Zhuojin (Yvette) Wang

Project 1: Troponin Enhanceropathies: A Novel Role for the Troponin Genes.

• Conduct tissue histology by collecting the mice's organs from different gene lines
• Performed RNA extraction for different mice tissues, followed by reverse transcription to produce the cDNA.
• Conducted the qPCR for the cDNA and analyzed qualitative and quantitative data to examine the mRNA expression for different mice's organs.
• Generated the luciferase assay to determine the effect of the enhancer elements from human, rat, and mouse DNA, followed by bacterial transformation and DNA sequencing to check the insertion and purity of DNA.

Project 2: Characterizing Calmodulin (CaM) mRNA Subcellular Localization.

• Proposed to identify the localizations of CaM1, CaM2, and CaM3 mRNA in the mature cardiac myocytes by using cardiomyocyte isolation, primary culturing, pharmacological perturbations, and a hypertrophy mouse model.
• Cardiac myocyte isolation to obtain mice cardiac myocyte cell for RNA extraction.
• Performed the primary cell culture for heart fibroblast cells from both wild-type and mutant mice to investigate the localization of CaM in fibroblast cells.
• The experiments were conductedto test the binding specificity for CaM1 with potential baits.
• Analyzed the baits sequence by aligning with CaM1 gene sequence to find the pattern of clustering
• Conducted the optimization for RNA extraction to improve the yield from mice heart isolation for more efficient qPCR experiment.

Project: Antibody-Drug Conjugates (ADCs) to Treat Glioblastoma

• Proposed to investigate the treatment for glioblastoma by using antibody-drug conjugates and an intracranial xenograft model.
• Cultured engineered E. coli and extracted plasmids for plasmid transfection.
• Performed plasmid transfection of the chimeric anti-CD276 cell line for monoclonal antibody (mAbs) production.
• Purified ADCs using protein A columns, ion-exchange columns, G25 desalting columns, and dialysis.
  Evaluated ADCs for in vivo biodistribution using live-animal IVIS imaging.
• Adhered to Good Laboratory Practice (GLP) standards by implementing Standard Operating Procedures (SOPs) and maintaining accurate records.