Timothy Barrette
Sterling,MA
Summary
ASCP certified medical technologist and technical supervisor with substantial experience in clinical laboratory operations and diagnostics. Adept at performing highly complex laboratory tests, maintaining lab equipment, performing new employee and annual training and competency, and ensuring accuracy in test results. Strong emphasis on team collaboration, inter-departmental communication, and adaptability in dynamic environments, with focus on achieving accurate patient results. Skilled in using advanced laboratory technology and adhering to stringent safety and quality standards.
Overview
27
27
years of professional experience
1
1
Certification
Work History
Medical Technologist III, Technical Supervisor Genetics, ASCP(MB)
Boston Heart Diagnostics (a division of Eurofins Scientific)
Framingham, MA
08.2013 - Current
- Oversee genetics department as the technical supervisor
- Maintain daily and monthly genetics departmental paperwork
- Perform real-time PCR genetic testing by allelic discrimination (SNP ASO testing) utilizing QuantStudio software
- Characterize HDL sub-particles utilizing electrophoresis, Western blotting, I125 antibody labelling, and proprietary image analysis
- Quantify patient CoQ10 enzyme concentrations by UPLC utilizing Waters/Acquity analysis software
- Build, track, and release sample testing through LIMS software
- Maintain current certification as a Medical Technologist in Molecular Biology (CLSpMB, ASCP)
- Oversee competency compliance requirements for laboratory personnel across multiple testing platforms and technical groups
- Involved with stability testing for extension of assay controls
- Oversee and perform assay validations and testing changes, write associated SOPs
- Maintain inventory, source new genetics assay controls, and validate new reagent lots
- Lead training and mentorship of new Medical Technologists
Molecular Technologist II
Laboratory Corporation of America
Westborough, MA
05.2004 - 07.2013
- Performed Cystic Fibrosis and Ashkenazi Jewish mutation screening utilizing ASO dot-blot techniques and radioactive analysis (P32)
- Screened for Fragile X disease utilizing both Southern blot and PCR techniques
- Sequenced and reported subclonal populations for diagnosis of Chronic Lymphocytic Leukemia (CLL)
- Became Certified Lab Specialist in Molecular Biology through ASCP (CLSpMB, ASCP)
- Tested for maternal cell contamination (PCR), methylation, and Pompe mutation disease (Western blot)
- Collected and labeled samples for development of a new FRAX testing platform (PCR based)
- Became familiar with 5S and LEAN techniques, trained as first responder and member of problem solving group
- Utilized LIMS system for patient data tracking and release
- Initiated and completed assay change controls/validations
- Trained new hires within the group and completed competency assessments
- Participated in ongoing education in the Transition to Leadership series
Research Associate II and Study Director, Molecular Biology
BioReliance
Worcester, MA
02.2001 - 04.2004
- Progressed from Laboratory Assistant to Research Associate II to Study Director in two years
- Extracted nucleic acid (DNA, RNA) and performed real time Polymerase Chain Reaction (Q-PCR and RT Q-PCR) using Applied Biosystems equipment
- Carried out viral load safety testing for clients in the biopharmaceutical industry under GLP/GMP guidelines
- Performed cell line characterization; mycoplasma and virus detection
- Reviewed, interpreted data, and wrote final reports for submission to US and European regulatory authorities
- Wrote SOPs, deviation and investigation reports, and amendment documentation to GLP/GMP standards
- Assisted in protocol development, assay qualification, and technical transfers to ICH guidelines
- Interacted interdepartmentally with QA, QC and project management teams to accomplish testing and ensure quality and compliance
- Trained as a radiation handler (H3), first aid officer and member of the safety committee
- Organized and implemented inventory tracking systems
Research Assistant
Beth Israel Deaconess Medical Center
Boston, MA
06.1999 - 09.2000
- Supported principal investigation into novel diabetes research
- Isolated cardiomyocytes from transgenic mice and set up subsequent cell culture
- Extracted DNA, RNA and protein from muscle tissues
- Performed Northern and Western blotting
- Prepared reagents
- ELISA testing
Lab Assistant
Oral Roberts University
Tulsa, OK
09.1999 - 05.2000
- Sectioned, stained and prepared slides for histology laboratory
- Cared for research animals in the biology department
Education
Bachelor of Science - Pre-med Biology
Oral Roberts University
Tulsa05-2000
Skills
- Single nucleotide polymorphism (SNP) genetic genotyping
- New employee training and competency documentation
- LIMS
- UPLC testing (Waters/Acquity software)
- Data review, assay validations, report, and SOP writing
- Assay change control and validation
- GLP/GMP guideline familiarity
- Interdepartmental communication and technical report writing
- ABI 3100, 3730, 3730 XL and 7700, and TaqMan analysis
- DNA, RNA, protein and cell extraction
- Electrophoresis (agarose, SDS PAGE)
- Isoenzyme analysis and DNA fingerprinting
- ASO dot-blotting
- DNA sequencing
- Reagent preparation, plasmid purification
- Animal handling
- 5S and LEAN organizational skills
- PCR, Q-PCR and RT Q-PCR technique
- F-PERT and RT assays
- Southern, Northern and Western blotting
- ELISA
- Spectrophotometry
- Certified radiation handling (H3, P32, I125)
- Slide preparation/histology
Certification
- CLSpMB, ASCP - Certified Laboratory Specialist, Molecular Biology
Timeline
Medical Technologist III, Technical Supervisor Genetics, ASCP(MB)
Boston Heart Diagnostics (a division of Eurofins Scientific)
08.2013 - Current
Molecular Technologist II
Laboratory Corporation of America
05.2004 - 07.2013
Research Associate II and Study Director, Molecular Biology
BioReliance
02.2001 - 04.2004
Lab Assistant
Oral Roberts University
09.1999 - 05.2000
Research Assistant
Beth Israel Deaconess Medical Center
06.1999 - 09.2000
Bachelor of Science - Pre-med Biology
Oral Roberts University