YONGTING CHEN
Malden,MA
Summary
I am seeking a position in a neuroscience lab to transition into neurobiology research before pursuing a Ph.D. I have acquired comprehensive experience in immunofluorescence, encompassing both probe preparation and image analysis. Moreover, I am adept in an array of molecular biology techniques, including PCR, Western Blot, and DNA sequencing. My professional journey has instilled in me a robust problem-solving acumen, an ability to decipher intricate data sets, and a commitment to meticulous data documentation.
Overview
5
5
years of professional experience
Work History
Analytical Development Co-Op
Synlogic
07.2023 - 12.2023
- I worked on a development project on lysis assay with β-galactosidase and performed assay on different samples and programs
- I have gained experience in operation of lab instruments including the cellometer, centrifuge, plate reader, and microfluidizer.
- Enhanced project efficiency by streamlining communication channels and implementing time-saving tools.
- Provided comprehensive support to team members, fostering a collaborative and inclusive work environment conducive to success.
Research Assistant
Center for Systems Neuroscience
01.2023 - 06.2023
- I conducted experiments related to functional genomics and the pathophysiology of Alzheimer's disease, specifically the molecular mechanisms of the tau protein
- I employ a wide range of laboratory skills including fluorescence microscopy, cell and tissue culture experiments, fixation, and immunohistochemistry, gel electrophoresis, blotting techniques, PCR,and staining techniques
- Participated actively in regular meetings with fellow researchers to discuss project updates, challenges faced, and lessons learned during ongoing activities.
Research Associate
Center For Biomedical Mass Spectrometry
12.2020 - 06.2023
- In my independent project, I utilize electron activated dissociation (ExD) methods, such as electronic excitation dissociation (EED) to generate structurally informative tandem mass spectra for both off-line and on-line with various glycan separation methods
- I have successfully achieved 3-AP coupling and methylation on glycan standard
- I have evaluated different purification strategies, including SCX, SEC and PGC, for the experiment
- I have prepared Me-3-AP labeled glycans and FRAGS-II-labeled glycans independently
- I have also been trained to operate MALDI-TOF MS and ESI-FTICR MS for analysis
- I have used commercial and custom software, including GlycoWorkbench and Cheraw, for spectral interpretation
- I integrated analytical and bioinformatics approaches for characterization of complex glycan mixture.
Research Assistant I
Biology Department
09.2021 - 05.2021
- I have played a pivotal role in investigating the localization of SARS-CoV-2 proteins within DF-1 cells by conducting indirect immunofluorescence assays to precisely visualize the localization of FLAG-tagged SARS-CoV-2 proteins in DF-1 cells.
- A significant aspect of my work has involved orchestrating transfection experiments, closely observing shifts in cell morphology, particularly in DF-1 cells transfected with the ORF9b protein
- Beyond these areas, I have extensive experience in immunofluorescence, including probe preparation and image analysis
- Moreover, I am proficient in various molecular biology techniques such as PCR, Western Blot, and DNA sequencing.
- I had the privilege of contributing to cutting-edge projects involving CRISPR-associated immune systems in bacteria, specifically focusing on CRISPR-Cas9 technology for precise genome editing and mutation studies in Saccharomyces cerevisiae
- My responsibilities included designing and testing guide RNA sequences, conducting mutation frequency analysis, and exploring repair mechanisms associated with CRISPR-Cas9-induced double-strand breaks.
Research Trainee
Boston Children Hospital
05.2019 - 12.2019
- I participated in the differentiation process of TSC2-/- and TSC2+/- iPSCs into kidney cells and organoids, aiming to mimic characteristics relevant to TSC-associated kidney tumors, particularly angiomyolipomas (AML)
- I also contributed to optimizing differentiation protocols and experimental procedures, collaborating with team members to address challenges and improve efficiency
- Developed proficiency in iPSC culture techniques, cell differentiation protocols, and data analysis methods, enhancing skills in molecular biology and stem cell research.
- Enhanced research efficiency by streamlining data collection processes and implementing advanced analytical tools.
Education
Master of Science in Biology - Biology, Cell, Molecular and Genetic
Boston University
Boston, MA05.2023
Bachelor of Arts - Biology, Cell, Molecular and Genetic
Boston University
Boston, MA05.2023
Skills
- Cell Culture
- SDS-PAGE
- Biochemistry
- Research
- Data Analysis
- Pipetting
- Bacterial Transformation
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Polymerase Chain Reaction (PCR)
- Agarose Gel Electrophoresis
- Protein Purification
- Western Blotting
- Immunohistochemistry
- Report Writing
- Experimental Design
- Troubleshooting
- Public Speaking
- Strategic Planning
- Fluency in all Microsoft Office applications (Excel, Word, PowerPoint, etc)
Accomplishments
Boston University National Honor Society Alpha Alpha Alpha 2023
Boston University Newbury Center Summer Research Award 2022
Boston University Undergraduate Research Award (June 2021- 2022)
Boston University Dean’s List (2019-2023)
Presentations
- 69th American Society for Mass Spectrometry (ASMS) Conference, Philadelphia, PA, 11/2021
- Boston University Undergraduate Research Opportunities Program (UROP) Symposium, Boston, MA, 10/2021-2022
- Boston University National First-gen College Celebration, Boston, MA, 11/2022
- Yale Undergraduate Research Association Intercollegiate Symposium, New Haven, CT, 04/2023
Relevant courses
- Genetics Lab
- Neuro Models Memory
- Neuro Motivation Learning
- Human Brain Mapping
- Neural System 1 & 2
- Advanced Evolutionary Analysis
- Animal Behavior
- Sensory Neurobiology
- Master Research
- Drug and Behavior
- Neural Circuits
- Nerves Tumors
- Neurobiology Disease
- Memory System
- Neuroethology
- Molecular Biology Lab
- Evolution
- Molecular Biology
- Biochemistry 1
- Cell Biology
- General Chemistry 1 & 2
- Physics (Mechanics, Electricity & Magnetism)
Timeline
Analytical Development Co-Op
Synlogic
07.2023 - 12.2023
Research Assistant
Center for Systems Neuroscience
01.2023 - 06.2023
Research Assistant I
Biology Department
09.2021 - 05.2021
Research Associate
Center For Biomedical Mass Spectrometry
12.2020 - 06.2023
Research Trainee
Boston Children Hospital
05.2019 - 12.2019
Master of Science in Biology - Biology, Cell, Molecular and Genetic
Boston University
Bachelor of Arts - Biology, Cell, Molecular and Genetic
Boston University
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