Desired Job Title
Lab ManagerAcademic RepresentativeAcademic MentorCompliance AssociateCompliance ManagerWork Type
Full TimeLocation Preference
RemoteOn-SiteHybridLocation: Loveland, OHOrlando, FL
Open to relocation: Yes
Open To Work
Amanda Barboza
Loveland,OH
Work Preference
Summary
M.Sc. Neuroscience-Biology with research, leadership, and teaching experience that has worked on a high variety of research topics using many different techniques and model organisms. Comprehensive knowledge of neuroscience, toxicology, histology, anatomy, and physiology. I understand and can apply a large variety of research techniques including endpoint PCR, qPCR, RT-qPCR, ELISA, HPLC, Spectroscopy, DNA-sequencing, Western Blot, immunocytochemistry, and a large variety of histological and microscopy techniques including confocal microscopy, expansion microscopy, and super resolution techniques. 3 publications and 9 major presentations. Please look at my CV or Linkedin Profile for more details.
Overview
19
19
Certifications
10
10
years of professional experience
Work History
Bioimaging and Analysis Facility RAIII
Cincinnati Children's Hospital
04.2023 - Current
- Adapt to and understand various physiological, biological, and drug delivery projects brought to BAF by the varying scientists and their researchers at Cincinnati Children’s Hospital and assist/consult with those scientists on immunocytochemistry, histology, and imaging-based experiments.
- Performed lab work for the Bioimaging and analysis Facility including expansion microscopy and various clearing protocols.
- Tested new expansion and clearing techniques for staff scientists to troubleshoot and perfect SOPs/protocols.
- Trained cchmc staff Principal Investigators, scientists, researchers, and UC graduate students on the following:
- Software/hardware features and use of facility microscopes including but not limited to Nikon A1R/AxR, Yokogowa SoRa Spinning Disk, Multiphoton, Nikon AxR iLAS-TIRF, and AxR NSPARC systems.
- Use of IVIS live imaging system and how to preform proper luciferase imaging with necessary controls.
- Proper microscopy techniques, controls, and image validation.
- Proper image-based analysis including binary/threshold set up, 3D modeling structures, analysis validation, outlier tests, and control validation.
- How to train, validate, and use various AI analysis tools including Nikon Elements Object AI and IMARIS Machine Learning.
- Set up image analysis pipelines for scientist on various experiment types using Nikon Elements Advanced Research GA and GA3 (vs. 5.6 and 6.2) and IMARIS (vs 9, 10, and 11) software.
- Held bi-weekly “Introduction to Confocal Microscopy” course that covers basic confocal microscope mechanics, optics and light physics, sample preparation for microscopy, and Facility use.
- Maintained facility microscopes and objectives.
- Troubleshooting any issues with facility microscopes.
- Troubleshooting issues with user samples/sample preparation and offering advice.
- Maintained chemical inventory, OSHA Safety, and records for BAF.
- Prepare lesson plans and experiment SOPs for various imaging modalities and immunocytochemistry protocols.
- Scheduled and maintained the calendar of user sessions for consultations, trainings, and troubleshooting.
- Maintained contact with and assisted users on imaging experiments from set-up through analysis.
Lead Lab Technician
Miami University
01.2022 - 01.2023
- Supervised daily laboratory operations, ensuring compliance with safety protocols and quality standards.
- Trained and mentored junior technicians on best practices and equipment handling procedures.
- Developed and implemented workflow improvements to enhance efficiency in lab processes.
- Coordinated sample collection, preparation, and analysis for research projects across various disciplines.
- Performed any necessary lab techniques for the Microbiology department including but not limited to DNA extraction and purification, spectrophotometry (Nanodrop and Qubit), PCR, endpoint PCR, gel electrophoresis, qPCR, and DNA library preparation for Illumina Sequencing.
- Helped maintain the Wilson Lab Mice colony including conducting a biweekly census and earmarking.
- Helped maintain the cleanliness of the Animal Care Facility surgery rooms to aseptic standards.
- Helped write and prepare lesson plans and experiment SOPs for a PCR workshop.
- Trained and supervised other Lab Techs to perform DNA extraction, purification, PCR techniques, and gel electrophoresis.
- Wrote SOPs for DNA extraction, purification, PCR techniques, and gel electrophoresis.
- Helped maintain the microbiology classrooms, including cleaning the lab space, keeping supplies stocked for the students, and preparing solutions including gram stain solutions and different bacterial growth media.
Lead Lab Tech-Infectious Disease Surveillance
Miami University
11.2021 - 12.2022
- Performed experiments to optimize the testing protocol and evaluate the feasibility and efficacy of alternative probes and enzyme sources.
- Oversee laboratory operations to ensure compliance with safety and quality standards, including hazardous waste disposal, chemical safety and OSHA.
- Manage inventory of laboratory supplies, optimizing procurement processes for efficiency.
- Mentor junior technicians on best practices and adherence to protocols.
- Implement new testing procedures to enhance accuracy and reliability of results.
- Prepared and ran PCR tests on saliva samples to detect the prevalence of COVID19 among the student population.
- Performed experiments to optimize the testing protocol and evaluate the feasibility and efficacy of alternative probes and enzyme sources.
- Organized and analyzed data regarding COVID19 infection on a college campus, including data protected by HIPPA
- Helped produce and edit a scientific manuscript (paper in review) and SOPs.
- Trained and supervised BS level Lab Technicians to perform COVID19 testing procedure
- Organized and kept track of laboratory inventory.
- Maintained laboratory and equipment up to BSL2 standards
- Conduct regular equipment maintenance, minimizing downtime and ensuring operational readiness.
- Oversaw safe handling and storage of hazardous materials in compliance with regulations.
- Trained staff on proper safety protocols and emergency response procedures for hazardous material incidents.
- Conducted regular inspections of hazardous material containers to ensure integrity and compliance with safety standards.
- Developed and implemented improved workflows for hazardous waste disposal, enhancing operational efficiency.
Research Assistant
Florida Atlantic University
08.2019 - 08.2020
- Conducted literature reviews to support research projects and identify relevant studies.
- Analyzed data sets using statistical software to derive insights and inform project direction.
- Managed laboratory equipment and resources, ensuring proper maintenance and optimal functionality.
- Analyzed complex data sets to uncover critical insights, driving direction of research projects.
- Collaborated with faculty on grant proposals, enhancing funding opportunities for research initiatives.
- Created and maintained comprehensive databases for tracking research progress and outcomes.
- Trained junior researchers on methodologies, fostering a collaborative team environment.
- Developed experimental protocols, ensuring adherence to ethical guidelines and best practices.
- Led weekly research meetings, facilitating discussions on project developments and strategic planning efforts.
- Participated actively in regular meetings with fellow researchers to discuss project updates, challenges faced, and lessons learned during ongoing activities.
- Collaborated with other researchers in various laboratories on a multitude of different projects (See below).
- Toll Lab: Performed experiment to gather preliminary data on the distribution of specific opioid receptors in UDISCO cleared skin (mice). Assisted in gathering data on the distribution of opioid receptors in the trigeminal ganglion of migraine model mice (paper published). Organized and maintained laboratory equipment. Utilized Aseptic technique.
- Guthrie Lab: Gathered data on the location of UBE3/NeuN colocalized interneurons in the olfactory bulb to explore neural regeneration in the olfactory bulb, learned to genotype mice. Organized and maintained laboratory equipment. Utilized Aseptic technique.
- Godswedge lab: Sorted and crossed flies with desired genetics to produce UAS/GAL4 flies with specific mutations. Assisted with research on axonal transport.
- Expert in Confocal microscopy
- Conducted hazardous materials assessments to identify and mitigate potential risks.
- Managed disposal of hazardous waste in compliance with environmental regulations.
- Trained staff on safety protocols for handling hazardous substances.
- Oversaw inventory management of hazardous materials, ensuring proper labeling and storage.
Graduate Research Assistant
Miami University
08.2016 - 08.2019
- Thesis Research: Utilized a custom clearing technique and immunocytochemistry to determine the distribution of a neuromodulator within the crustacean CNS (paper published and a second in process). Thesis research was conducted independently and with an undergraduate (Hailey King) who I trained and supervised.
- Learned and utilized confocal microscopy, immunocytochemistry, various histochemical techniques, solution preparation including dilutions and titration, basic laboratory maintenance, clearing techniques, hazardous material use, and waste disposal.
- Mentored undergraduate and incoming graduate students, providing guidance on research methodologies and techniques and assisting with papers, presentation (undergraduate research forum) and posters.
- Itineraries and Departmental Progress reports are available upon request.
- Reviewed technical and professional publications and journals to stay current on recent literature and make more strategic research decisions.
- Followed strict safety protocols, protecting staff and assets while carrying out technical procedures and research protocols.
- Conducted literature reviews to support research projects and identify gaps in existing studies.
- Collaborated with faculty and graduate students on interdisciplinary research initiatives.
- Presented research findings at academic conferences, enhancing visibility of departmental work.
- Coordinated project timelines and deliverables, ensuring alignment with research objectives and deadlines.
- Utilized advanced statistical software to analyze large datasets, uncovering key insights for informed decisionmaking.
- Managed multiple research projects simultaneously, ensuring timely completion and adherence to high-quality standards.
- Contributed to professionally-written scientific paper for publication and first authored a paper
Graduate Teaching Associate
Miami University
01.2017 - 05.2019
- TA Human Physiology Lab for non-biology majors and biology majors, and Human Anatomy Lab for biology majors and premedical students. Human Anatomy Head TA Fall 2018 and Spring 2019. Two, 2-hour sections of up to 40 students per week.
- Prepared and gave a 30-minute plus lecture before each lab suited to non-biology majors, majors, and pre-medical students.
- Explained, aided, and guided students through experiments and dissections to understand human physiology and/or anatomy at fundamental and advanced levels.
- Facilitated engaging discussions to enhance student understanding of complex concepts.
- Organized, prepared, and graded worksheets, exams, and class materials.
- Coordinated up to 6 different sections of Human Anatomy. Led Teaching Assistants through each laboratory exercise and explained any necessary material during weekly lab meetings.
- Developed and implemented inclusive lesson plans tailored to diverse learning styles.
- Kept Teaching Assistants updated on the newest information, examples, and updating materials.
- Handled any issues regarding Teaching Assistant or student behavior promptly and to the best of my ability.
- Evaluated student performance through assessments, providing constructive feedback for improvement.
- Collaborated with faculty to refine curriculum and improve educational outcomes across courses.
- Mentored undergraduate students, fostering academic growth and professional development skills.
- Organized workshops focusing on research methodologies and effective study strategies for students.
- Enhanced student comprehension by incorporating diverse teaching methods and strategies.
- Developed strong rapport with students, fostering a positive learning environment and promoting academic achievement.
- Mentored new Graduate Teaching Associates, sharing best practices for effective teaching and classroom management.
- Implemented innovative classroom management techniques, maintaining order and facilitating focused learning.
- Served as a liaison between faculty and students, addressing concerns and facilitating communication for improved academic outcomes.
- Assisted with development and implementation of new course materials to enhance learning experience.
- Documented attendance and completed assignments to maintain full class and student records.
- Monitored student progress to identify weaknesses and provide recommendations for improvement.
- Mentored and advised students to focus on academics and explore career paths and opportunities.
- Prepared letters of recommendation for students looking to obtain internships and cooperative opportunities.
Education
Master of Science - Biology
Miami University
Oxford, OH08-2019
Skills
- Toxic chemical inventory record keeping, safety, and laboratory use (TTx, alphabungero toxin, other chemicals used in neuroscience/opioid research)
- Scheduled Narcotic drug-handling, records, and safety (Pentobarbital and various Opioids)
- Hazardous waste/chemical determination, storage, and disposal
- BL2/Human sample laboratory maintenance, waste disposal and protocols
- Basic lab techniques including solution preparation, dilutions, titrations, stains, and preparation of different types of bacterial media, animal model injections/dosing, SOP design and following SOPs, Hazardous substance handling, record keeping
- Experimental design/planning
- Scientific writing including Specific Aims, Proposal writing, and SOPs
- PCR (endpoint, multiplex qPCR) and gel electrophoresis
- DNA/RNA extraction and purification
- Western Blot
- Spectroscopy
- Confocal and super resolution microscopy
- Immunocytochemistry (including antibody preparation and solution/buffer preparation) and ELISA
- Tissue clearing and expansion techniques
- Histochemical techniques including methylene blue, fast dyes, and dye filling cut nerves
- Aseptic technique
- Tissue dissection, fixation, and storage
- Data Analysis/Scientific Analysis and writing
Transferable skills
- Critical thinking
- Classroom management and leadership/Mentorship
- Laboratory Supervision
- Laboratory maintenance (including keeping inventory/stock)
- Communication/Presentation
- Schematic and model drawing/production
- Experimental design
- Friendly, positive attitude
- Teamwork and collaboration
- Customer service
- Problem-solving
- Time management
- Attention to detail
- Flexible and adaptable
- Organization and time management
- Decision-making
- Verbal communication
Other skills:
Affiliations
- Society for Neuroscience, Member (2016-2022)
- Nu Rho Psi-Gamma in Ohio: Neuroscience Honor Society, Member (2016-2019)
- NSO: Neuroscience Student Organization, Member (2019-2020)
Certification
Certifications via CITI training
- Humanities Responsible Conduct of Research
- Using Animal Subjects in Research (RCR basic)
- OSHA Blood Born Pathogen
- Biosafety and Security
- CITI Information Privacy and Security (IPS) for Clinical Investigators
- Human Research-Data or Specimens Only Research
- Mandatory General Training for All Animal Useres
- Reducing Pain and Distress in Laboratory Mice and Rats
- Working with Mice in research Settings
- Working with Rats in Research Settings
- Aseptic Surgery (Animals)
- Responsible Conduct of Research
- Basic Intro to Biosafety
- Working with Genetically Modified mice in research settings
- Working with IACUC
- IACUC Community Member
- Shipping and Transport of Regulated Biological Materials
- Biosafety Officer Training
- Personal Protective Equipment
Timeline
Bioimaging and Analysis Facility RAIII
Cincinnati Children's Hospital
04.2023 - Current
Lead Lab Technician
Miami University
01.2022 - 01.2023
Lead Lab Tech-Infectious Disease Surveillance
Miami University
11.2021 - 12.2022
Research Assistant
Florida Atlantic University
08.2019 - 08.2020
Graduate Teaching Associate
Miami University
01.2017 - 05.2019
Graduate Research Assistant
Miami University
08.2016 - 08.2019
Master of Science - Biology
Miami University