Wanzhang Pan
West Lafayette,IN
Summary
Enthusiastic biochemist with 5 years research experience with a focus on applying chemical and
genetic tools to understand the underlying cell signaling biology for drug discovery.
• Conducted Interdisciplinary research incorporating organic chemistry, biochemistry, and cell
biology
• Competent independent researcher with a problem-solving mentality and strong oral
communication and leadership skills
Overview
7
7
years of professional experience
Work History
Ph.D. Graduate Research Assistant
Purdue University
West Lafayette, IN
08.2019 - 05.2024
- Developed strong problem-shooting and problem-solving skills through leading multiple research projects in cancer and neurodegenerative diseases drug discovery
- Developed leadership skills through training and guiding junior graduate students and undergrads
- Developed strong communication skills through interactions with academic collaborators and research colleagues
- Served as Lab Safety Officer (2021-2023).
Assistant Lab Technician
YZY BIOPHARMA CO LTD.
Wuhan, CHM
06.2017 - 09.2017
- Purified various proteins via affinity chromatography and cation exchange chromatography
- Sample characterization via Nanodrop, SDS-PAGE, GelDoc EZ Imager, Bicinchoninic acid assay (BCA assay).
Education
Ph.D. - Biochemistry
Purdue University
West Lafayette, IN05-2024
Bachelor of Science - Biochemistry And Molecular Biology
University of California, Santa Cruz
Santa Cruz, CA06-2018
Skills
Organic Chemistry
Synthesized novel patentable drug like molecules and ATP analog probes using modern organic
synthesis protocols
Purified Compounds using various column chromatography (DEAE Sephadex anion-exchange column,
silica gel column)
Characterized compounds via HPLC, NMR (H1, C13 and P31), and ESI Mass Spectrometry
Biochemistry
Molecular Cloning:
PCR, Transformation, DNA Extraction, Restriction Digestion, Ligation, Sanger Sequencing preparation and
analysis)
Expression and Purification of Recombinant Proteins:
Expressed recombinant proteins from bacterial expression systems (eg BL21-DE3, BL21-CodonPlus
(DE3)-RIPL) and Baculovirus expression system (Sf9 insect cells)
Purification of various recombinant proteins via Ni-NTA affinity and Glutathione Sepharose
chromatography, followed by FPLC (IEX or SEC)
Various In vitro Enzymatic Assays
phosphorylation assay, AMPylation/Adenylylation assay, peroxidase activity assay, kinase activity
assay
Co-immunoprecipitation (Co-IP) and Pull-down assays
Bradford Protein Assay
Others:
SDS-PAGE, Native PAGE
Southern Blot, Western Blot
Cell Biology
Maintained and passaged various mammalian cell lines (eg HEK293T, PHX-Ampho, C4-2, 22Rv1,
HT22, hybridoma E7)
Maintained and passaged Sf9 insect cells Transfected/infected Sf9 insect cells for recombinant protein expression using Bac-to-Bac Expression
System
Retroviral and lentiviral transfection of mammalian cells using calcium phosphate method
Computational Skills
Proficient at Snapgene, Pymol, Chemdraw, Mestrenova, Illustrator, GraphPad Prism
On-going Projects/Publications
• A Novel Link Between FICD and Oxidative Stress: Potential Implicationsin Neurodegenerative Diseases. Manuscript in preparation.
• Design and Synthesis of novel γ-Modified ATP Analog probes for in vitro phosphorylation and Proteomic Substrate Labeling. Manuscript in preparation.
• Yi Peng, Wanzhang Pan, Nan Wang, Jia-En Lu, Shaowei Chen, "Ruthenium Ion-Complexed Graphitic Carbon Nitride Nanosheets Supported on Reduced Graphene Oxide as High-Performance Catalysts for Electrochemical Hydrogen Evolution", ChemSusChem, 2018, 11, 130.
• Yi Peng, Eduardo Y. Hirata, Wanzhang Pan, Limei Chen, Jia En Lu, and Shaowei Chen, "Intraparticle Charge Delocalization through Conjugated Metal-Ligand Interfacial Bonds: Effects of Metal d Electrons", Chin. J. Chem. Phys., 2018, 31, 1.
Awards and Poster Presentations
• Undergraduate Dean's Honors List, University of California, Santa Cruz, 2014 – 2018
• Undergraduate Dean's Award, University of California, Santa Cruz, 2014 - 2018
• Undergraduate Research Award by Hierarchical Systems Research Foundation, 2017
• PULSe Orientation Faculty Poster Session, 2023
• Open House Recruiting Weekends Poster Presentation, 2023
• PULSe Orientation Faculty Poster Session, 2022
• PULSe Orientation Faculty Poster Session, 2021
References
- Scott McLuckey, John A. Leighty Distinguished Professor of Chemistry, Department of Chemistry, Purdue University, West Lafayette, IN, (765) 494-5270, mcluckey@purdue.edu
- Humphrey Lotana, Phd Candidate in Chemistry (Biochemistry Division), Department of Chemistry, Purdue University, West Lafayette, IN, (207) 409-6446, hlotana@purdue.edu
- Chittaranjan Das, Professor of Biochemistry, Department of Chemistry, Purdue University, West Lafayette, IN, (765) 494-5478, cdas@purdue.edu
- Shiliang Tian, Assistant Professor of Chemistry, Department of Chemistry, Purdue University, West Lafayette, IN, (765) 494-5467, sltian@purdue.edu
Timeline
Ph.D. Graduate Research Assistant
Purdue University
08.2019 - 05.2024
Assistant Lab Technician
YZY BIOPHARMA CO LTD.
06.2017 - 09.2017
Ph.D. - Biochemistry
Purdue University
Bachelor of Science - Biochemistry And Molecular Biology
University of California, Santa Cruz
Similar Profiles
Bahiyyih BakerBahiyyih Baker
Senior Test Administrator at Purdue Testing Services, Purdue UniversitySenior Test Administrator at Purdue Testing Services, Purdue University
Denis Portal-HernandezDenis Portal-Hernandez
Team Leader at Purdue UniversityTeam Leader at Purdue University
Jade GermannJade Germann
ZYRO Student (Summer) at Purdue UniversityZYRO Student (Summer) at Purdue University
Cindy BlackCindy Black
Senior Administrative Assistant at Purdue UniversitySenior Administrative Assistant at Purdue University
Dominic GalanteDominic Galante
Landscaper at Self EmployedLandscaper at Self Employed