Azizur Rahman

Expert in immunology and cancer biology research with a solid foundation in B cell biology, T cell biology, immunotherapy, tumor microenvironment, lymph node tissue microenvironment, stromal cells, glioblastoma brain cancer, and host microbes' interaction. Possesses strong skills in manuscript and grant writing. Accomplished research professional with a Doctor of Philosophy (PhD) in Immunology and postdoctoral training in cancer biology.

• Immunotherapy Enhancement by Targeting Extracellular Tumor pH in Triple-Negative breast cancer mouse model, Md Azizur Rahman, Branislava Janic, Tasnim Rahman, Harshit Singh, Haytheme Ali, Ramandeep Rattan, Moshin Kazi and Meser Ali, Cancers, 10/23
• Transdermal entry of yeast components elicits transient B cell-associated responses in skin-draining lymph nodes, Md Azizur Rahman, Y Kanda, M Ozawa, T Kawamura, A Takeuchi, T Katakai, Cellular Immunology, 09/20
• Curcumin based nanoparticle induced U251 glioma cancer cell apoptosis via inhibition of HIF-1a and reverts cellular metabolic pathways towards mitochondrial respiration from activated glycolysis, Md Azizur Rahman, In preparation
• A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medul Boundary Subcompartment of the Lymph Node, Takeuchi A, Kanda Y, Kurosawa Y, Md. Azizur Rahman, Kawamura T, Shichida Y, Umemoto E, Miyasaka M, Takahama Y, Nagasawa T, Katakai T, Frontiers in Immunology, 10/18
• Essential Role of Canonical NF-KB Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs, Bogdanova D, Takeuchi A, Kanda Y, Md. Azizur Rahman, Ludewig B, Kinashi T, Katakai T, J Immunol, 12/18
• Curcumin reduces the risk of chronic kidney damage in mice with nonalcoholic Steatohepatitis by modulating endoplasmic reticulum stress and MAPK signaling, Afrin MR, Arumugam S, Rahman A, Karuppagounder V, Harima M, Suzuki H, Miyashita S, Suzuki K, Ueno K, Int. Immunopharmacol, 01/17
• Curcumin ameliorates liver damage and progression of NASH in NASH HC mouse model possibly by modulating HMGB1-NF-KB translocation, Afrin R, Arumugam S, Rahman A, Wahed MI, Karuppagounder V, Harima M, Suzuki H, Miyashita S, Suzuki K, Yoneyama H, Ueno K, Watanabe K, Int Immunopharmacol, 01/17
• Le Carbone, a charcoal supplement, modulates DSS-induced acute colitis in mice through activation of AMPKa and downregulation of STAT3 and caspase dependent apoptotic pathways, Afrin MR, Arumugam S, Rahman A, Karuppagounder V, Sreedhar R, Harima M, Suzuki H, Nakamura T, Miyashita S, Suzuki K, Ueno K, Int Immunopharmacol, 01/17
• Autophagy and Its Role in Modulating the Tumor Microenvironment in Breast Cancer: A Comprehensive Review, Azizur Rahman, In preparation for submission

• Flow cytometry: Multi-panel flow cytometry (including both intracellular and extracellular markers), CFSE analysis, Apoptosis analysis, cell cycles analysis, Cells sorting.
• Cell purification (MACS): T cells, B cells, Macrophages, stromal cell, Dendritic cells etc.
• Molecular biology: RNA and cDNA extraction, RT-qPCR, protein extraction, Western blot, MTT assay, ELISA.
• Cell Culture: In vitro cell culture systems such as different types of Immune cells, different types of cancer cell lines including glioblastoma. 3D cell culture, Organoid culture. Colony forming assay. In vitro cells migration assay.
• In Vivo Imaging System (IVIS): In vivo imaging of fluorescence and bioluminescence (BLI).
• Animal model: Mouse and rats' injection-drugs delivery and adaptive cell transfer. Murine tumor implant experience - Mammary fat pad, subcutaneous and stereotactic (Brain). Murine tissue (tumors, lymph nodes and other organs) harvesting, storage, and processing. Mouse and rats handling experience.
• Injection: Sub-Cutaneous (SQ), Intraperitoneal (IP), Tail vein injections (IV), Intertumoral (IT).
• Pathology: Tissue processing for histology both paraffin and frozen sections and various histochemical and immunohistochemical staining techniques.
• Computer skills: Microsoft Word, Excel, PowerPoint, Photoshop, Flowjo, Image J, Prism, Cytoflex, Ou-paths, Aperio versa.