Amy Funk
Minneapolis,MN
Summary
Dedicated and adaptable professional with a proactive attitude and the ability to learn quickly. Strong work ethic and effective communication skills. Eager to contribute to a dynamic team and support organizational goals.
Overview
1
1
Certification
Work History
Lab Technician
Mycoplasma Testing Lab- WuXi AppTec
- Current
- Performing mycoplasma detection assays utilizing techniques such as observation of broth cultures and agar plates, sub-passage of mammalian cell cultures, fixing and staining of cell cultures, and fluorescent microscopy
- Maintain aseptic technique, adequate gowning, and perform environmental monitoring in a cleanroom environment.
- Perform validation testing of media and reagents, including contamination checks and growth promotion testing.
- Performed regular inspections on all equipment to ensure proper functioning.
- Prepare and perform dilutions of biological test samples prior to inoculation.
- Track inventory and maintain positive control, master, and working stocks.
- Track media inventory and coordinate media production
- Preparation and documentation of testing procedures using Good Documentation Practices (GDP)
- Assist in reviewing and revising Standard Operating Procedures (SOPs) and forms.
- Performed quality control tests on incoming and outgoing materials to ensure safety standards were met.
- Followed strict safety protocols while handling hazardous materials in the laboratory environment.
Lab Assistant
Microbiology Media Prep- Merieux NutriSciences
08.2019
- Implemented an organization system of media source stock intake and storage
- Experience with XY machines, peristaltic pumps, pH meters, autoclaves
- Preparation of media according to SOPs
- Quality Control set up and inoculation
- Maintained laboratory equipment and supplies, ensuring proper functioning of instruments.
- Calibrated instruments according to manufacturer specifications.
- Provided technical guidance to junior staff members regarding laboratory procedures and protocols.
- Sanitized workstations and instruments to avoid cross-contamination.
Education
Masters of Genomics -
University of The Sciences
Philadelphia, PA12-2024
Bachelor of Arts - Biology -
Metropolitan State University
St Paul, MN12.2019
Associate of Arts -
Ridgewater College
Willmar, MN12.2010
Skills
- Specimen handling
- Supply Management
- Laboratory safety procedures
- Specimen Processing
- Test Reporting
- Laboratory Testing
- Chemical Reagent Preparation
- Culture Maintenance
- Regulatory Compliance
- Specimen Preparation
- Document processing
- Sterile technique
- Laboratory safety protocols
Independent Research
Metropolitan State University, Bacteriophages were isolated from environmental filtrates and screened against 12 BSL1 bacteria using a spot assay. Raw sewage showed the most promising results with forming plaques on the E. coli K12 plate. Other strains of E. coli (EC-B and EC-C) were cultured and used to further specify the host range of the phage. After inoculation and incubation, the cultures were serial diluted (10^1-10^10), and all sequential dilutions were plated with their corresponding E. coli strain. EC K12 showed the most substantial results on dilutions 101 and 102. Plaques were removed from these plates and incubated in cultures of EC K12. The most evident result was in culture (#3), which indicated high levels of lysis via complete clearing of the culture’s turbidity. Samples from culture #3 were used for a spot assay against the 12 BSL1 bacteria. The only plate with plaque formation was EC k12., This Phage is currently being used in the lab portion of the Microbiology course at Metropolitan State University to type E. coli. The phage has not been sequenced and is currently named Funka3.
Relatedcoursework
- Biology of Microorganisms
- Biology of Microorganisms Laboratory
- Molecular Biology
- General Chemistry I & II
- General Chemistry Laboratory I & II
- Organic Chemistry I
- Organic Chemistry Laboratory I
- Phage Hunting to Phage Typing
- General Biology I & II
- General Biology Laboratory I & II
- Cell Biology & Histology
- Principles of Genetics
- Calculus I, Calculus II
- Statistics I
- Ecosystem & Global Ecology
- Intro to Physics
- Evolution
- Seminars in Biology
- Animal Diversity
- Geology and Earth Systems
Certification
- Graduate Certificate: Genetic Counseling
Timeline
Lab Assistant
Microbiology Media Prep- Merieux NutriSciences
08.2019
Lab Technician
Mycoplasma Testing Lab- WuXi AppTec
- Current
- Graduate Certificate: Genetic Counseling
Masters of Genomics -
University of The Sciences
Bachelor of Arts - Biology -
Metropolitan State University
Associate of Arts -
Ridgewater College
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