Tirtha Lamichhane

Accomplished Postdoctoral Research Associate at the University of Florida, skilled in CRISPR/Cas genome editing and teamwork. Successfully led multidisciplinary research projects, enhancing gene delivery methods and advancing transgenic genome editing techniques. Proven ability to drive innovative solutions in molecular biology, contributing to impactful scientific discoveries.

• Annual Grad student symposium, University of Miami, FL, 2015, Poster Presentation on ‘Optical mapping method for the improvement of pea aphid genome assembly’
• American Phytopathological Society meeting, Cleveland, Ohio, 2019, Poster presentation on ‘Improvement of CRISPR/Cas9 genome editing efficacy in citrus’
• American Phytopathological Society meeting, Virtual meeting, 2021, Poster presentation on ‘Mechanistic understanding of Xcc-facilitated agroinfiltration to improve the efficacy of transient gene expression in citrus leaves’
• American Phytopathological Society meeting, Pittsburgh, Pennsylvania, 2022, Poster presentation on ‘Mechanistic understanding of Xcc-facilitated agroinfiltration to improve the efficacy of transient gene expression in citrus leaves’
• American Phytopathological Society meeting, Denver, Colorado, 2023, Poster presentation on ‘Optimization of agroinfiltration in citrus for transgene expression and genome editing’
• Society for In Vitro Biology (SIVB) meeting, Norfolk, Virginia, 2025, Poster and lightening talk presentation on ‘Exploration of the Interactions Between Xanthomonas citri subsp. citri-Agrobacterium-Citrus to Improve Agrobacterium-mediated Transient Expression in Plants’

• Strong skills in basic molecular skills PCR, DNA and RNA isolation, RT-qPCR, gene cloning, Western blot, RNAi
• Skills in CRISPR/Cas genome editing, gRNAs design and testing of gRNAs by in-vitro and in-planta assay, transgenic and non-transgenic genome editing approach
• Skills in transient and stable gene expression, tissue culture, gene delivery methods into plant cells by gene gun, polyethylene glycol (PEG), and Agrobacterium-mediated method
• Excellent teamwork and research skills demonstrated in conducting multidisciplinary research projects

• Recognition of reviewer certificate, Elsevier, The editors of STAR Protocols, Cell press open access journal, 2024
• Appreciation Certificate, Rotaract Club of Dillibazar, Kathmandu, Nepal, 2009
• Trichandra Youth Red-cross annual magazine, 2008

• Organized annual science exhibition, 2013, RAI school, Kathmandu, Nepal, Guiding students for poster presentation and demonstration of fundamental science experiments (photosynthesis, respiration, osmosis) to teach basic science to the visitors
• Service at Rotaract Club, 2009, Dillibazar, Nepal, Assist in health camp organization and management in health care checkup program of community schools’ children
• Youth Red Cross service, 2008, Tri-Chandra campus, Kathmandu, Nepal, Assist in blood donation program at Tri-Chandra Campus. Publication of Red Cross annual magazine

• American Phytopathological Society (APS), 01/01/19, 12/31/23
• Sigma Xi, The Scientific Research Honor Society, 01/01/25, current

Citrus Research and Education Center, Institute of Food and Agricultural Sciences (IFAS), University of Florida

863-956-8829

Title: Postdoctoral Research Associate

• Li J, Kolbasov V, Pang Z, Duan S, Lee D, Huang Y, Xu J, Teper D, Lamichhane T and Wang N, Evaluation of the control effect of SAR inducers against citrus Huanglongbing applied by foliar spray, soil drench or trunk injection, Phytopathol Res, 3, 2, 2021, 10.1186/s42483-020-00079-2
• Lamichhane, T., Su, H., Huang, X., & Wang, N., Exploration of the Interactions Between Xanthomonas citri subsp. citri-Agrobacterium-Citrus to Improve Agrobacterium-Mediated Transient Expression in Plants, Molecular plant-microbe interactions: MPMI, 2025, 10.1094/MPMI-12-24-0164-R
• Xiaoen Huang, Wenxiu Ma, Wenting Wang, Yu Feng, Tirtha Lamichhane, Jin Xu, Pandey Shankar Sheo, Diann S Achor, Jinyun Li, Yuanchun Wang, Javier L Dalmendray, Zhuyuan Hu, Camila Ribeiro, Ning Zhang, Madhurababu Kunta, Allison K Hansen, Nian Wang, Mechanistic dissection of Candidatus Liberibacter Triggered Chronic Immune Disease, https://doi.org/10.1101/2025.05.21.654963, preprint

Symbiosis Fest, 2015, University of Miami, Organized and communicated to the visitors about the symbiosis fest provided some food related to symbiosis and explained the poster related to symbiosis research Work-shop for high school students, 2024, University of Florida, The main activities involved the DNA extraction and gram staining methods

• Software carpentry python workshop, 2015, University of Miami, FL, USA
• Journalism training, 2006, Government of Nepal, Kathmandu, Nepal

RNA guide genome editing in citrus cells using CRISPR-ribonucleoprotein complexes and improved crispr/cas9 genome editing efficacy in citrus, N. Wang, X. Huang, T. Lamichhane, A. Parsaeimehr, S. Hang, S. Wang, 17/632,371, 2022

• Postdoctoral Research Associate, University of Florida, 01/01/23, present
• Graduate research assistant, University of Florida, 01/01/17, 12/31/23
• OPS, University of Florida, 01/01/16, 12/31/17