Summary
Overview
Work History
Education
Skills
Qualifications Summary
Publications
References
Timeline
Generic

Yetty Irwan

Fort Collins,CO

Summary

Highly skilled scientist with over 15 years of experience in biotechnological research and development. Proven expertise in designing and conducting experiments, with a strong
background in cell and tissue culture, DNA manipulation, animal studies, and data analysis. Adept at leading teams through complex projects, demonstrating exceptional problem-solving abilities and collaboration. Eager to contribute to cutting-edge research and innovation.

Overview

23
23
years of professional experience

Work History

Professional Cornhole Player

American Cornhole League
09.2019 - 08.2024
  • Participated in national cornhole tournaments, enhancing the sport's popularity.
  • Led cornhole clinics and represented sponsors, promoting the American Cornhole League.

Senior Development Scientist

Beckman Coulter Inc.
Fort Collins/Loveland , CO
01.2017 - 04.2019
  • Technical lead of a small team of scientists developing and testing reagents for a new cell viability analyzer instrument.
  • Involved in all the development phases (feasibility, characterization, and verification) of the reagents, including writing test plan and protocols, problem solving, data analysis and presentation, and writing reports.
  • Represented the Reagents team in collaboration with all the other leads in the project, i.e. Hardware, Software, Supply Chain, QA, Marketing, etc., to meet project’s deadlines.

Senior Development Scientist

Beckman Coulter Inc.
Miami , FL
11.2014 - 12.2016
  • Tested the feasibility of reagents applications on a newly developed flow cytometer followed by characterization, verification, and validation of the system (including writing protocols, troubleshooting, data analysis, data presentation/review, and writing reports).
  • Led a core team in 2015 that successfully launched a sheath fluid product for a flow cytometer and extended the expiration dating of the sheath fluid based on the real-time stability data.

Senior Development Scientist

Beckman Coulter Inc.
Miami , FL
12.2010 - 10.2014
  • Designed, formulated, and tested multicolor panels of fluorescence-conjugated antibodies for flow cytometry applications (including data analysis using Kaluza and/or FlowJo software, vialing, packaging and shipping antibodies cocktails).

Research Associate

Nevada Cancer Institute
Las Vegas , NV
12.2008 - 09.2009
  • Analyzed the pathophysiology of Interleukin-2-induced neurologic toxicity using behavioral study, immunohistochemistry, dynamic contrast-enhanced MRI, and knockout mice.

Graduate Assistant

University of Utah
Salt Lake City , UT
08.2003 - 12.2008
  • Developed quantitative assays to analyze Interleukin-2-induced vascular toxicity and vascular leak in the mouse brain.

Junior Laboratory Technician

Penn State College of Medicine
Hershey , PA
07.2002 - 06.2003
  • Overseeing the knock-out and congenic mice colony (i.e. breeding, genotyping, collecting tissue samples needed for studies).

Summer Research Intern

Elizabethtown College
Elizabehtown , PA
06.2001 - 08.2001
  • Cloned new cell lines to express an inducible Simian Virus 40 Large T Antigen.

Education

Doctor of Philosophy - Experimental Pathology

University of Utah
UT
09-2009

Bachelor of Science - Biotechnology

Elizabethtown College
PA
05-2002

Skills

  • Cell and tissue culture
  • Multicolor flow cytometry panel design and data analysis
  • Computer: Microsoft Office, ImageJ, Prism, JMP, FlowJo, and Kaluza
  • Animal handling (expertise in mice)
  • Cloning cells and use of reporter genes (luciferase assay and β-galactosidase)
  • DNA purification
  • PCR and RT-PCR
  • Southern blot
  • Cytotoxic lymphocyte assay
  • Proliferation assay (thymidine incorporation and BrdU staining)
  • Immunoprecipitation
  • Immunoblotting
  • Immunohistochemistry
  • Data collection and analysis of dynamic contrast-enhanced MRI

Qualifications Summary

Over fifteen years of research experience – cloning cells, developed new experimental methods to study the effect and mechanisms of immunotherapy agents in the mouse brain, multicolor flow cytometry, and cell viability analyzer reagent development. Extensive experience of molecular and cellular biology techniques. Two years of teaching experience – an avid tutor during undergraduate study and a teaching assistantship during graduate study, plus some coaching experience in cornhole. Strong background knowledge in Molecular and Cell Biology, Immunology, and Neuroscience, and excellent computer skills.

Publications

  • Demonstration of Infrared Laser Applications Using Beckman Coulter CytoFLEX S Flow Cytometer; CYTO Meeting, Seattle, June 2016.
  • Use of an IR Laser to Excite and Avalanche Photodiode to Detect IR and Near-IR Dyes on the CytoFLEX Flow Cytometer; CYTO Meeting, Seattle, June 2016.
  • Demonstration of a 7-color TBNK Application with Viability Dyes Using Beckman Coulter CytoFLEX Flow Cytometer; CYTO Meeting, Glasgow, Scotland, June 2015.
  • Designing Multi-color Panels for T/Treg Populations using FITMaN Guidelines: A Case Study; FOCIS Meeting, Washington D.C., June 2011.
  • Quantitative Analysis of Cytokine-induced Vascular Toxicity and Vascular Leak in the Mouse Brain; J. Immunol Methods 349: 45-55, 2009.
  • Evaluation of Interleukin-2 Neurovascular Toxicity Using DCE-MRI; 17th Scientific Meeting of the International Society for Magnetic Resonance in Medicine, Hawaii, April 2009.
  • Construction of a Cell Line Expressing an Inducible Simian Virus 40 Large T Antigen for the Study of Ribosomal Gene Activation; 78th Annual Meeting of the Pennsylvania Academy of Science, Pocono, PA, April 2002.

References

References available upon request.

Timeline

Professional Cornhole Player

American Cornhole League
09.2019 - 08.2024

Senior Development Scientist

Beckman Coulter Inc.
01.2017 - 04.2019

Senior Development Scientist

Beckman Coulter Inc.
11.2014 - 12.2016

Senior Development Scientist

Beckman Coulter Inc.
12.2010 - 10.2014

Research Associate

Nevada Cancer Institute
12.2008 - 09.2009

Graduate Assistant

University of Utah
08.2003 - 12.2008

Junior Laboratory Technician

Penn State College of Medicine
07.2002 - 06.2003

Summer Research Intern

Elizabethtown College
06.2001 - 08.2001

Doctor of Philosophy - Experimental Pathology

University of Utah

Bachelor of Science - Biotechnology

Elizabethtown College
Yetty Irwan