ANJANA SANTOSH KUMAR

• Managed biorepository operations, including organization, retrieval, and storage of protein samples across multiple research studies.

• Maintained detailed records of sample locations and metadata using Microsoft Excel, streamlining inventory tracking.

• Handled REDCap data exports, conducted preliminary data analyses, and supported summary report generation for research documentation and compliance.

• Assisted in analyzing cognitive function data in older adults, exploring patterns in cognitive variability.

• Ensured proper handling and storage of biological specimens to preserve sample integrity.

• Supported lab operations, streamlined workflows, and managed administrative tasks to facilitate smooth research execution.

• Conducted differential gene expression analysis for Huntington's disease (HD) Q111 mouse model using Pseudobulk analysis by Edge R, identifying molecular changes linked to neurodegeneration, cellular stress, and synaptic activity.
• Performed cell type-specific analysis of neuronal and glial subtypes with top marker genes, ensuring accurate classification of cellular populations.
• Generated UMAP dot plots, heatmaps, and volcano plots to visualize and communicate cell type-specific gene expression patterns by seurat.
• Designed and implemented data interpretation strategies to uncover cellular and molecular mechanisms underlying neurodegeneration.
• Did GSEA on DEGs to verify the genes we found in our data set.
• Identified and resolved data issues to drive accuracy and integrity.

• Gained practical experience in AAV production, vector construction, CRISPR-based gene editing, gene therapy methodologies, and aseptic cell culture techniques.
• Engineered plasmid vectors, produced and quantified AAV vectors, and executed CRISPR-based gene knockouts, base editing, and prime editing.
• Cleaned and prepared lab equipment for solutions, specimens and samples.
• Supervised supplies in inventory and notified supervisor of low stock levels.
• Kept lab space and equipment clean to prevent contamination with hazardous substances.

• Provided first aid and CPR support to ensure the safety and well-being of facility patrons.
• Communicated effectively with members to address inquiries, provide guidance and promote fitness programs.
• Assisted in managing team operations to maintain a clean, organized, and safe workout environment.
• Responded promptly to emergencies, adhering to established safety protocols and procedures.

• Synthesized and characterized Sorafenib-loaded PLGA nanoparticles for cancer drug delivery applications.

• Decorated nanoparticles with TKD peptide for active targeting; confirmed peptide coupling via FTIR.

• Gained expertise in solid-phase peptide synthesis (SPPS) and evaluation of nanoparticle drug loading efficiency.

• Developed biocompatible calcium carbonate (CaCO₃) nanoparticles synthesized from eggshells for sustainable drug delivery.

• Characterized nanoparticles for drug loading efficiency and release kinetics to optimize therapeutic potential.

• Contributed to research on nanotechnology applications in pharmaceutical sciences.