Tyler J. Hinthorn, MS
Joliet,IL
Summary
Experienced in molecular biology, immunology, and project management. Proficient in protein/RNA assays, immunohistochemistry, advanced staining techniques, and Keyence microscopy. Skilled in experimental design, molecular diagnostics, and therapeutic strategy development. Background in regulatory affairs and translational research, with a focus on cardiovascular disease, atherosclerosis, and immune system analysis.
Proven track record in leading research projects, from design to data analysis, with a strong passion for advancing scientific knowledge and improving patient outcomes.
Overview
7
7
years of professional experience
Work History
Graduate Research Intern
Human Immune Monitoring Shared Resource (HIMSR)
06.2024 - 08.2024
- Conducted research on Deployment-Related Respiratory Diseases (DRRD) in post-9/11 veterans under Dr. Kimberly Jordan's mentorship.
- Gained expertise in mass cytometry (CyTOF) for immune profiling of PBMCs, T cells, and monocytes to explore immune activation pathways.
- Analyzed data with FlowJo and CyTOF XT, identifying key markers of immune dysfunction and cytokine dynamics.
- Strengthened sample handling skills to ensure accuracy and consistency in experimental workflows.
- Identified immune activation markers (e.g., CD14+, CD11b+, HLA-DR+ monocytes) and linked immune dysfunction to environmental stressors like burn pits.
- Contributed to improved diagnostics and treatment strategies for veterans, enhancing data interpretation and problem-solving skills in disease progression research.
Laboratory Science Professional
University of Colorado Anschutz Medical Campus, Renal Division
01.2023 - 08.2024
- Contributed to vascular biology research in Dr. Mary Weiser-Evans Lab, studying PTEN’s role in smooth muscle cell differentiation and atherosclerosis.
- Investigated SMC reprogramming and adventitial progenitor cells (AdvSca1-SM) using murine models for vascular repair and ischemic revascularization.
- Assisted in lung cancer metastasis studies, identifying AdvSca1-SM cells' role in tumor progression and stromal progenitor cells in the tumor microenvironment.
- Conducted PCR, Western blotting, genotyping, breeding, and fate-mapping to analyze SMC signaling and reprogramming.
- Applied histology, tissue processing, and immunohistochemistry for cellular and tissue-level analysis.
- Collaborated on integrating vascular biology and cancer biology findings for therapeutic innovations.
- Strengthened data analysis and interpretation skills, contributing to impactful studies in vascular regenerative medicine and cancer research.
Laboratory Intern
City of Joliet Wastewater and Drinking Water Laboratory
05.2022 - 10.2022
- Gained expertise in EPA testing protocols, analyzing ammonia, BOD, fecal coliform, phosphorus, and TSS for regulatory compliance.
- Conducted lab testing and improved data management and quality assurance practices.
- Performed field sampling for accurate water quality monitoring.
- Strengthened technical skills in environmental testing and regulatory compliance.
Head Lifeguard
Joliet Park District
05.2017 - 03.2021
Education
Master of Science - Biomedical Sciences and Biotechnology
University of Colorado Anschutz Medical Campus
12.2024
Bachelor of Science - Biology
University of St. Francis
Joliet, Illinois12.2022
No Degree - Exercise Science And Physiology (Direct Admit PT)
Saint Louis University
St. Louis, MOSkills
- Regulatory Affairs Expertise
- CyTOF & Immune Profiling
- Cardiovascular & Atherosclerosis Research
- Molecular Biology & Genetics
- Scientific Communication & Writing
- Project Management & Organization
Accomplishments
Publications and Preprints:
- KLF4 in smooth muscle cell-derived progenitor cells is essential for angiotensin II-induced cardiac inflammation and fibrosis
Journal: Preprint, June 2024
- Smooth muscle-derived resident vascular adventitial progenitor cell contribution to cardiac fibrosis and inflammation is dependent on KLF4
Journal: Arteriosclerosis Thrombosis and Vascular Biology, May 2024
- PTEN protects against vascular smooth muscle cell pro-inflammatory dedifferentiation through metabolic re-wiring
Journal: Arteriosclerosis Thrombosis and Vascular Biology, May 2024
- Smooth muscle–derived adventitial progenitor cells direct atherosclerotic plaque composition complexity in a Klf4-dependent manner
Journal: JCI Insight, November 2023
- Confounding Effects of Tamoxifen: Cautionary and Practical Considerations for the Use of Tamoxifen-Inducible Mouse Models in Atherosclerosis Research
Journal: Arteriosclerosis Thrombosis and Vascular Biology, September 2023
- Smooth muscle-derived adventitial progenitor cells promote key cell type transitions controlling plaque stability in atherosclerosis in a Klf4-dependent manner
Journal: Preprint, July 2023
Timeline
Graduate Research Intern
Human Immune Monitoring Shared Resource (HIMSR)
06.2024 - 08.2024
Laboratory Science Professional
University of Colorado Anschutz Medical Campus, Renal Division
01.2023 - 08.2024
Laboratory Intern
City of Joliet Wastewater and Drinking Water Laboratory
05.2022 - 10.2022
Head Lifeguard
Joliet Park District
05.2017 - 03.2021
Master of Science - Biomedical Sciences and Biotechnology
University of Colorado Anschutz Medical Campus
Bachelor of Science - Biology
University of St. Francis
No Degree - Exercise Science And Physiology (Direct Admit PT)
Saint Louis University
Similar Profiles
Michael AnticoliMichael Anticoli
Director of Human Resources at Shared Resource CenterDirector of Human Resources at Shared Resource Center
Bobbie HowardBobbie Howard
Administrative Staff Analyst at Human Resources Administration/ Contract Monitoring Office (CMO)Administrative Staff Analyst at Human Resources Administration/ Contract Monitoring Office (CMO)
Lingeshwaran BLingeshwaran B
Desktop Support Engineer at Aleron Shared Resource India Pvt ltdDesktop Support Engineer at Aleron Shared Resource India Pvt ltd
Genaro ZamoraGenaro Zamora
Assistant General Manager at Noodles & CompanyAssistant General Manager at Noodles & Company
Brenda EscamillaBrenda Escamilla
Contract Billing Coordinator at Edward Don & CompanyContract Billing Coordinator at Edward Don & Company