Zhanna Bartosh

Accomplished Scientific Sales Representative and Scientist with a proven track record at AUM BioTech, LLC., enhancing gene regulation research and driving significant revenue growth through strategic sales and technical expertise. Expert in molecular biology and adept at solution selling, I excel in transforming complex scientific concepts into compelling product advantages, bolstering customer engagement and satisfaction.

• Tatiana Akimova, MD, PhD, Research Assistant Professor, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 916F ARC, 3615 Civic Ctr. Blvd, Philadelphia, PA, 19104, 215-200-1811, ttankmail@gmail.com
• Wayne W. Hancock, MD, PhD, FRCPA, Chief of the Division of Transplantation Immunology, The Children's Hospital of Philadelphia, Professor of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 916B ARC, 3615 Civic Ctr. Blvd, Philadelphia, PA, 19104, 215-590-8709, whancock@mail.med.upenn.edu
• Evgeniy Eruslanov, PhD, Research Associate Professor of Surgery, University of Pennsylvania, Department of Surgery, 3450 Hamilton Walk, Office 408A, Philadelphia, PA, 19104, 610-772-5624, evgeniy.eruslanov@pennmedicine.upenn.edu

"The Effect of Next-Generation FANA Antisense Oligonucleotides (ASO) on Human and Murine Tregs".

CD4+FOXP3 Regulatory T cells (Tregs) suppress immune response and control immune tolerance, but in tumors Tregs suppress anti-tumor immunity. Targeting Tregs is important part of anti-tumor therapy, in combination with other approaches or as a single therapy. FANA ASOs can self-deliver into tissues and are capable of highly specific and can achieve potent transient silencing of target mRNA.

• Process human tissues, including healthy and malignant lung tissue dissociation and isolation of PBMC from blood.
• Mouse tissues processing: prepare single cell suspension from tumors, livers, spleens, and lymph nodes for downstream applications.
• Immune cell isolation, including neutrophils and monocytes, using MACS.
• Cell culture and cryopreservation of cell lines and primary cells (PBMC and splenocytes) with various treatments.
• Proficient in RNA isolation from cells and tissues, cDNA conversion, and analysis by qRT-PCR.
• Skilled in flow cytometry, including running of flow cytometry panels, compensation with up to 12 colors, and the use of combined surface (lineage), intracellular (cytokine), and intranuclear (FOXP3) markers.
• Protein isolation and western blotting.

Tatiana Akimova, Liqing Wang, Zhanna Bartosh, Evgeniy Eruslanov, Steven Albelda, Sunil Singhal, Veenu Aishwarya,
Wayne W. Hancock, Targeting CD4+FOXP3+ Tregs to enhance anti-tumor immunity., 117th Annual Meeting of the
American Association for Cancer Research, San Diego, 04/01/24

doi: 10.3389/fimmu.2024.1426657. eCollection 2024.. 2024 Aug 21:15:1426657.Front Immunol

Tatiana Akimova 1 2, Liqing Wang 1, Zhanna Bartosh 1 2, Lanette M Christensen 1, Evgeniy Eruslanov 3, Sunil Singhal 3, Veenu Aishwarya 2, Wayne W Hancock 1

Affiliations expand

• PMID: 39234236
• PMCID: PMC11371716
• DOI: 10.3389/fimmu.2024.1426657