Ashutosh Yende
Bethesda
Summary
Immuno-oncologist specializing in multicolor flow cytometry, organoid culture, and immunotherapy. Proven track record in managing in vivo mouse models and genomics data analysis platforms. Extensive experience in leading projects and fostering collaborative efforts within research teams.
Overview
14
14
years of professional experience
Work History
Scientist
Henry Jackson Foundation for the Advancement of Military Medicine
Bethesda
02.2025 - Current
- Project Manager and lab-lead managing 4 ongoing projects in Dr. Symes' lab, actively managing teams comprising postdocs, research trainees and lab technologists.
- Developing 3D brain scaffolds to model brain tissue architecture, serving as molecular biology, flow cytometry and in vivo lead on multi-institute collaborative projects.
Postdoctoral Fellow
Johns Hopkins Medicine
Baltimore
07.2022 - 01.2025
- Multicolor (12+) flow cytometry panel design, optimization, and data acquisition.
- Expertise on BD LSRII, LSRFortessa, Celesta systems and FACS Diva, FCS Express software. Trained on Sony SH800 cell sorter.
- T cell function, myeloid subpopulation, general immunophenotyping and stemness markers panel design, analysis to identify tiniest of populations.
- Leading 2 projects focused on cancer drug development and cancer immunotherapy in mice models.
- Testing anti-PD1 immunotherapy efficacy and gut microbiota association in in-house developed syngeneic obese breast cancer mouse models.
- Tumor dissociation, isolation and culture of primary cells.
- Multiple xenograft studies to test and develop anticancer drugs candidates.
- Routinely performed IP injections, monitored tumor growth, drug toxicity assessment.
- Identified 5 tumor suppressors and 3 oncogenes by performing RNA-seq data analysis using GSEA, Gene Pattern platforms.
- ELISA, MSD-multiplex cytokine analysis, immunoblotting, IHC/IF techniques.
- CRISPR/Cas9 design and gene knockout and validation in mammalian cells.
- In vitro cell proliferation, cancer stemness, cytotoxicity assays and developing cell-based assays, qRTPCR, ddPCR.
- Mentored junior researchers and student trainees in lab.
- Team work to collaborate on 3 other ongoing projects in the lab.
Postdoctoral Associate
The George Washington University
Washington
01.2020 - 06.2022
- Independently led prostate cancer project to completion.
- Generation of variants of inducible knockout mouse models with 5 altered alleles.
- Worked on tamoxifen-inducible Cre/LoxP genetically engineered knockout mouse models, and spontaneous transgenic mouse model of prostate cancer.
- Developed and optimized culture conditions for 3D organoids from patient primary tumor, PDX-tumors and murine tumors.
- Generated xenografts from target gene knockout PDX-derived organoids.
- Developed and optimized organoid and xenograft freezing protocols.
- Mouse colony maintenance, breeding, genotyping, record keeping.
- Designed, optimized and performed multicolor flow cytometry assays for immunophenotyping of mouse prostate tumors.
- Collaborated on single cell genomics data analysis of tumors.
- Expertise gained in FFPE-tissue/organoid processing, sectioning and analysis by histochemistry, confocal fluorescence microscopy.
- Performed single cell genomics data analysis of tumors.
- Mentored 3 student trainees and projects.
Project Associate
Jawaharlal Nehru University
New Delhi
10.2018 - 12.2019
- Cell-based drug metabolism studies in normal and cancer cell systems.
Doctoral Research
Jawaharlal Nehru University
New Delhi
08.2013 - 02.2019
- Designed and optimized assays to track intracellular movement of drug metabolizing proteins.
- Expertise gained in cell-based drug metabolism assays in normal and cancer cell systems.
- Worked on in-house developed platforms for screening of drugs/xenobiotics capable of activating drug metabolism machinery.
- Work resulted in 6 research publications.
Quality Control Officer
Indoco Remedies Ltd.
Goa
05.2012 - 10.2012
- Analyzed pharmaceutical samples and intermediates by carrying out specific biochemical analysis.
Project Assistant
National Institute of Oceanography
Goa
11.2010 - 02.2012
- Analyzed water samples for pollution parameters like Biological Oxygen Demand, Chemical Oxygen Demand, Nitrates, pH.
Education
PhD - Molecular-Cell biology
Jawaharlal Nehru University
New Delhi, India02.2019
Master of Science - Biotechnology
Mangalore University
Mangalore, India07.2010
Bachelor of Science -
Goa University
Goa, India05.2008
Skills
- Project management
- Multicolor flow cytometry
- In vivo studies
- 3D organoid cultures
- Cell-based assay development
- CRISPR/Cas9 design
- RNA-seq analysis
- Q-RT-PCR techniques
- Confocal microscopy
- Immunohistochemistry (IHC)
- Immunofluorescence (IF)
- Western blotting
- ELISA assays
- In vitro proliferation assays
- Cancer stemness assessment
- Cytotoxicity testing
Major Publications
- Obesity, dysbiosis and inflammation: interactions that modulate the efficacy of immunotherapy, Ashutosh S Yende, Dipali Sharma, Front. Immunol., 15, 1444589, 10.3389/fimmu.2024.1444589
- Synbiotic supplementation improves immunotherapy response in obesity-associated breast cancer, Ashutosh S Yende, et al., Dipali Sharma, under preparation
- Targeting HDAC6 improves anti-CD47 immunotherapy, Gracia-Hernandez, M., Ashutosh S Yende., et al., J Exp Clin Cancer Res, 43, 60, 2024
- TRIM28 promotes luminal cell plasticity in a mouse model of prostate cancer, Ashutosh S Yende et al., Maho Shibata, Oncogene, 42, 17, 1347-1359, 2023
- Influence of signaling kinases on functional dynamics of nuclear receptor CAR, Ashutosh S Yende, Rakesh K Tyagi, Mol Cell Biochem, 461, 1-2, 127-139, 2019
- A comprehensive evaluation of anti-diabetic drugs on nuclear receptor PXR platform, Shashi Kala Singh, Ashutosh S Yende, Kalaiarasan Ponnusamy, Rakesh K Tyagi, Toxicol In Vitro, 60, 347-358, 2019
- Heterodimerization of Retinoid X Receptor with Xenobiotic Receptor partners occurs in the cytoplasmic compartment: mechanistic insights of events in living cells, Amit K Dash, Ashutosh S Yende, Bharti Jaiswal, Rakesh K Tyagi, Exp Cell Res, 360, 337-346, 2017
- Novel Application of Red Fluorescent Protein (DsRed-Express) for the Study of Functional Dynamics of Nuclear Receptors, Amit K Dash, Ashutosh S Yende, Rakesh K Tyagi, J Fluoresc, 27, 4, 1225-1231, 2017
Timeline
Scientist
Henry Jackson Foundation for the Advancement of Military Medicine
02.2025 - Current
Postdoctoral Fellow
Johns Hopkins Medicine
07.2022 - 01.2025
Postdoctoral Associate
The George Washington University
01.2020 - 06.2022
Project Associate
Jawaharlal Nehru University
10.2018 - 12.2019
Doctoral Research
Jawaharlal Nehru University
08.2013 - 02.2019
Quality Control Officer
Indoco Remedies Ltd.
05.2012 - 10.2012
Project Assistant
National Institute of Oceanography
11.2010 - 02.2012
PhD - Molecular-Cell biology
Jawaharlal Nehru University
Master of Science - Biotechnology
Mangalore University
Bachelor of Science -
Goa University
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