Todd Sickler
Phoenix,MD
Summary
Detail-oriented Laboratory Manager highly effective at keeping laboratories running smoothly and efficiently. Knowledgeable about managing supplies, equipment, and facilities. Pursuing new professional challenges with the opportunity to make a positive impact on laboratory operations and research success.
Overview
22
22
years of professional experience
Work History
BSL 3 Laboratory Manager
Department of Defense
01.2009 - Current
- Perform work as a biologist with graduate level training in biohazardous threat agents and emerging infectious disease
- CDC/DOJ Biological Select Agent Secret Security Clearance
- Maintains BSL-3 surety material
- Direct design, implementation and analysis of experimental protocols as BSL-3 laboratory manager such as laser measurements of aerosolized select agents genomic DNA extractions and microorganism identification via Real-Time PCR assays
- Develop and execute program management plans and critical path timelines on special projects such as various assay experiments
- Principle Investigator (PI) on JPEO funded projects
- Trained in Cell Culture
- Vaporous Hydrogen Peroxide (VHP) characterization and effects experiments on several bacterial spore species and material coupons
- Direct and oversee acquisition and procurement efforts
- Design Primer/Probe sequences for RT- QPCR assays
- Identification and characterization of pathogens and the incorporation on pathogenic microorganisms into various experimental programs and protocols
- The testing of instruments designed for the autonomous detection and identification of various microbial, viral and proteinaceous Select Agents within BSL-2 and BSL-3 laboratories
- Microorganism identification/quantification using ECL/LFI and Real-time PCR assays
- Provide expert consultation to staff and leadership on special projects related to biological threat agents
- Participate in intra- and inter-agency collaboration specific to biological threat agent research and development projects, including the Army’s Biological Surety Program
- Strategize on business development opportunities activities and work with leadership to effectuate joint ventures
- Contracting Officer Representative (COR)
- Government Macro Credit Card Holder
- Approving/Billing Official (A/BO).
- Interviewed, and trained lab technicians to perform experiments and analyze results
- Kept equipment maintained, facility clean, and organized and inventory stocked to maximize performance
- Kept laboratory in compliance with applicable guidelines and laws
- Designed processes to increase efficiency by minimizing error and reducing repetitive tasks
- Established team priorities, maintained schedules and monitored performance
- Streamlined and monitored quality programs to alleviate overdue compliance activities
- Developed detailed plans based on broad guidance and direction
- Successfully managed budgets and allocated resources to maximize productivity and profitability
- Maintained positive customer relations by addressing problems head-on and implementing successful corrective actions
- Set and oversaw quality assurance guidelines for laboratory work
Staff Scientist
Noblis
01.2006 - 01.2009
- Assist with design, implementation and analysis of experimental protocols as a Assistant BSL-3 laboratory manager such as Vaporous Hydrogen Peroxide (VHP) characterization and effects of bacillus spores and microorganism identification/quantification via ECL assays
- Oversee portion of DHS funded project, BAND and IBADS.
Life Scientist 1
SAIC
01.2004 - 01.2006
- Develop, design and execute protocols for subcloning into expression vectors, ligation and transformation reactions, protein purification, restriction mapping and gram-staining.
- Applied theories and methods of life sciences to interpret and perform analyses of experimental results
- Reviewed technical and professional publications and journals to stay current on recent literature and make more strategic research decisions
- Learned new laboratory techniques and applied expertise in carrying out enhanced experiments under supervision of senior lab members
- Worked as member of integrated project team in highly collaborative work environment
- Performed accurate quantitative analysis of targeted data research, collection and report preparation
Research Associate
Human Genome Sciences
01.2002 - 01.2004
- Established, designed and executed protocols for plasmid isolation, PCR cloning, ELISA assays and DNA sequencing.
- Gathered, arranged, and corrected research data to create representative graphs and charts highlighting results for presentations
- Validated incoming data to check information accuracy and integrity while independently locating and correcting concerns
- Helped team meet regulatory requirements by coordinating documentation and filings
- Set up equipment, organized inventory and maintained facilities
- Distilled large amounts of information and produced reports, spreadsheets, and documents outlining key details
Education
M.S. - Biohazardous Threat Agents and Emerging Infectious Disease
Georgetown University
Washington, D.C.05.2013
B.S. - Biology
University of Maryland, Baltimore County
Baltimore, MD05.2001
Associate Degree -
Community College of Baltimore County
Baltimore, MD05.1997
Skills
- Research Coordination
- Employee Supervision
- Experimental Design
- Sample Processing
- Data Interpretation
- Laboratory Management
- Procedure Monitoring
- Lab Equipment Maintenance
- Laboratory Work Competency
- Equipment Maintenance
- Standard Operating Procedures
- Cross-Functional Teamwork
- Assay Development
- Environmental Monitoring
- Hazardous Waste Management
Specialized Training
- Cell Culture Training
- Biological Safety Training
- Biomedical Waste Training
- Hazardous Waste Training
Poster Presentations Publications
- A Standard Method To Inactivate Bacillus anthracis Spores to Sterility via Gamma Irradiation, Cote CK, Buhr T, Bernhards CB, Bohmke MD, Calm AM, Esteban-Trexler JS, Hunter M, Katoski SE, Kennihan N, Klimko CP, Miller JA, Minter ZA, Pfarr JW, Prugh AM, Quirk AV, Rivers BA, Shea AA, Shoe JL, Sickler TM, Young AA, Fetterer DP, Welkos SL, Bozue JA, McPherson D, Fountain AW 3rd, Gibbons HS, Appl Environ Microbiol, 05/31/18, 10.1128/AEM.00106-18, 29654186, PMC5981071
- Selective detection of 1000 B. anthracis spores within 15 minutes using a peptide functionalized SERS assay, Farquharson S, Shende C, Smith W, Huang H, Inscore F, Sengupta A, Sperry J, Sickler T, Prugh A, Guicheteau J, Analyst, 2014, 139, 6366 – 6370
- Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory, Pan Y, Hill S, Santarpia J, Brinkley K, Sickler T, Coleman M, Williamson C, Gurton K, Felton M, Pinnick R, Baker N, Eshbaugh J, Hahn J, Smith E, Alvarez B, Prugh A, and Gardner W, Optics Express, 04/2014, 22, 7, 8165-8189
- Biosafety Level 3 Bio-aerosol Generation System, Lacirignola J, Richardson J, Martinez R, Froehlich E, Gennis A, Vanderbeek R, Wade M, Sickler T, Prugh A, Hung K
- Dial-A-Decon Solution Chemistry GAP Testing, Nickol R, Pfarr J, Shue M, Procell L, Sickler T
- Comparative Genomics of 2009 Seasonal Plague (Yersinia pestis) in New Mexico, Gibbons H, Krepps M, Ouellette G, Karavis M, Onischuk L, Leonard P, Broomall S, Sickler T, Betters J, McGregor P, Donarum G, Liem A, Fochler E, McNew L, Rosenzweig C, and Skowronski, PLoS ONE, 02/2012, 7, 2, e31604, 10.1371/journal.pone.0031604
- Validation of Photometric Measurement of Chlorine Dioxide Gas, Sickler T, Shah S, Smith L, Wallace L, Rastogi V, 11/2005
Areas Of Specialty
- Knowledge of Biological Threat Agents
- Program/Project Management
- Business Development
- Acquisition Support and Oversight
- Liaison with External Federal Agencies and Research & Development Organizations
- Center for Disease Control and Department of Justice
- Biological Select Agent Security (SSBI) Clearance
Timeline
BSL 3 Laboratory Manager
Department of Defense
01.2009 - Current
Staff Scientist
Noblis
01.2006 - 01.2009
Life Scientist 1
SAIC
01.2004 - 01.2006
Research Associate
Human Genome Sciences
01.2002 - 01.2004
M.S. - Biohazardous Threat Agents and Emerging Infectious Disease
Georgetown University
B.S. - Biology
University of Maryland, Baltimore County
Associate Degree -
Community College of Baltimore County
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