Xin Chen
Madison
Overview
13
13
years of professional experience
Work History
Principal Scientist
Invenra
11.2021 - Current
- Set up and maintain an MS-based laboratory
- Develop MS methods for structural characterization of antibodies, including intact mass analysis, protein sequencing, PTM analysis, and native MS methods
- Lead bispecific antibody forced degradation studies, pre-formulation studies, and developability studies
- Lead B-body bispecific antibody platform characterization
Senior R&D Scientist
Scientific Protein Laboratories
02.2021 - 09.2021
- Optimizing process to purify defibrotide from swine intestine mucosa
- Establish lab scale process for crude DNA isolation from Peptone
- Develop and perform numerous analytical assays (SEC-UV, SECMALS, SAX HPLC, relative potency, DNA content) on the acid digested DNA to determine processing equivalency to reference standard of defibrotide
Senior Scientist
Catalent Pharma Solutions
02.2016 - 12.2020
- Create the LCMS laboratory and lead mass spectrometry group
- Develop LCMS platform assays to characterize critical attributes of monoclonal antibodies, including sequence confirmation, sequence variant identification, qualitative and quantitative analysis of post-translational modifications
- Develop LCMS based analytical characterization strategies to support drug development activities, including clone selection, upstream condition optimization, downstream purification optimization, forced degradation and comparability studies
- Oversee analytical techniques for biologics release/characterization tests, including LC/MS, HPLC (SEC, RP, HIC, and IEX), and CE
- Implement CD, DSC, DLS, CE instruments and methods to expand department capability
- Lead mAb formulation, stability, forced degradation, and comparability studies
Senior Scientist
Pharmaceutical Product Development (PPD)
05.2015 - 02.2016
- Develop and validate LC-UV-MS methods to separate and quantify proteins and peptides
- Write project proposals/plans
Researcher
University of Wisconsin-Madison
03.2013 - 04.2015
- Develop and validate LC/MS methodologies to extract, separate, and quantify polyphenols in plants
- Develop MALDI-TOF MS to identify anthocyanidin chemical structures
- Develop Super Fluidic Chromatography (SFC) to separate anthocyanidin polymers
Education
Ph.D. - Analytical Chemistry
Brigham Young University
Provo, UT, USA01.2011
Post-doctoral Research - undefined
University of Wisconsin-Madison
Madison, WI, USA02.2013
Skills
- Extensive expertise in leading LCMS method development to characterize antibodies
- Proficient in LC method development, qualification, and validation for antibodies
- Extensive hands-on experience of LCMS, SEC, IEC, RP, HIC, CE-SDS, cIEF
- Lead a team to characterize antibodies, perform forced degradation and formulation studies
- Ability to work in a high-paced team environment and in cross functional teams
- Enthusiasm in science and technology, excellent written and verbal communication skills
Timeline
Principal Scientist
Invenra
11.2021 - Current
Senior R&D Scientist
Scientific Protein Laboratories
02.2021 - 09.2021
Senior Scientist
Catalent Pharma Solutions
02.2016 - 12.2020
Senior Scientist
Pharmaceutical Product Development (PPD)
05.2015 - 02.2016
Researcher
University of Wisconsin-Madison
03.2013 - 04.2015
Post-doctoral Research - undefined
University of Wisconsin-Madison
Ph.D. - Analytical Chemistry
Brigham Young University
Publications
- Chen, X. et al. Investigation of Dimers in Bispecific antibody Generated by B-Body Platform Using Native Mass Spectrometry, Manuscript in preparation, Journal of the American Society for Mass Spectrometry
- Chen, X. et al. Acidic and Basic Charge Variants Characterization of Bispecific antibody Generated by B-Body Platform, Manuscript in preparation, Journal of Pharmaceutical Science
- Chen, X. et al. Forced Degradation Study Revealed Distinct Stability of Bispecific Antibody Generated by B-Body Platform, Manuscript in review, Journal of Pharmaceutical and Biomedical Analysis
- Chen, X.; Parker, J.; Reed, J. et al. Validation of HPLC Assay for the Identification and Quantification of Anthocyanins in Black Currants. Analytical Methods, 2014, 6, 8141-8147.
- Peng, Y.; Chen, X.; Ge, Y. et al. In-Depth Proteomic Analysis of Human Troponyosin by Top-down Mass Spectrometry, Journal of Muscle Research and Cell Motility, 2013, 34, 199-210.
- Chen, X.; Ge, Y. High-Resolution SEC for Top-Down Proteomics, Proteomics, 2013, 13, 2563-2566.
- Peng, Y.; Chen, X.; Ge, Y. et al. Top-Down Targeted Proteomics for Deep Sequencing of Tropomyosin Isoforms, Journal of Proteomics Research, 2013, 12, 187-198.
- Chen, X.; Peng, Y.; Ge, Y. et al. Rapid Purification and High-Resolution MS Characterization of Human Salivary Amylase, Analytical Chemistry, 2012, 84, 3339-3346.
- Chen, X.; Tolley, H. D.; Lee, M. L. Monolithic Capillary Columns Synthesized from a Single Phosphate-Containing Dimethacrylate Monomer for Cation-Exchange Chromatography of Peptides and Proteins. Journal of Chromatography A, 2011, 1218, 4322-4331.
- Chen, X.; Tolley, H. D.; Lee, M. L. Weak Cation-Exchange Monolithic Column for Capillary Liquid Chromatography of Peptides and Proteins. Journal of Separation Science, 2011, 34, 2063-2071.
- Chen, X.; Tolley, H. D.; Lee, M. L. Preparation of Zwitterionic Polymeric Monolithic Columns for Hydrophilic Interaction Capillary Liquid Chromatography. Journal of Separation Science, 2011, 34, 2088-2096.
- Chen, X.; Tolley, H. D.; Lee, M. L. Polymeric Cation-Exchange Monolithic Columns Containing Phosphoric Acid Functional Groups for Capillary Liquid Chromatography of Peptides and Proteins. Journal of Chromatography A, 2010, 1217, 3844-3854.
- Chen, X.; Tolley, H. D.; Lee, M. L. Polymeric Strong Cation-Exchange Monolithic Column for Capillary Liquid Chromatography of Peptides and Proteins. Journal of Separation Science, 2009, 32, 2565-2573.