ARJUMAN LIMA
Normal,USA
Summary
Dynamic plant molecular biologist and genetic engineer specializing in CRISPR/Cas9 gene editing and advanced transformation techniques. Skilled in plasmid preparation, sequencing analysis, and primer optimization. Strong technical abilities in plant tissue culture, DNA/RNA purification, and particle bombardment. Proficient in bioinformatics tools, data interpretation, and statistical analysis. Demonstrated success in collaborative research and laboratory leadership. Known for meticulous attention to detail and commitment to advancing crop development for sustainable agriculture.
Overview
8
8
years of professional experience
Work History
Graduate Research Assistant
Illinois State University
08.2022 - Current
- Design and execute CRISPR/Cas9-based gene editing experiments for crop improvement.
- Conduct Agrobacterium-mediated transformation for gene insertion in plant cells.
- Generate plasmid preps, perform restriction enzyme (RE) digestion, and validate constructs via sequencing.
- Design, optimize, and troubleshoot primers for PCR applications.
- Perform DNA/RNA extraction, purification, PCR, and sequencing analysis to assess gene integration and expression.
- Apply tissue culture techniques including callus induction, regeneration, and hormone treatments.
- Optimize media preparation to support efficient plant tissue culture growth.
- Conduct particle bombardment-mediated transformation for DNA delivery in plant tissues.
- Utilize bioinformatics tools (e.g., BLAST, Galaxy, Geneious, NCBI databases) for sequence analysis.
- Perform statistical analysis using software like SPSS and R to interpret experimental data.
Research Assistant
Chattogram Veterinary and Animal Sciences University
12.2018 - 07.2022
- Sample collection from animal, DNA extraction, PCR, qPCR
- Genomic selection methods and Marker-assisted selection for animal breeding programs
- Skilled in identifying and analyzing single nucleotide polymorphisms (SNPs)
- Interpret by genomic analysis to make informed breeding decisions
- Growing, isolating, and characterizing microorganisms in various environments
- Collaborated with research team for successful completion of various microbiology projects, resulting in valuable insights and discoveries.
Veterinary Medicine Internship Program
01.2017 - 12.2017
- Acquired skills in laboratory diagnostics, such as bloodwork, urinalysis, and microbiological testing, which are critical for diagnosing animal diseases
- Learned how to document health assessments, treatment plans, and outcomes accurately for legal and medical purposes
Education
Master's Student - Biotechnology Major
Illinois State University
05.2025
Master of Science - Animal Breeding and Genetics
Chattogram Veterinary and Animal Sciences University
12.2020
Bachelor - Doctor of Veterinary Medicine
Chattogram Veterinary and Animal Sciences University
12.2016
Skills
- CRISPR/Cas9 Gene Editing
- Molecular Cloning
- Agrobacterium-mediated transformation
- Primer design and optimization
- Plant tissue culture and transformation techniques
- DNA/RNA Extraction and Purification
- Culturing, isolating, and identifying microorganisms
- Genomic sequencing analysis
- Bioinformatics
- Confocal and Electronic Microscopy
- Statistical analysis
- HPLC analysis
- Skilled in using SNP markers
Timeline
Graduate Research Assistant
Illinois State University
08.2022 - Current
Research Assistant
Chattogram Veterinary and Animal Sciences University
12.2018 - 07.2022
Veterinary Medicine Internship Program
01.2017 - 12.2017
Master of Science - Animal Breeding and Genetics
Chattogram Veterinary and Animal Sciences University
Bachelor - Doctor of Veterinary Medicine
Chattogram Veterinary and Animal Sciences University
Master's Student - Biotechnology Major
Illinois State University