Vishal Bharathi
Cincinnati,OH
Summary
Dynamic research professional with extensive experience at Cincinnati Children’s Hospital Medical Center, specializing in iPSC differentiation and hematopoietic cell engraftment. Proven expertise in PCR and flow cytometry, coupled with strong organizational and communication skills, driving successful project outcomes in high-pressure environments. Committed to advancing scientific knowledge through innovative methodologies.
Overview
6
6
years of professional experience
Work History
Research Assistant III
Cincinnati Children’s Hospital Medical Center
Cincinnati, USA
02.2025 - Current
- Working with iPSC and patient-derived AML hiPSC lines.
- Differentiating iPSCs into bone marrow organoids and engraftable hematopoietic cells.
- Performing PCR, DNA extraction from blood, cytospin and staining.
- Maintaining detailed lab records and managing lab inventory.
- Trained in rodent handling, anesthesia & surgery, tagging, tailing, bleeding, and euthanasia.
- Experienced in organ harvesting, bone marrow cell extraction, and blood analysis in mice.
Senior Lab Assistant
Biotechnology Innovation center (BIC) at University of New Hampshire
Manchester
05.2023 - 12.2024
- Design and implement experiments to produce standard operating procedures.
- Maintain, installed, and calibrate scientific equipment for cell culture application.
- Oversaw Laboratory and technical operation, Inventory, Asset tracking, Lab safety and Biohazard materials.
- Managed operation and maintenance of bioreactors, ensuring optimal performance and efficiency.
- Differentiating Human Bone marrow derived MSC to Chondrocytes using in-house developed differentiation media.
- Carried out histological processing, including fixation, dehydration, embedding, sectioning, and staining, to assess chondrocyte using microscopy for analysis.
Research Assistant
University of New Hampshire
Manchester
08.2023 - 12.2023
- Cultured and expanded iPSCs and neural progenitor cells while ensuring optimal conditions for downstream applications.
- Orchestrated polymerization processes for gelatin-based biomaterials, optimizing formulations for specific applications.
- Performed extrusion-based bioprinting with gelatin-based biomaterials, producing high-quality constructs for research.
- Flow cytometry analysis.
Intern
LUCID LABORATORIES PVT LTD
Hyderabad, India
05.2019 - 06.2019
- Facilitated analysis, quality control, quality assurance, microbiological assessments, sample preparation, and autoclave sterilization, adhering to rigorous laboratory standards.
Education
Master of Science - Biotechnology
University of New Hampshire
Manchester, New Hampshire12.2024
Bachelor of Pharmacy -
Guru Nanak Institutions Technical Campus
Hyderabad, Telangana, INDIA05.2020
Skills
- DNA/RNA extraction
- Protein extraction and purification
- Western blotting
- SDS-PAGE gel electrophoresis
- PCR
- Plasmid purification
- ELISA
- Spectrophotometry
- Flow cytometry
- Chromatography
- HPLC
- FPLC (AKTA)
- Fluorescence microscopy
- Protein extraction
- Maintaining iPSC
- Differentiating
- Patient-derived AML hiPSC lines
- JMP
- MATLAB
- FIJI (Image J)
- Staining techniques
- Rodent handling
- Anesthesia & surgery
- Tag and Tail
- Bleeding
- Euthanasia
- Organ harvesting
- Bone marrow cell extraction
- Blood analysis in mice
- Cytospin
Projects
Protein Technology, Investigated impact of antibacterial agents and temperature on Blue Fluorescent Protein (BFP) production, resulting in a 30% increase in BFP yield. Cell Culture, Studied effects of camptothecin and cytochalasin D on cell migration and wound healing, leading to identification of key compounds with a 40% improvement in cell migration. Pluripotent Stem Cell, Developed ATRA-embedded PLA microspheres for iPSC-derived neuronal differentiation.
Extracurricular Activities
Travel, Passionate about exploring scenic, historically rich places, particularly enjoying train journeys to serene, uncrowded destinations. Outdoor Activities, Engaged in camping trips with friends, fostering teamwork and leadership in planning and executing trips. Anime, Follows anime to broaden cultural understanding and enhance critical thinking through various genres and storytelling styles.
Certificates And Achievements
- Certified in Biosafety Level 2 by the University of New Hampshire, ensuring adherence to strict laboratory safety standards.
- Presented research at the UNH Winter Symposium, with over 200 attendees, contributing to the advancement of stem cell research.
- Active participant in university events and poster competitions.
Timeline
Research Assistant III
Cincinnati Children’s Hospital Medical Center
02.2025 - Current
Research Assistant
University of New Hampshire
08.2023 - 12.2023
Senior Lab Assistant
Biotechnology Innovation center (BIC) at University of New Hampshire
05.2023 - 12.2024
Intern
LUCID LABORATORIES PVT LTD
05.2019 - 06.2019
Master of Science - Biotechnology
University of New Hampshire
Bachelor of Pharmacy -
Guru Nanak Institutions Technical Campus