Alyssa Barnes

Results-driven Research Associate with a strong molecular and cell biology foundation, such as protein purification, expression, and characterization, including SDS-PAGE, Western blotting, and large-scale plasmid preparations. Proven ability to execute cloning workflows and perform biophysical screening to support complex protein engineering projects. Adept in maintaining sterile lab conditions and preparing experimental stocks, buffers, and media with precision. Recognized for independently designing and analyzing experiments, contributing to SOP development, and enhancing workflow efficiency. Experienced in cross-functional collaboration with bioinformatics, technical services, and scientific teams to drive research innovation. Committed to continuous learning and contributing to high-impact drug discovery and biotechnology advancements.

• Research Assistant, "Molecular Experimentation on Microsporidia" (2024- Current) - Analyzed protein-protein interactions responsible for binding microsporidia to mitochondria.
• Master's Student Researcher, "Klebsiella aerogenes Respiratory Pathway Inhibition Role in Persister Cell Formation" (2023-2024) - Conducted experiments using respiratory inhibitors and respiratory mutants to identify key proteins in the respiratory pathway for potential drug targets to target persister cells specifically.

Targeting Respiratory Pathways to Combat Persistence: Investigating the Role of Respiratory Complexes in Klebsiella aerogenes Persister Cell FormationTargeting Respiratory Pathways to Combat Persistence: Investigating the Role of Respiratory Complexes in Klebsiella aerogenes Persister Cell Formation ProQuest · Dec 19, 2024

• Midwest Microbial Pathogenesis Conference, 2024 - "Impact of Respiratory Inhibitors on Persister Cell Formation in Klebsiella aerogenes: Insights from Modified Urinary Media"

• Midwest Enzyme Chemistry Conference - (2024)
• Midwest Microbial Pathogenesis Conference - (2024)
• Midwest Enzyme Chemistry Conference - (2023)

• Proficient in molecular biology techniques including SDS-PAGE, Western blotting, plasmid purification, and PCR for protein analysis and genetic manipulation.
• Skilled in bacterial culture management, colony isolation, and large-scale plasmid DNA preparation for molecular and microbiological research.
• Adept at maintaining aseptic lab conditions and preparing sterile stocks, buffers, and media to support reproducible experimental workflows.
• Experienced in executing and documenting laboratory protocols, ensuring compliance with SOPs, safety standards, and research integrity.
• Competent in analyzing experimental data and collaborating across multidisciplinary teams to support research in protein expression and functional screening.