Alane K. Seidel
Hershey
Summary
Results-driven scientific professional with robust background in laboratory management and focus on precision and efficiency. Brings history of successful leadership and operational effectiveness. Known for teamwork, adaptability, and fostering collaborative environments. Expertise in regulatory compliance and laboratory process optimization.
Overview
38
38
years of professional experience
Work History
Pediatric Oncology Translational Research Lab Manager
Pennsylvania State University, College of Medicine
10.2023 - Current
- Plans, organizes, and coordinates all the activities within the technical operations of the Translational Research Core Laboratory
- Leads and develops team members in the execution of the lab’s responsibilities
- Manages the Core Lab as an operational unit
- Coordinates with clinical research investigators and collaborators regarding collection and shipment of biological research materials and samples
- Manages ordering of laboratory supplies, chemicals and instrumentation
- Ensures laboratory safety and biosafety standards are maintained within the laboratory and that staff complete yearly chemical safety and biosafety training reviews
- Coordinates IACUC and IBC submissions, documentation and training for laboratory staff and research applications
- Performs daily laboratory duties including sample accessioning, cell culture and other biochemical assays
- Performs research experiments as directed including creating PDX models from patient tumors and cell lines
- Xenograft cancer models and testing drug treatment on mice
- Removing and processing tumors for further testing
- Participates in development and yearly review of laboratory SOP.s
- Maintained an organized inventory system for lab supplies, reducing waste and saving on procurement costs.
- Interviewed, hired and trained lab technicians to perform experiments and analyze results.
Core Facility Lab Manager
Pennsylvania State University, College of Medicine
01.1995 - 10.2023
- Manage the operations, administrative and laboratory procedures of the Transgenic Core Facility, through planning, organizing and delegating tasks for multiple complex research projects to fulfill the needs of clients’ fee-for-service projects
- Provide specialized service projects to Penn State & external researchers working as an expert individual contributor and manager for projects including planning, scheduling, implementing, recordkeeping and coordinating of the multiple phases of the projects in the following types of projects: Genetically modified animal production, Mouse rederivation, Colony management, Animal breeding, Cryopreservation, & Mouse line rescue
- Work with and establish relationships with internal and external parties in related fields to facilitate projects
- Write animal protocols for IACUC and IBC approval and ensure compliance with all regulations for each project
- Contribute to contract proposals, budget proposals and grant applications
- Prepare cost analysis of services, project cost estimates, and monthly billing reports
- Identify needs and add new services to the Core Facility: Mouse gametes cryopreservation services developed in 2014, expanded to gamete cryo-recovery services, started CRISPR microinjection services in 2015, and Mouse Colony Management services added in 2019
- Keep up with new technology and techniques in the field
- Supervise, evaluate performance of, and train fulltime research technologists in the lab
- Assign tasks, oversee completion of tasks, and provide guidance for duties that are assigned
- Coordinate ordering, maintain appropriate inventory and coordinate storage of supplies
- Maintain equipment and instruments essential to the operation of the lab
- Design, direct, and serve as lead instructor for the Mouse Colony Management courses
- Act as lead or assistant instructor on various rodent training classes and participate in the design and presentation of the training classes
- Act as guest instructor for Comparative Medicine residents’ Master-level classes CMED 503 and CMED 507
- Assist on workshops provided by the department
- Give presentations of work done by the Core to various audiences
- Serve on committees, including Comparative Medicine website committee, Strategic Planning, and SOP/Policy committee
- Attend meetings, work on departmental website as primary author of Core’s section, participate in departmental strategic planning, and write SOPs, guidelines and policies
- Fostered a positive working environment by promoting open communication among team members and addressing any conflicts promptly.
- Actively participated in professional conferences and workshops, sharing laboratory best practices and fostering a culture of continuous improvement.
Research Technician/Human IVF Lab
Pennsylvania State University, College of Medicine
03.1991 - 12.1994
- Perform techniques in human IVF, cryopreservation of human sperm and embryos, semen analysis, and the use of mouse embryo testing for all procedures and equipment
Research Technician
Pennsylvania State University, College of Medicine
11.1988 - 03.1991
- Perform techniques involved in research of Human papilloma virus, including cell culturing, southern blots, and mouse surgeries
Research Technician
Pennsylvania State University, College of Medicine
03.1988 - 11.1988
- Performed techniques involved in research of GABA receptors in rat brains
Research Assistant
George Washington University
01.1987 - 03.1988
- Performed techniques involved in research of the effect of proteins on lipid peroxidation of rat livers, including rat surgeries, HPLC, and cell culturing
Education
Bachelor of Science - Biology
Grove City College
Grove City, PA01.1986
Workshop on CRISPR, TALENS, and ZFNS. Bio Tech 55.
Foundation for Advanced Education in the Sciences/NIH
Bethesda, MD11.2015
Workshop on CRIPR-Cas Gene Targeting in Mice
Jackson Laboratory
Bar Harbor, ME11.2014
CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course
Roswell Park Cancer Institute
Buffalo, NY09.2014
Workshop on Colony Management: Principles and Practices
Jackson Laboratory
Bar Harbor, ME04.2009
Workshop on Cryopreservation of Mouse Germplasm
Jackson Laboratory
Bar Harbor, ME05.2013
Gene Targeting in ES Cells and Transgenic Mice
University of North Carolina at Chapel Hill
Chapel Hill, NC07.1996
Skills
- Standard operating procedures
- Lab equipment maintenance
- Sample processing
- Employee supervision
- Equipment management
Committee Memberships
- Department of Comparative Medicine SOP and Policy Committee, 01/01/09, 12/31/23
- Department of Comparative Medicine Website Committee, 01/01/09, 12/31/18
- Department of Comparative Medicine Strategic Planning Team, 01/01/14, 12/31/15
- Penn State Commission for Women Hershey Promotion and Marketing Committee, 01/01/16, 12/31/18
Affiliations
International Society for Transgenic Technologies, 01/01/11, Present
Papers Posters
- Efficacy of Select ALK Inhibitor Drugs on a Novel ALK Gene Mutation Found in a Pediatric Neuroblastoma Patient, Katherine McClain BS1, Jeremy Hengst PhD1, Alane Seidel BS1, Meenakshi Shukla BS1, Srinivasan Ekambaram, Giselle L. Saulnier-Sholler MD MSc1, Department of Pediatrics, Division of Hematology and Oncology, Penn State College of Medicine
- Pre-clinical evaluation of the selective sphingosine kinase 2 inhibitor, Opaganib, as a therapeutic strategy for treatment of rare pediatric cancers, Jeremy A. Hengst Ph.D1, Thussenthan Walter-Angelo BS1, Alane Seidel BS1, Katherine McClain BS1, Meenakshi Shukla BS1, Giselle Saulnier-Sholler MD1, Department of Pediatrics, Division of Hematology and Oncology, Penn State College of Medicine
- Evaluating the Efficacy of Targeted Therapies and Drug Combinations in Diffuse Intrinsic Pontine Glioma: Insights from Patient-Derived Cell Lines, Meenakshi Shukla MS1, Noah Kanis1, Divya Gandra MS1,2, Jeremy Hengst PhD1, Katherine McClain BS1, Alane Seidel BS1, Giselle Saulnier Sholler MD1,2, Department of Pediatrics, Division of Hematology and Oncology, Penn State College of Medicine
- Emergency Care of Orphaned Audiogenic EIII/JC-HLA-A2.1 Transgenic Rabbit Kits after Sudden Loss of All Nursing Does, Seidel, A., McClain K., Chang, C., Bryan, T., Peng, X., Department of Comparative Medicine, Penn State University College of Medicine, Hershey, PA
- The Use of Novel Materials and Artificial Mice for Genotyping and Identification Techniques Training, Osborne AJ., Seidel, A., McClain, K., Whitcomb, TL., Department of Comparative Medicine, Penn State University College of Medicine, Hershey, PA
- A Simple and Reliable Protocol for Induction of Superovulation in New Zealand White Rabbits, Peng, X., Seidel A.K., Department of Comparative Medicine, Penn State University College of Medicine, Hershey, PA
Workshops Courses
- Workshop on CRISPR, TALENS, and ZFNS, Foundation for Advanced Education in the Sciences/NIH, Bethesda, MD, 11/01/15
- Workshop on CRIPR-Cas Gene Targeting in Mice, Jackson Laboratory, Bar Harbor, ME, 11/01/14
- CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course, Roswell Park Cancer Institute, Buffalo, NY, 09/01/14
- Workshop on Cryopreservation of Mouse Germplasm, Jackson Laboratory, Bar Harbor, ME, 05/01/13
- Workshop on Colony Management: Principles and Practices, Jackson Laboratory, Bar Harbor, ME, 04/01/09
- Gene Targeting in ES Cells and Transgenic Mice, University of North Carolina at Chapel Hill, Chapel Hill, NC, 07/01/96
Timeline
Pediatric Oncology Translational Research Lab Manager
Pennsylvania State University, College of Medicine
10.2023 - Current
Core Facility Lab Manager
Pennsylvania State University, College of Medicine
01.1995 - 10.2023
Research Technician/Human IVF Lab
Pennsylvania State University, College of Medicine
03.1991 - 12.1994
Research Technician
Pennsylvania State University, College of Medicine
11.1988 - 03.1991
Research Technician
Pennsylvania State University, College of Medicine
03.1988 - 11.1988
Research Assistant
George Washington University
01.1987 - 03.1988
Workshop on CRIPR-Cas Gene Targeting in Mice
Jackson Laboratory
CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course
Roswell Park Cancer Institute
Workshop on Colony Management: Principles and Practices
Jackson Laboratory
Workshop on Cryopreservation of Mouse Germplasm
Jackson Laboratory
Gene Targeting in ES Cells and Transgenic Mice
University of North Carolina at Chapel Hill
Bachelor of Science - Biology
Grove City College
Workshop on CRISPR, TALENS, and ZFNS. Bio Tech 55.
Foundation for Advanced Education in the Sciences/NIH
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