Yuchen Zhao
Irvine,,California
Summary
Biotechnology Student Researcher trained to perform various lab skills. Work to make an impact in health industry and optimize personal values.
Overview
2
2
years of professional experience
Work History
Student Researcher
Razorenova Lab, University of California, Irvine
Irvine, California
09.2022 - Current
Synthetic lethality concept in cancer therapeutics
- Design and conduct 3 different experiments to investigate VHL-Rho protein interactions in 1 month
- Lead multiple weekly journal club each academic quarter to discuss papers with critical thinking skills
Student Researcher
Grainger Lab, San Diego State University
San Diego, California
10.2020 - 07.2021
Wnt, Stem Cells, Development and Cancer
- Master different cloning techniques (SLIC, Gibson Assembly, Restriction Cloning) and get trained on tissue culturing skills
- Design and construct 10 plasmids in 3 months
- Transfect HEK 293 T cells with the new plasmids to build new cell lines
- Feed, mate, and collect eggs from zebrafish, and perform genotyping using zebrafish tail
- Graph data from Western Blot and luciferase assay for publication
- DNA sample purification preparation for sequencing
Lab Assistant
Xi'an State Key Laboratory of Cancer Biology
Xi'an, Shaanxi, China
06.2019 - 09.2019
National Key Subject - Department of Physiology and Pathophysiology
- Train to sterilize and prepare laboratory equipment; equipped with Western Blotting and nucleic acid processing techniques in 1 month
Education
Master of Science - Biotechnology
University of California, Irvine
Irvine, CA06.2024
Bachelor of Science - Biology
San Diego State University
San Diego, CA05.2022
Skills
- Research Ability: Information Gathering and Organizing
- Biotechniques: Plasmid preparation, Western Blot, PCR, BCA assay, Molecular Cloning, Cell Passaging/Freezing and Thawing/Harvesting, Autoclaving, Nucleic Acid Extraction and Purification, Bacterial Culture Growing
- Equipment: ChemiDoc, Nanodrop, Spectrophotometer, Microscope, Autoclave, Bio-safety Cabinet
- Animal Work: Zebrafish
- Data Collection and Analysis: ENSEMBL, Galaxy, Benchling, ImageJ, BioTek Gen5, Image Lab Software, MS Word, Excel, PowerPoint
- Other: BioRender, Lab Procedures Tracking, Trouble-shooting, Graph editing for WB
- Bilingual: English & Chinese
Additional Scientific Project
Investigate CD93 ICD interaction with regulatory proteins and signaling pathway in leukemia stem cells
- Construct plasmid and purify CD93-ICD-GST protein to study protein interactions using pull-down assay
- Lead the team of 4 members to confirm shedding of CD93 from K562 cells and Metoclopramide blockage on the event
Interests
Weightlifting/Swimming/Rock-climbing/Nutrition/Travel
Timeline
Student Researcher
Razorenova Lab, University of California, Irvine
09.2022 - Current
Student Researcher
Grainger Lab, San Diego State University
10.2020 - 07.2021
Lab Assistant
Xi'an State Key Laboratory of Cancer Biology
06.2019 - 09.2019
Master of Science - Biotechnology
University of California, Irvine
Bachelor of Science - Biology
San Diego State University
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