Anamika Sharma
Detroit,MI
Summary
A highly motivated and detail-oriented recent graduate with a strong foundation in biotechnology and molecular biology, seeking an entry-level position in the biotech industry to apply my academic knowledge and research skills in a dynamic and innovative environment.
Overview
8
8
years of professional experience
Work History
Graduate Research Assistant
Wayne State University School of Medicine
09.2021 - Current
- Investigated the role of microglia in pathological angiogenesis.
- Described IL-33/ST2 signaling on microglia activation and its impact on retinal neovascularization.
- Standardized the technique for mice retina cell isolation, coculture microglia and endothelial cells, sprouting, cell migration assays.
- Performed confocal microscopy to access activated microglia around retinal pathological tufts, retina whole mount imaging, Z stacking in confocal microscopy.
- Work collaboratively with lab members to manage lab and animal housing.
- Perform disscection of mice to collect the organs.
- Analyzed experimental data using GraphPad Prism, SPSS, and ImageJ for quantification and statistical validation.
- Contributed to manuscript writing, figure preparation, and literature reviews for peer-reviewed publications
- Prepared histological slides and conducted staining (H&E, IHC, IF) for structural tissue analysis.
- Collaborated with interdisciplinary teams on FACS analysis.
Masters Student
DUVASU
08.2017 - 09.2019
- Studied prenatal development of thyroid gland in goats.
- Observed histological changes and development of fibers, follicles, and blood vessels.
- Performed the dissection in goat fetus to isolate the thyroid glands.
- Performed the paraffin sectioning of fetus from 31 days to 141 days old.
- Performed H and E staining, PAS staining, Mason's Trichrome stain, Verhoeff stain, Sudan Black stain, Alcian blue stain.
- Provided instructions in histological techniques and microscopy to undergraduate students.
Education
Ph.D. - Anatomy & Cell Biology
Wayne State University
Detroit, Michigan08-2025
M.V.Sc. - Veterinary Anatomy
DUVASU
INDIA09-2019
B.V.Sc & A.H. -
GADVASU
INDIA07-2017
Skills
Molecular Biology: DNA/RNA isolation, PCR, RT-PCR, Real-time PCR, Agarose gel electrophoresis, ELISA, Western blotting, Immunohistochemistry, Immuno-fluorescence, gelatin zymography
Lab Animal Handling: Collection of eyes from mice, Retina isolation, Sclera isolation, Flat mount preparation, Dissection of mice
Microscopy: Fluorescent microscopy, Confocal Microscopy, Phase contrast microscopy, Live-cell imaging and time-lapse microscopy, Cryosectioning and microscopy of frozen tissues, Quantitative image analysis and morphometric assessments, Histological slide preparation and staining (H&E, IHC, IF), Immunofluorescence staining and imaging
Cell Culture: Maintenance of cell lines, primary cell culture, transfection, sprouting, migration, tube formation, endothelial cell dysfunction assays, 3D cell culture, co-culture systems, cryopreservation, cell viability assays, proliferation assays, differentiation protocols, and contamination control
Bioinformatics & Data Analysis: Basic knowledge of databases and analysis tools like BLAST, NCBI, UniProt, Ensembl, SPSS, Graph-pad (Prism)
Software Skills: Microsoft Word, PowerPoint, Excel, Adobe Illustrator, Photoshop, ImageJ
Interpersonal Skills: Attention to detail, Verbal communication, Critical thinking, Organization and time management, Analytical thinking, Quantitative analysis, Teamwork, Quick learner
Accomplishments
- Received ARVO Foundation Travel Grant for ARVO meeting 2025.
- Delivered poster presentation on Role of IL-33 induced microglia activation on pathological angiogenesis at Vision Research Workshop, Wayne State University, 2024.
- Delivered poster presentation on Adam10 Regulates Ephrin B2-Mediated Endothelial Cell Sprouting and Ischemic Retinopathy at Michigan Physiological Society Annual Meeting, 2023.
Languages
English
Full Professional
Hindi
Native or Bilingual
Publications
Bisen, S., Gogoi, P., Sharma, A., Mukhopadhyay, C. S., & Singh, N. K. (2025). ADAM10 regulates EfnB2-mediated Endothelial Cell Sprouting and Ischemic Retinopathy. The American Journal of Pathology.
Sharma, A., Gupta, D. K., Bisen, S., & Singh, N. K. (2024). Comparative evaluation of trypsin and elastase digestion techniques for isolation of murine retinal vasculature. Microvascular Research, 154, 104682.
Sharma, A., & Singh, N. K. (2023). Long non-coding RNAs and proliferative retinal diseases. Pharmaceutics, 15(5), 1454.
Sharma, D., Kaur, G., Bisen, S., Sharma, A., Ibrahim, A. S., & Singh, N. K. (2023). IL-33 via PKCμ/PRKD1 mediated α-catenin phosphorylation regulates endothelial cell-barrier integrity and ischemia-induced vascular leakage. Cells, 12(5), 703.
Timeline
Graduate Research Assistant
Wayne State University School of Medicine
09.2021 - Current
Masters Student
DUVASU
08.2017 - 09.2019
Ph.D. - Anatomy & Cell Biology
Wayne State University
M.V.Sc. - Veterinary Anatomy
DUVASU
B.V.Sc & A.H. -
GADVASU
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