Yan Xu
Chicago,USA
Summary
Extensive research experience in the fields of molecular biology, cell biology, biochemistry, and genetics, coupled with solid and well-trained technical skills. Committed to innovative Translational Research with a focus on advancing disease treatment. Demonstrated dedication to pioneering groundbreaking diagnostic tools and therapeutics for personalized medicine.
Overview
27
27
years of professional experience
Work History
Post-Doctoral Research Fellow
Department of Internal Medicine, Rush University Medical Center
2021.01 - Current
- Developed a novel small therapeutic peptide, poised with remarkable potential to target αvβ3 integrin-mediated kidney injury and combat albuminuria in the context of chronic kidney disease. Administration of this peptide significantly reverses the albuminuria in proteinuric mice models.
Research Associate III
Department of Pediatrics, Ann & Robert H Lurie Children’s Hospital
2018.01 - 2021.01
- Identified sgRNAs with exceptional knockdown effectiveness (above 85%) targeting genes associated with pulmonary hypertension in rat lung endothelial cells.
- Developed in vivo gene knock-down using CRISPR techniques for treating pulmonary hypertension using young rats as a model system.
Staff Scientist
Department Of Genetics, Washington University In Saint Louis
2017.01 - 2018.01
- Delineated the role of METT-10, a U6 small nuclear RNA methyltransferase homolog in C. elegans, in promoting stem cell fate in C. elegans germline.
Postdoctoral Research Associate
Department of Biological Sciences, University of Wisconsin-Milwaukee
2010.01 - 2017.01
- Identified genes function downstream of Netrin and SLT-1 axon guidance signaling during development using C. elegans as a genetic model system.
Research Technician
Department of Biological Science and Biotechnology, Tsinghua University
2000.01 - 2001.01
- Analyzed the activity and structure of horseradish peroxidase in an organic solvent.
- Handled all troubleshooting tasks on malfunctioning lab equipment working with manufacturers to resolve problems.
Technician
Beijing Sai-Yin-Si Institute of Biotechnology
1997.01 - 2000.01
- Involved in the development of an ELISA kit for the pathological diagnosis of acute myocardial infarction (cTnI), targeting troponin I for precise detection and diagnosis.
Education
Ph.D. - Neurodevelopment
Rutgers University-Robert Wood Johnson Medical School
Piscataway, NJ 0885412.2009
Bachelor of Science - Biochemistry
Nanjing University
Nanjing, Jiangsu, China, 21009312.1997
Skills
- Cell Biology: CRISPR editing, Mammalian cell culture/tissue culture (maintaining and storing cell lines-immortalized and primary cell culture), Isolation of mouse lung primary endothelial cells, Isolation and culture of mouse lung primary fibroblast cells, isolation of mouse primary podocyte cells, transient or stable transfections (lentiviral vector), small interference RNA/shRNA, and cell-based reporter gene assays (luciferase reporter), cell proliferation assay, co-culture, confocal microscopy, fluorescence microscopy, cell proliferation assay, ELISA, and immunofluorescence.
- Molecular Biology: PCR, real-time or RT-qPCR, RNA, molecular cloning, ligation independent/Gibson cloning, site-directed mutagenesis, RNA/genomic/plasmid DNA (nucleic acid) extraction.
- Biochemistry: Recombinant protein expression (bacteria), Protein purification, GST pull-down assay, Co-immunoprecipitation, SDS-PAGE/native-PAGE, Western blot.
- Genetics: In vivo mutagenesis and genetic screening, Epistasis analysis (in c. elegans model)
- Software: Microsoft Office (Word, Excel, PowerPoint), GraphPad Prism (biostatistics), SnapGene, ImageJ
- Time Management
Accomplishments
2010, UWM Research Foundation Fellowship
Presentations
- Oral presentation, Kidney Week 2023 Annual Meeting, 11/03/23, A novel small therapeutic peptide with potential for treating αvβ3-mediated glomerular damage.
- Poster presentation, Kidney Week 2022 Annual Meeting, 11/04/22, Therapeutic potential of ICOSL for treating αvβ3-mediated glomerular damage
- Invited Symposium talk, Department of Biological Sciences colloquium at UWM 2015, SYD-1 promotes multiple developmental steps leading to neuronal connectivity.
Publications
- Xu Y, Cao Y, Koh KH, Kliewe F, Spear R, Mangos S, Endlich N, Reiser J, Hahm E. A novel small therapeutic peptide with the potential for treating αvβ3-mediated albuminuria. (in submission)
- Xu Y, Quinn C (2016). Transition between synaptic branch formation and synaptogenesis is regulated by the lin-4 microRNA. Dev. Biol. 2016 Dec 1;420(1):60-66
- Xu Y and Quinn CC (2016). SYD-1 promotes multiple developmental steps leading to neuronal connectivity. Mol Neurobiol. 2016 Dec;53(10):6768-6773.
- Xu Y, Taru H, Jin Y and Quinn CC (2015). SYD-1C, UNC-40 (DCC) and SAX-3 (Robo) function interdependently to promote axon guidance by regulating the MIG-2 GTPase. PLoS Genet. 11(4): e1005185
- Xu Y and Quinn CC (2012). MIG-10 Functions with ABI-1 to Mediate the UNC-6 and SLT-1 Axon Guidance Signaling Pathways. PLoS Genet. 8(11): e1003054
- Xu Y, Ren XC, Quinn CC, and Wadsworth WG (2011). Axon response to guidance cues is stimulated by acetylcholine in Caenorhabditis elegans. Genetics. 189(3): 899-906
- Zhou HW, Xu Y, and Zhou HM (2002). Activity and conformational changes of horseradish peroxidase in trifluoroethanol. Biochem. Cell. Bio. 80: 205-213
Timeline
Post-Doctoral Research Fellow
Department of Internal Medicine, Rush University Medical Center
2021.01 - Current
Research Associate III
Department of Pediatrics, Ann & Robert H Lurie Children’s Hospital
2018.01 - 2021.01
Staff Scientist
Department Of Genetics, Washington University In Saint Louis
2017.01 - 2018.01
Postdoctoral Research Associate
Department of Biological Sciences, University of Wisconsin-Milwaukee
2010.01 - 2017.01
Research Technician
Department of Biological Science and Biotechnology, Tsinghua University
2000.01 - 2001.01
Technician
Beijing Sai-Yin-Si Institute of Biotechnology
1997.01 - 2000.01
Ph.D. - Neurodevelopment
Rutgers University-Robert Wood Johnson Medical School
Bachelor of Science - Biochemistry
Nanjing University
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