Summary
Overview
Work History
Education
Skills
Languages
Timeline
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YUANJIE LIN

Baltimore,Maryland

Summary

  • current Johns Hopkins biotechnology student learning the biochemistry dept
  • skilled with protein and molecular lab assay and full of passion to be trained with more laboratory technique
  • Highly-motivated to take on new challenges with strong problem solving skills. Adept at working effectively unsupervised and quickly mastering new skills.

Overview

1
1
year of professional experience

Work History

Industrial R&D Intern

CanSino Biologics Inc
Shanghai, China
05.2023 - 08.2023
  • Assisted with developing and testing the properties of lipid nanoparticles
  • Conducted Plasmid Maxi Preparation, mRNA amplification, LNP lipid solution preparation, and mRNA-LNP sample preparation
  • Performed mRNA-LNP sample atomization, encapsulation rate detection, DLS, Zeta potential detection, and Pka detection.

Recombinant Protein Expression-Molecular Cloning

University of Washington, Dr. Michael Holt
Seattle, WA
10.2022 - 12.2022
  • Amplified the DsRed gene using PCR and visualized the amplified product by agarose gel electrophoresis
  • Separated the DsRed gene protein of interest from the gel and Inserted the gene into the pET28a expression vector and introduced it into E Coli for amplification of the desired gene.

Isolation and Characterization of Lactate Dehydrogenase

University of Washington, Dr. Michael Holt
Seattle, WA
09.2022 - 11.2022
  • Utilized affinity chromatography, native gel electrophoresis, SDS-PAGE, and BCA analysis to enrich and isolate the LDH isozyme from porcine muscle homogenates
  • Investigated the distribution, activity, and purity of LDH, and analyzed its performance under various biological conditions to determine its kinetic constants and reaction mechanisms.

Immunological Investigation Through the IgY

University of Washington, Dr. Michael Holt
Seattle, WA
09.2022 - 11.2022
  • Enriched for antibody IgY in egg lysate using precipitation
  • Utilized various methods including microplate reader, blast quantification, SDS-PAGE, Western blot analysis alongside ELISA to demonstrate protein purity and quantity while investigating the mechanism of igY antibody.

Neuroengineering and Brain-Computer Interfaces

Prof. Dejan
UCLA
05.2022 - 07.2022
  • Participated in group discussions around neuro-engineering and brain computer interfaces
  • Collected and organized ideas of group members and integrated them into the research plan

Education

M.S in biotechnology - Molecular Targets & Drug Discovery Technologies

Johns Hopkins
Baltimore, MD
05-2025

Bachelor of Science - Biochemistry

University of Washington
Seattle
06-2023

Chongqing NanKai high School

Skills

  • PCR
  • Protein gel electrophoresis
  • Western Blot
  • SDS-PAGE
  • BCA Assay
  • Chromatography techniques
  • Spectroscopy and Spectrometry techniques
  • Native Mandarin speaker
  • Fluent in English

Languages

Native Mandarin speaker; Fluent in English

Timeline

Industrial R&D Intern

CanSino Biologics Inc
05.2023 - 08.2023

Recombinant Protein Expression-Molecular Cloning

University of Washington, Dr. Michael Holt
10.2022 - 12.2022

Isolation and Characterization of Lactate Dehydrogenase

University of Washington, Dr. Michael Holt
09.2022 - 11.2022

Immunological Investigation Through the IgY

University of Washington, Dr. Michael Holt
09.2022 - 11.2022

Neuroengineering and Brain-Computer Interfaces

Prof. Dejan
05.2022 - 07.2022

M.S in biotechnology - Molecular Targets & Drug Discovery Technologies

Johns Hopkins

Bachelor of Science - Biochemistry

University of Washington

Chongqing NanKai high School

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