ANA CURI LOPES
Deland,USA
Summary
Dedicated and skilled research technician with extensive laboratory experience in molecular biology, cell culture, and genetic research. Proficient in techniques like organoids, PCR, CRISPR, RNA-seq, flow cytometry, and ELISA, as well as tissue culture and protein extraction. Demonstrated ability to support complex experiments, manage and analyze data sets, and maintain accurate documentation. Experienced in collaborating with research teams to assess gene fitness, monitor gene expression, and investigate tumor microenvironments. Strong organizational skills and proficiency with data analysis software, ensuring efficient and accurate experimental outcomes.
Overview
4
4
years of professional experience
Work History
Student Researcher
Stetson University
01.2024 - Current
- Developed and conducted a research project in the field of biochemistry.
- Improved the use of 2D NMR for probing distances in proteins and studying protein structure.
- Cultured cell lines, induced mutations, and extracted and purified proteins.
- 1D and 2D NMR.
- Mastered the use of paramagnetic metals for probing distances in proteins.
Student Intern
Stetson University
08.2023 - 12.2023
- Assisted Dr. Lynch in researching the physics of tissue migration.
- Cultured and extracted tissue from axolotls.
- Prepared the tissues for computational analysis.
- Used Fiji to quantify tissue migration.
- Documented findings and kept data organized.
Peer Lead
Stetson University
01.2023 - 12.2023
Student Intern
Boston Children's Hospital
05.2023 - 08.2023
- Assisted scientists in the Breault Lab.
- Performed behavioral and physiological assessments to monitor health and tumor progression in animal models, and subsequently removed, digest, and cultured adrenal tumors to develop cell lines for in-vivo and In-vitro experiments.
- Conducted flow cytometry experiments for sorting and assessing tumor immune infiltrates, and studied tumor microenvironment dynamics, including immune cell infiltration and cell-cell interactions.
- CRISPR screen experiments, involving cell line cloning for specific gene knockout and in vivo subcutaneous implantation, while investigating the effects of immune checkpoint inhibitors on tumor growth.
- Utilized ELISA to measure steroid hormones, and renin levels in mice and cell lines.
- Performed qPCR, RNAScope, and immunofluorescence
- Utilized Photoshop and Fiji to quantify adrenal cell images.
- Developed Organoids culture techniques to study immune system- tumor interactions in adrenal cortex carcinoma.
- Conducted PCR and gel electrophoresis to maintain and genotype mouse colony.
Clerical Assistant
Stetson University Library
08.2022 - 12.2022
- Greeted incoming visitors and customers professionally and provided friendly, knowledgeable assistance
- Maintained organized files and stocked supplies to support team needs and maximize performance
- Kept the books organized in the library and helped visitors check out books and equipment they needed
- Provided support to costumers giving advice on books and equipment they should look at for their specific goals
Student Intern
Boston College
05.2022 - 08.2022
- Assisted scientists at the van Opjinen Lab.
- Induced targeted mutations in bacterial genomes to study gene essentiality.
- Cultured and maintained various cell lines, ensuring proper growth conditions.
- Performed insertion sequencing for genetic screening.
- Performed PCR for gene amplification and verification of genetic modifications.
- Conducted protein extraction and purification to support downstream analyses.
- Prepared RNA samples and performed RNA-seq to monitor gene expression profiles.
- Managed and analyzed data sets, contributing to the identification of universal and strain-dependent essential genes.
- Supported the assessment of gene fitness through statistical analysis of experimental data.
- Documented findings.
Front Desk Manager
Brazil Academy
01.2021 - 08.2021
- Greeted visitors and customers upon arrival, offered assistance, and answered questions to build rapport and retention
- Mentored and trained new hires, fostering a supportive work environment that facilitated professional growth
- Responded to telephone, email and in-person inquiries regarding classes and prices
- Signed up costumers to classes and sold the program to people who showed interest either in person, online, or over the telephone
Education
Bachelor of Science - Biochemistry
Stetson University
05.2025
Skills
- Immunofluorescence
- Mice
- Organoids
- Western Blot
- Cell culture
- Microscope
- Python
- Adobe
- Database entry
- Administrative Support
Languages
English
Native or Bilingual
Portuguese
Native or Bilingual
Spanish
Full Professional
Timeline
Student Researcher
Stetson University
01.2024 - Current
Student Intern
Stetson University
08.2023 - 12.2023
Student Intern
Boston Children's Hospital
05.2023 - 08.2023
Peer Lead
Stetson University
01.2023 - 12.2023
Clerical Assistant
Stetson University Library
08.2022 - 12.2022
Student Intern
Boston College
05.2022 - 08.2022
Front Desk Manager
Brazil Academy
01.2021 - 08.2021
Bachelor of Science - Biochemistry
Stetson University