Tobias Johnston
Creative Biochemical Specialist
Sherman Oaks,CA
Summary
- I aspire to innovate through integrity and teamwork to create shared value for my employer, employees, community, and environment.
- Detail-oriented team player with strong organizational skills. Ability to handle multiple projects simultaneously with a high degree of accuracy.
Overview
16
16
years of professional experience
8
8
years of post-secondary education
Work History
Sr. Associate Process Development
Amgen Inc
07.2022 - 03.2024
- Works with process development teams across the country to develop strategies to efficiently and safety purify biological drugs in large scale
- Organize and direct manufacturing practices for small teams
- Operate protein chromatography and filtration machinery
- Write detailed reports of outcomes of isolation trials
- New Product Introduction Team Leader
- Ensured upstream bioreactor cell viability, DO, CO2, and media parameters were maintained until cell harvest
- Performed protein concentration assays including western blot, various UV-VIS techniques, and HPLC
- Analyzed chromatographs to ensure proper protein isolation/ separation
- Prioritized tasks to meet tight deadlines, pitching in to assist others with project duties
Research Fellow
Washington State University
05.2020 - 05.2022
- Conducted comprehensive literature reviews to identify knowledge gaps and inform future studies.
- Optimized experimental designs using advanced statistical techniques to ensure valid conclusions were drawn from collected data.
- Performed in-vivo experimentation on hippocampi including but not limited to: Semlicki Forest Viral Vector administration, simulating stroke through surgical intervention, confocal microscopy imaging of hippocampi
- Performed in-vitro studies on rat hippocampal slices, and primary neuronal cell lines
- Conducted protein analysis including but not limited to: Western blot, ELISA, chromatography & mass spec
- Performed other cellular molecular tactics including PCR, qPCR, cloning, and viral vector creation
- Determined bio-markers to indicate ischemia/reperfusion injury and recovery
Research Specialist
San Diego State Research Foundation
05.2018 - 06.2020
- Conduct cellular and molecular research for cardiac development and metabolic process
- Culturing and maintaining human/ mammalian cells
- Assisted in writing scientific papers including editing, and ensuring papers followed journal guidelines
- Performing perfusion of hearts on mice adn rats
- Performed in-vivo cardiac surgeries (Transaortic Restriction)
- Assisted and oversaw with pharmacological experiments
- Isolating cardiac myocytes from genetically altered mice and rats
- Genotyping, purifying, and isolating DNA along with characterization via western blot, ELISA, or HPLC
- Used well-known and characterized biomarkers to drive scientific decisions
- Uphold position of Lab Safety Officer
- Managed and organize animal colony
- Viable project determination/ management
- General lab management; organizing, and implementing protocols to ensure lab member participation of cleaning, organizing data, and accountability
- Analyzed variations in complex tests, experiments and procedures to produce research data.
- Guided technical programs and administrative activities in research settings and integrated quality assurance and safety programs.
- Implemented modifications and quality control measures to influence research conclusions.
- Modified experimental methods based upon interpretation of data.
- Communicated outcomes and results of research to sponsors.
Intern/fellow
National, Institute of Aging @ NIH
06.2017 - 08.2017
- Developed own in vivo study on genetic variant mice, and rats
- Analyzed problems and worked with teams to develop solutions.
- Completed research, compiled data, updated spreadsheets, and produced timely reports.
- Performed protein characterization and identification
- Basic Organic chemistry to alter compound structures for multipurpose drug interactions
- Confocal microscopy for visual representation of chemical activity
- Used experimental drugs for phosphodiesterase inquiry
- Personal
International Teaching
HEP
08.2015 - 06.2016
- Made international relations to create a universal teaching lesson
- Evaluated students' understanding of course material through examinations and in-depth essay writing
- Taught children 13-17 years of age science and chemistry
- Conducted research on teaching tactics to self-evaluate
- Modified lessons and curriculum to accommodate diverse learners by using strategies such as peer-assisted learning and group work
- Increased educational expertise and knowledge by participating in instructor-oriented workshops
Chemistry Supplemental Instructor
Instiller of Knowledge, Humboldt State
08.2014 - 05.2016
- Taught vital general chemistry information
- Developed learning goals and lesson plans
- Inspired and ignited a passion for chemistry
- Increased pass rate by 30%
- Recommended students for programs to enhance academic support through improved learning strategies
- Led discussions and activities centered around course content
- Projected high achievement levels to increase continued student enrollment
- Met with faculty members to discuss course content and progress
- Received and processed student feedback to assess, evaluate and improve program
Intern/fellow
National, Institute of Aging @ NIH
06.2015 - 08.2015
- Read and interpreted scientific articles which pertained to cardiovascular research
- Conducted cardiovascular research corresponding to autonomic responses
- Used live animal handling techniques and in vivo studies meant for research
- Purified compounds and performed drug response curves
- Identification and characterization of various compounds using various forms of technology
- Participated in workshops and presentations related to projects to gain knowledge
Reconnaissance Marine
United States Marine Corps
11.2007 - 11.2012
- Trained over 300 Marines for combat
- Supervised and led a team of 9 to Afghanistan
- Wrote orders and reports with sound and accurate judgement
- Adapted with flexibility to a demanding, changing work schedule with a minimum of 80 hrs/week
- Earned rank of Sergeant
- Purchased and received goods in excess of $300,000
- Developed protocols and procedures for traveling with Government Funds
- Upheld code of conduct and maintained professionalism in stressful situations
- Maintained awareness of subordinates' personal problems and shortcomings, supporting personal and professional growth
- Worked in dirty and uncomfortable conditions, sometimes in dangerous, deployed locations
- Provided strategic plotting and navigation for warfare areas
- Dealt with inter-company conflicts to remove obstacles to troop readiness
- Responded to and assisted in medical emergencies
Education
Master of Science - Pharmaceutical Sciences and Molecular Medicine
Washington State University
Spokane, WA 08.2020 - 08.2022
MBA -
Colorado State University
Fort Collins, CO 08.2019 - 12.2021
Bachelor of Science - Biochemistry And Molecular Biology
Humboldt State University
Arcata, CA 08.2013 - 05.2017
Skills
Western Blot,Immunoprecipitation, qPCR, PCR, ELISA, various Chromatography, scanning & electron microscopy
undefinedTimeline
Sr. Associate Process Development
Amgen Inc
07.2022 - 03.2024
Master of Science - Pharmaceutical Sciences and Molecular Medicine
Washington State University
08.2020 - 08.2022
Research Fellow
Washington State University
05.2020 - 05.2022
MBA -
Colorado State University
08.2019 - 12.2021
Research Specialist
San Diego State Research Foundation
05.2018 - 06.2020
Intern/fellow
National, Institute of Aging @ NIH
06.2017 - 08.2017
International Teaching
HEP
08.2015 - 06.2016
Intern/fellow
National, Institute of Aging @ NIH
06.2015 - 08.2015
Chemistry Supplemental Instructor
Instiller of Knowledge, Humboldt State
08.2014 - 05.2016
Bachelor of Science - Biochemistry And Molecular Biology
Humboldt State University
08.2013 - 05.2017
Reconnaissance Marine
United States Marine Corps
11.2007 - 11.2012
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