Yang Chen
Ann Arbor,MI
Summary
Enthusiastic lab research eager to contribute to team success through hard work, attention to detail, and trained laboratory skills. Motivated to learn, grow, and excel in life science.
Overview
6
6
years of professional experience
Work History
Student Researcher
Isom Lab
09.2023 - Current
- Planned, modified and executed research techniques, procedures and tests.
- Designed primer and carried out site-directed mutagenesis of R60C mutation in human SCN1B cDNA plasmid
- Extract plasmid using miniprep and midiprep.
- Cultured HEK293 cells and measured endogenous RNA and protein levels of sodium channel β1 subunits using RT-PCR and western blot.
- Carried out transfection in HEK293 cells with different SCN1B plasmids.
- Carried out whole-cell patch clamp on transfected cells and collected data.
- Analyzed data using clampfit and prism
- Made presentations about own project and presented journal clubs
- Learned to perform brain slice.
Research Intern
W. McIntyre Burnham's Lab
05.2022 - 06.2023
- Prepared drugs and different test-related solutions.
- Handled mice and conducted part of drug injections in mice.
- Assisted with Maximal electroshock seizure (MES) testing in mice model.
- Conducted Kindling test in mice and monitored EEG of mice to determine seizure threshold.
- Gathered, arranged and corrected research data to create representative graphs (e.g. dose-response curves) and charts highlighting results for presentations.
- Attended meetings (laboratory regular meetings and Fellows' Meetings of EpLink) and presentations to learn more details about antiseizure drugs and treatments.
- Gave presentations in Journal Club to share and discuss up-to-date articles.
- Planned, modified and executed research techniques, procedures and tests.
Intern
Zybio Inc.
05.2021 - 08.2021
- Gathered, arranged and corrected research data to create representative graphs and charts highlighting results for presentations.
- Attended meetings and presentations to learn more details about Immunohistochemistry and Liquid-based cytology.
- Developed new formula of Hematoxylin solution used as stain in Immunohistochemistry tests, while monitoring its strength over time.
- Carried Immunohistochemistry tests for tumor tissues using more than a hundred types of antibodies both by hand and machines to test efficacy of them for different cancer types.
- Took pictures for all test results using microscope and related software.
- Assisted with maintenance of machines in laboratory.
Research Volunteer
Microbial Metabolism Laboratory at Shanghai Jiao Tong University
07.2018 - 08.2018
- Helped with preparation of culture medium for incubation of Escherichia coli and fermentation.
- Helped with monitoring and ensuring proper function of fermentation machines.
- Assisted with data recording include concentration of glucose and other chemicals using HPLC.
Education
Master of Science - Pharmacology
University of Michigan - Ann Arbor
Ann Arbor, MI08.2024
Bachelor of Science - Specialist: Pharmacology & Major: Biochemistry
University of Toronto
Toronto, ON06.2023
Skills
- Experience of working in biochemical labs both in university and company
- Familiar with electrophysiology techniques including whole-cell patch clamping
- Familiar with many in vitro laboratory techniques including western blot, DNA & RNA extraction, site-directed mutagenesis, cell culturing, transfection, and immunohistochemistry
- Familiar with in vivo laboratory techniques from mice handling and injections to mice seizure models ( Maximal electroshock seizure model & Kindling model)
- Data Collection and Analysis (Prism)
- IT Skills: MS Office (Excel, Word, PowerPoint), programming languages (basic Python, Java, R)
Timeline
Student Researcher
Isom Lab
09.2023 - Current
Research Intern
W. McIntyre Burnham's Lab
05.2022 - 06.2023
Intern
Zybio Inc.
05.2021 - 08.2021
Research Volunteer
Microbial Metabolism Laboratory at Shanghai Jiao Tong University
07.2018 - 08.2018
Master of Science - Pharmacology
University of Michigan - Ann Arbor
Bachelor of Science - Specialist: Pharmacology & Major: Biochemistry
University of Toronto
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