman School Of Medicine Dept. Of Medicine
01.2000 - Current
  • Extraction of RNA, DNA, and proteins from various samples
  • Mammalian cell transfection (siRNA and plasmid DNA)
  • Soft Agar Assay (colony formation assay)
  • Gene Expression analysis by RT-qPCR
  • Cytotoxicity assays
  • RNA and DNA Library preparation for Next Generation Sequencing (Dr. Costa lab and Epigenomics Core)
  • Western blotting and ELISA assays
  • Acid digestion of biological and non-biological samples for ICP-MS analysis
  • ICP-MS Analysis of Trace Elements and Heavy Metals
  • Method development and measurement of Taurine in human serum samples using HPLC
  • Gene specific methylation analysis using methylation sensitive digestion enzymes or methylation-specific PCR
  • Assist in training new lab members and students
  • Maintain and repair lab equipment
  • Manage lab supplies inventory, track orders
  • Maintain chemical inventory, adhere to all safety protocols and best practices in the laboratory
  • Stay current with new developments in the field.

Lab Technician / Dr. Costa Lab

NYU Department Of Environmental Medicine
01.1994 - 01.2000
  • PCR
  • DNA cloning
  • DNA-protein crosslinks analysis
  • Gene expression analysis by Northern blot and RT-PCR
  • Maintain cell cultures
  • DNA/RNA isolation and sequencing
  • Affymetrix GeneChip library preparation.

Lab Assistant

NYU Department Of Environmental Medicine
07.1992 - 01.1994
  • Perform all basic housekeeping duties, prepare laboratory samples and reagents according to established protocols.

Education

Sixty Science Course Credits -

Sunny Westchester And Sunny Rockland Community College

Skills

  • Cell culture
  • RNA, DNA, Protein isolation from TC cells and tissues
  • PCR, qPCR, RT-qPCR
  • Southern, Western and Northern blotting techniques
  • Elisa assays
  • DNA methylation analysis
  • RNA-seq
  • CHIP-seq
  • Micro RNA-Seq
  • HPLC
  • Immunoprecipitation
  • ICP-MS analysis - method development, sample digestion and analysis using Perkin Elmer NexIon 350D ICP-MS instrument

References

Will be provided upon request.

Publications

1. Zhang Z, Kluz T, Costa M. Toxic element contaminations of prenatal vitamins. Toxicol Appl Pharmacol. 2023 Oct 15;477:116670. doi: 10.1016/j.taap.2023.116670. Epub 2023 Aug 29. PMID: 37652310.


2. Ortiz A, Sun H, Kluz T, Matsui MS, Carle T, Gan D, Gordon T, Gildea L, Costa M. A combination of three antioxidants decreases the impact of rural particulate pollution in Normal human keratinocytes. Int J Cosmet Sci. 2023 Aug 21. doi: 10.1111/ics.12888. Epub ahead of print. PMID: 37602524.


3. Park SH, Lu Y, Shao Y, Prophete C, Horton L, Sisco M, Lee HW, Kluz T, Sun H, Costa M, Zelikoff J, Chen LC, Gorr MW, Wold LE, Cohen MD. Longitudinal Impact of WTC Dust Inhalation on Rat Cardiac Tissue Transcriptomic Profiles. Int J Environ Res Public Health. 2022 Jan 14;19(2):919. doi: 10.3390/ijerph19020919. PMID: 35055737; PMCID: PMC8776213.


4. Park SH, Lu Y, Shao Y, Prophete C, Horton L, Sisco M, Lee HW, Kluz T, Sun H, Costa M, Zelikoff J, Chen LC, Cohen MD. Longitudinal impact on rat cardiac tissue transcriptomic profiles due to acute intratracheal inhalation exposures to isoflurane. PLoS One. 2021 Oct 14;16(10):e0257241. doi: 10.1371/journal.pone.0257241. PMID: 34648499; PMCIDPMC8516213.


5. Chen D, Chen QY, Wang Z, Zhu Y, Kluz T, Tan W, Li J, Wu F, Fang L, Zhang X, He R, Shen S, Sun H, Zang C, Jin C, Costa M. Polyadenylation of Histone H3.1 mRNA Promotes Cell Transformation by Displacing H3.3 from Gene Regulatory Elements. iScience. 2020 Sep 1;23(9):101518. doi: 10.1016/j.isci.2020.101518. PMID: 32920490; PMCID: PMC7492993


6. Chen QY, Shen S, Sun H, Wu F, Kluz T, Kibriya MG, Chen Y, Ahsan H, Costa M. PBMC gene expression profiles of female Bangladeshi adults chronically exposed to arsenic-contaminated drinking water. Environ Pollut. 2020 Apr;259:113672. doi: 10.1016/j.envpol.2019.113672. Epub 2019 Dec 26. PMID: 31918125.


7. Chen QY, Li J, Sun H, Wu F, Zhu Y, Kluz T, Jordan A, DesMarais T, Zhang X, Murphy A, Costa M. Role of miR-31 and SATB2 in arsenic-induced malignant BEAS-2B cell transformation. Mol Carcinog. 2018 Aug;57(8):968-977. doi: 10.1002/mc.22817. Epub 2018 Apr 17. PMID: 29603397; PMCID: PMC6588163.


8. Marais TLD, Kluz T, Xu D, Zhang X, Gesumaria L, Matsui MS, Costa M, Sun H. Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation. Sci Rep. 2017 Oct 19;7(1):13622. doi: 10.1038/s41598-017-13765-7. PMID: 29051608; PMCID: PMC5648893.


9. Laulicht-Glick F, Wu F, Zhang X, Jordan A, Brocato J, Kluz T, Sun H, Costa M. Tungsten exposure causes a selective loss of histone demethylase protein. Mol Carcinog. 2017 Jul;56(7):1778-1788. doi: 10.1002/mc.22634. Epub 2017 Mar 30. PMID: 28218462; PMCID: PMC5708117.


10. Chen D, Kluz T, Fang L, Zhang X, Sun H, Jin C, Costa M. Hexavalent Chromium (Cr(VI)) Down-Regulates Acetylation of Histone H4 at Lysine 16 through Induction of Stressor Protein Nupr1. PLoS One. 2016 Jun 10;11(6):e0157317. doi: 10.1371/journal.pone.0157317. PMID: 27285315; PMCID: PMC4902237.


11. Cartularo L, Kluz T, Cohen L, Shen SS, Costa M. Molecular Mechanisms of Malignant Transformation by Low Dose Cadmium in Normal Human Bronchial Epithelial Cells. PLoS One. 2016 May 17;11(5):e0155002. doi: 10.1371/journal.pone.0155002. PMID: 27186882; PMCID: PMC4871351.


12. Zhang X, Kluz T, Gesumaria L, Matsui MS, Costa M, Sun H. Solar Simulated Ultraviolet Radiation Induces Global Histone Hypoacetylation in Human Keratinocytes. PLoS One. 2016 Feb 26;11(2):e0150175. doi: 10.1371/journal.pone.0150175. PMID: 26918332; PMCID: PMC4769140.


13. Wu F, Jordan A, Kluz T, Shen S, Sun H, Cartularo LA, Costa M. SATB2 expression increased anchorage-independent growth and cell migration in human bronchial epithelial cells. Toxicol Appl Pharmacol. 2016 Feb 15;293:30-6. doi: 10.1016/j.taap.2016.01.008. Epub 2016 Jan 11. PMID: 26780400; PMCID: PMC4748731.


14. Brocato J, Hernandez M, Laulicht F, Sun H, Shamy M, Alghamdi MA, Khoder MI, Kluz T, Chen LC, Costa M. In Vivo Exposures to Particulate Matter Collected from Saudi Arabia or Nickel Chloride Display Similar Dysregulation of Metabolic Syndrome Genes. J Toxicol Environ Health A. 2015;78(23-24):1421-36. doi: 10.1080/15287394.2015.1095689. PMID: 26692068; PMCID: PMC4709028.


15. Jagannathan L, Jose CC, Arita A, Kluz T, Sun H, Zhang X, Yao Y, Kartashov AV, Barski A, Costa M, Cuddapah S. Nuclear Factor κB1/RelA Mediates Inflammation in Human Lung Epithelial Cells at Atmospheric Oxygen Levels. J Cell Physiol. 2016 Jul;231(7):1611-20. doi: 10.1002/jcp.25262. Epub 2015 Dec 10. PMID: 26588041; PMCID: PMC4845657.


16. Cartularo L, Laulicht F, Sun H, Kluz T, Freedman JH, Costa M. Gene expression and pathway analysis of human hepatocellular carcinoma cells treated with cadmium. Toxicol Appl Pharmacol. 2015 Nov 1;288(3):399-408. doi: 10.1016/j.taap.2015.08.011. Epub 2015 Aug 24. PMID: 26314618; PMCID: PMC4605876.


17.Yao Y, Chen T, Shen SS, Niu Y, DesMarais TL, Linn R, Saunders E, Fan Z, Lioy P, Kluz T, Chen LC, Wu Z, Costa M, Zelikoff J. Malignant human cell transformation of Marcellus Shale gas drilling flow back water. Toxicol Appl Pharmacol. 2015 Oct 1;288(1):121-30. doi: 10.1016/j.taap.2015.07.011. Epub 2015 Jul 22. Erratum in: Toxicol Appl Pharmacol. 2016 Apr 1;296():85. Zelikoff, Judith [Added]. PMID: 26210350; PMCID: PMC4698968.


18. Laulicht F, Brocato J, Cartularo L, Vaughan J, Wu F, Kluz T, Sun H, Oksuz BA, Shen S, Peana M, Medici S, Zoroddu MA, Costa M. Tungsten-induced carcinogenesis in human bronchial epithelial cells. Toxicol Appl Pharmacol. 2015 Oct 1;288(1):33-9. doi: 10.1016/j.taap.2015.07.003. Epub 2015 Jul 9. PMID: 26164860; PMCID: PMC4579035.


19. Muñoz A, Chervona Y, Hall M, Kluz T, Gamble MV, Costa M. Sex-specific patterns and deregulation of endocrine pathways in the gene expression profiles of Bangladeshi adults exposed to arsenic contaminated drinking water. Toxicol Appl Pharmacol. 2015 May 1;284(3):330-8. doi: 10.1016/j.taap.2015.02.025. Epub 2015 Mar 7. PMID: 25759245; PMCID: PMC4410068.


20. Gesumaria L, Matsui MS, Kluz T, Costa M. Solar-simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts. Exp Dermatol. 2015 May;24(5):384-5. doi: 10.1111/exd.12675. Epub 2015 Mar 9. PMID: 25707437; PMCID: PMC4471858.


21. Brocato J, Sun H, Shamy M, Kluz T, Alghamdi MA, Khoder MI, Chen LC, Costa M. Particulate matter from Saudi Arabia induces genes involved in inflammation, metabolic syndrome and atherosclerosis. J Toxicol Environ Health A. 2014;77(13):751-66. doi: 10.1080/15287394.2014.892446. PMID: 24839929; PMCID: PMC4233653.


22. Arita A, Muñoz A, Chervona Y, Niu J, Qu Q, Zhao N, Ruan Y, Kiok K, Kluz T, Sun H, Clancy HA, Shamy M, Costa M. Gene expression profiles in peripheral blood mononuclear cells of Chinese nickel refinery workers with high exposures to nickel and control subjects. Cancer Epidemiol Biomarkers Prev. 2013 Feb;22(2):261-9. doi: 10.1158/1055-9965.EPI-12-1011. Epub 2012 Nov 29. PMID: 23195993; PMCID: PMC3565097.


23. Sun H, Shamy M, Kluz T, Muñoz AB, Zhong M, Laulicht F, Alghamdi MA, Khoder MI, Chen LC, Costa M. Gene expression profiling and pathway analysis of human bronchial epithelial cells exposed to airborne particulate matter collected from Saudi Arabia. Toxicol Appl Pharmacol. 2012 Dec 1;265(2):147-57. doi: 10.1016/j.taap.2012.10.008. Epub 2012 Oct 17. PMID: 23085030; PMCID: PMC3883356.


24. Clancy HA, Sun H, Passantino L, Kluz T, Muñoz A, Zavadil J, Costa M. Gene expression changes in human lung cells exposed to arsenic, chromium, nickel or vanadium indicate the first steps in cancer. Metallomics. 2012 Aug;4(8):784-93. doi: 10.1039/c2mt20074k. Epub 2012 Jun 19. PMID: 22714537; PMCID: PMC3563094.


25. Wu F, Sun H, Kluz T, Clancy HA, Kiok K, Costa M. Epigallocatechin-3-gallate (EGCG) protects against chromate-induced toxicity in vitro. Toxicol Appl Pharmacol. 2012 Jan 15;258(2):166-75. doi: 10.1016/j.taap.2011.10.018. Epub 2011 Nov 4. PMID: 22079256; PMCID: PMC3259276.


26. Kiok K, Sun H, Clancy H, Bose S, Kluz T, Wu F, Costa M. Liprin-α4 is required for nickel induced receptor protein tyrosine phosphatase-leukocyte antigen related receptor F (RPTP-LAR) activity. PLoS One. 2011;6(8):e22764. doi: 10.1371/journal.pone.0022764. Epub 2011 Aug 4. PMID: 21829649; PMCID: PMC3150438.


27. Tchou-Wong KM, Kiok K, Tang Z, Kluz T, Arita A, Smith PR, Brown S, Costa M. Effects of nickel treatment on H3K4 trimethylation and gene expression. PLoS One. 2011 Mar 24;6(3):e17728. doi: 10.1371/journal.pone.0017728. PMID: 21455298; PMCID: PMC3063782.


28. Sun H, Clancy HA, Kluz T, Zavadil J, Costa M. Comparison of gene expression profiles in chromate transformed BEAS-2B cells. PLoS One. 2011 Mar 18;6(3):e17982. doi: 10.1371/journal.pone.0017982. PMID: 21437242; PMCID: PMC3060877.


29. Chen H, Kluz T, Zhang R, Costa M. Hypoxia and nickel inhibit histone demethylase JMJD1A and repress Spry2 expression in human bronchial epithelial BEAS-2B cells. Carcinogenesis. 2010 Dec;31(12):2136-44. doi: 10.1093/carcin/bgq197. Epub 2010 Sep 29. PMID: 20881000; PMCID: PMC2994281.


30. Zhou X, Sun H, Chen H, Zavadil J, Kluz T, Arita A, Costa M. Hypoxia induces trimethylated H3 lysine 4 by inhibition of JARID1A demethylase. Cancer Res. 2010 May 15;70(10):4214-21. doi: 10.1158/0008-5472.CAN-09-2942. Epub 2010 Apr 20. PMID: 20406991; PMCID: PMC3007597.


31. Li Q, Kluz T, Sun H, Costa M. Mechanisms of c-myc degradation by nickel compounds and hypoxia. PLoS One. 2009 Dec 31;4(12):e8531. doi: 10.1371/journal.pone.0008531. PMID: 20046830; PMCID: PMC2797325.


32. Ellen TP, Kluz T, Harder ME, Xiong J, Costa M. Heterochromatinization as a potential mechanism of nickel-induced carcinogenesis. Biochemistry. 2009 Jun 2;48(21):4626-32. doi: 10.1021/bi900246h. PMID: 19338343; PMCID: PMC2713359.


33. Uddin AN, Burns FJ, Rossman TG, Chen H, Kluz T, Costa M. Dietary chromium and nickel enhance UV-carcinogenesis in skin of hairless mice. Toxicol Appl Pharmacol. 2007 Jun 15;221(3):329-38. doi: 10.1016/j.taap.2007.03.030. Epub 2007 Apr 14. PMID: 17499830.


34. Ke Q, Davidson T, Kluz T, Oller A, Costa M. Fluorescent tracking of nickel ions in human cultured cells. Toxicol Appl Pharmacol. 2007 Feb 15;219(1):18-23. doi: 10.1016/j.taap.2006.08.013. Epub 2006 Oct 28. PMID: 17239912.


35. Ke Q, Kluz T, Costa M. Down-regulation of the expression of the FIH-1 and ARD-1 genes at the transcriptional level by nickel and cobalt in the human lung adenocarcinoma A549 cell line. Int J Environ Res Public Health. 2005 Apr;2(1):10-3. doi: 10.3390/ijerph2005010010. PMID: 16705796; PMCID: PMC3814691.


36. Chen H, Ke Q, Kluz T, Yan Y, Costa M. Nickel ions increase histone H3 lysine 9 dimethylation and induce transgene silencing. Mol Cell Biol. 2006 May;26(10):3728-37. doi: 10.1128/MCB.26.10.3728-3737.2006. PMID: 16648469; PMCID: PMC1488989.


37. Ke Q, Davidson T, Chen H, Kluz T, Costa M. Alterations of histone modifications and transgene silencing by nickel chloride. Carcinogenesis. 2006 Jul;27(7):1481-8. doi: 10.1093/carcin/bgl004. Epub 2006 Mar 7. PMID: 16522665.


38. Costa M, Davidson TL, Chen H, Ke Q, Zhang P, Yan Y, Huang C, Kluz T. Nickel carcinogenesis: epigenetics and hypoxia signaling. Mutat Res. 2005 Dec 30;592(1-2):79-88. doi: 10.1016/j.mrfmmm.2005.06.008. Epub 2005 Jul 11. PMID: 16009382.


39. Davidson T, Kluz T, Burns F, Rossman T, Zhang Q, Uddin A, Nadas A, Costa M. Exposure to chromium (VI) in the drinking water increases susceptibility to UV-induced skin tumors in hairless mice. Toxicol Appl Pharmacol. 2004 May 1;196(3):431-7. doi: 10.1016/j.taap.2004.01.006. PMID: 15094314.


40. Zhao J, Chen H, Davidson T, Kluz T, Zhang Q, Costa M. Nickel-induced 1,4-alpha-glucan branching enzyme 1 up-regulation via the hypoxic signaling pathway. Toxicol Appl Pharmacol. 2004 May 1;196(3):404-9. doi: 10.1016/j.taap.2004.01.012. PMID: 15094311.


41. Zhao J, Yan Y, Salnikow K, Kluz T, Costa M. Nickel-induced down-regulation of serpin by hypoxic signaling. Toxicol Appl Pharmacol. 2004 Jan 1;194(1):60-8. doi: 10.1016/j.taap.2003.08.011. PMID: 14728980.


42. Zhang Q, Salnikow K, Kluz T, Chen LC, Su WC, Costa M. Inhibition and reversal of nickel-induced transformation by the histone deacetylase inhibitor trichostatin A. Toxicol Appl Pharmacol. 2003 Nov 1;192(3):201-11. doi: 10.1016/s0041-008x(03)00280-1. PMID: 14575637.


43. Yan Y, Kluz T, Zhang P, Chen HB, Costa M. Analysis of specific lysine histone H3 and H4 acetylation and methylation status in clones of cells with a gene silenced by nickel exposure. Toxicol Appl Pharmacol. 2003 Aug 1;190(3):272-7. doi: 10.1016/s0041-008x(03)00169-8. PMID: 12902198.


44. Salnikow K, Davidson T, Kluz T, Chen H, Zhou D, Costa M. GeneChip analysis of signaling pathways effected by nickel. J Environ Monit. 2003 Apr;5(2):206-9. doi: 10.1039/b210262p. PMID: 12729255.


45. Costa M, Salnikow K, Sutherland JE, Broday L, Peng W, Zhang Q, Kluz T. The role of oxidative stress in nickel and chromate genotoxicity. Mol Cell Biochem. 2002 May-Jun;234-235(1-2):265-75. PMID: 12162442.


46. Zhang Q, Kluz T, Salnikow K, Costa M. Comparison of the cytotoxicity, cellular uptake, and DNA-protein crosslinks induced by potassium chromate in lymphoblast cell lines derived from three different individuals. Biol Trace Elem Res. 2002 Apr;86(1):11-22. doi: 10.1385/BTER:86:1:11. PMID: 12002656.


47. Salnikow K, Kluz T, Costa M, Piquemal D, Demidenko ZN, Xie K, Blagosklonny MV. The regulation of hypoxic genes by calcium involves c-Jun/AP-1, which cooperates with hypoxia-inducible factor 1 in response to hypoxia. Mol Cell Biol. 2002 Mar;22(6):1734-41. doi: 10.1128/MCB.22.6.1734-1741.2002. PMID: 11865053; PMCID: PMC135615.


48. Costa M, Sutherland JE, Peng W, Salnikow K, Broday L, Kluz T. Molecular biology of nickel carcinogenesis. Mol Cell Biochem. 2001 Jun;222(1-2):205-11. PMID: 11678603.


49. Sutherland JE, Zhitkovich A, Kluz T, Costa M. Rats retain chromium in tissues following chronic ingestion of drinking water containing hexavalent chromium. Biol Trace Elem Res. 2000 Apr;74(1):41-53. doi: 10.1385/BTER:74:1:41. PMID: 11049199.


50. Salnikow K, Kluz T, Costa M. Role of Ca(2+) in the regulation of nickel-inducible Cap43 gene expression. Toxicol Appl Pharmacol. 1999 Oct 15;160(2):127-32. doi: 10.1006/taap.1999.8759. PMID: 10527911.


51. Zhitkovich A, Voitkun V, Kluz T, Costa M. Utilization of DNA-protein cross-links as a biomarker of chromium exposure. Environ Health Perspect. 1998 Aug;106 Suppl 4(Suppl 4):969-74. doi: 10.1289/ehp.98106s4969. PMID: 9703480; PMCID: PMC1533346.

Timeline

Senior Research Technician / Dr. Costa Lab

NYU Grossman School Of Medicine Dept. Of Medicine
01.2000 - Current

Lab Technician / Dr. Costa Lab

NYU Department Of Environmental Medicine
01.1994 - 01.2000

Lab Assistant

NYU Department Of Environmental Medicine
07.1992 - 01.1994

Sixty Science Course Credits -

Sunny Westchester And Sunny Rockland Community College

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