Summary
Work History
Education
Skills
Relevant Publications
Timeline
Generic
Vincent Lu

Vincent Lu

Summary

Experienced and innovative Senior Research Scientist adept at high-throughput antibody discovery utilizing phage display, protein engineering and robotic lab automation. Spearheaded innovative research projects, enhancing efficiency and continuation of research grants. Proven ability to mentor and train students, while contributing to impactful scientific publications. Committed to advancing research through continuous improvement and technology integration.

Work History

Senior Research Scientist/Manager

University of Chicago
2010 - 2023
  • Collaborated with cross-functional teams from UCSF and the University of Toronto to establish and scale a high-throughput recombinant antibody platform (Recombinant Antibody Network)
  • Supported strategic partnerships with Celgene and Bristol Myers Squib, contributing to projects that secured multi-million dollar investments for antibody-based cancer therapies
  • Led phage display selections from hundreds of antigens to generate thousands of antibodies. Many of these high affinity binders were distributed to partners such as Absolute Antibody Ltd. for open use to facilitate research to other industries and academic entities
  • Managed a team of over 25 associates as well as maintaining efficient inventory oversight, procurement, and budget control to maintain seamless laboratory operations
  • Built a robust robotic automation platform that integrated a liquid handler, colony picker, incubator, plate hotel, PCR, plate readers , etc. This increased our output 100x and more importantly, it aided in reducing the error that can happen while doing these experiments manually

Molecular Biologist

Argonne National Laboratory
2007 - 2010
  • Project lead for a program to characterize core metabolic enzymes in a variety of prokaryotic and eukaryotic organisms. Developed methods and ligand libraries for implementation of a microwell plate-based fluorescence thermal shift assay for detection of ligand-protein binding.
  • Developed strategies for in vitro high-throughput cloning and production of proteins from Aspergillus niger (Zebrafish) in Pichia pastoris . Characterized a 96-well plate-based cDNA library of ~ 800 clones using a novel computational tool for coding sequence characterization.
  • Produced and characterized over 100’s bacterial and yeast expression clones for various classes of proteins from Shewanella oneidensis MR-1, including two-component system domains, conserved hypotheticals, transcription regulators, periplasmic cytochromes, and enzymes.
  • Analyze data and assist in the preparation of manuscripts.
  • Trained and supervised summer intern students in laboratory techniques and project experimental design.

Education

Certificate - Financial Management And Decision Making

University of Chicago
Chicago, IL
05-2015

Bachelor of Science - Biochemistry And Molecular Biology

Michigan State University
East Lansing, MI
05-2007

Skills

  • Phage Display
  • Protein Purification (AKTA and Benchtop)
  • PCR
  • Cloning
  • Sequencing (Sanger and NGS)
  • Robotic Lab Automation
  • Cell Culture
  • SPR
  • ELISA
  • Western Blots
  • Flow Cytometry
  • X-Ray Crystallography
  • DSF

Relevant Publications

Stuwe T, Bley CJ, Thierbach K, Petrovic S, Schilbach S, Mayo DJ, Perriches T, Rundlet EJ, Jeon YE, Collins LN, Huber FM, Lin DH, Paduch M, Koide A, Lu V, Fischer J, Hurt E, Koide S, Kossiakoff AA, Hoelz A. Architecture of the fungal nuclear pore inner ring complex. Science. 2015 Oct 2;350(6256):56-64. doi: 10.1126/science.aac9176. Epub 2015 Aug 27. PMID: 26316600; PMCID: PMC4826903.


Stuwe T, Correia AR, Lin DH, Paduch M, Lu VT, Kossiakoff AA, Hoelz A. Nuclear pores. Architecture of the nuclear pore complex coat. Science. 2015 Mar 6;347(6226):1148-52. doi: 10.1126/science.aaa4136. Epub 2015 Feb 12. PMID: 25745173; PMCID: PMC5180592.

Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S. Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2. Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):10010-10015. doi: 10.1073/pnas.1805343115. Epub 2018 Sep 17. PMID: 30224453; PMCID: PMC6176591.


Zhong N, Loppnau P, Seitova A, Ravichandran M, Fenner M, Jain H, Bhattacharya A, Hutchinson A, Paduch M, Lu V, Olszewski M, Kossiakoff AA, Dowdell E, Koide A, Koide S, Huang H, Nadeem V, Sidhu SS, Greenblatt JF, Marcon E, Arrowsmith CH, Edwards AM, Gräslund S. Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins. PLoS One. 2015 Oct 5;10(10):e0139695. doi: 10.1371/journal.pone.0139695. PMID: 26437229; PMCID: PMC4593582.

Larsen PE, Trivedi G, Sreedasyam A, Lu V, Podila GK, Collart FR. Using deep RNA sequencing for the structural annotation of the Laccaria bicolor mycorrhizal transcriptome. PLoS One. 2010 Jul 6;5(7):e9780. doi: 10.1371/journal.pone.0009780. PMID: 20625404; PMCID: PMC2897884.

Bauman A, Higdon R, Rapson S, Loiue B, Hogan J, Stacy R, Napuli A, Guo W, van Voorhis W, Roach J, Lu V, Landorf E, Stewart E, Kolker N, Collart F, Myler P, van Belle G, Kolker E. Design and initial characterization of the SC-200 proteomics standard mixture. OMICS. 2011 Jan-Feb;15(1-2):73-82. doi: 10.1089/omi.2010.0118. Epub 2011 Jan 21. PMID: 21250827; PMCID: PMC3110723.

Timeline

Senior Research Scientist/Manager

University of Chicago
2010 - 2023

Molecular Biologist

Argonne National Laboratory
2007 - 2010

Certificate - Financial Management And Decision Making

University of Chicago

Bachelor of Science - Biochemistry And Molecular Biology

Michigan State University

Similar Profiles

CREATE PROFILE