Summary

Overview

Work History

Education

Skills

Accomplishments

Timeline

Benjamin Wronowski

Cincinnati,OH

Summary

Experienced laboratory researcher with a strong background in NGS sample preparation and analytical wet lab work. Skilled in routine testing, assay development, and proficient with associated equipment and software. Over 20 years of experience in the biopharmaceutical industry and academia, handling responsibilities from initial discovery experiments to small-scale production, purification, and USP testing on finished products. Collaborative mindset with extensive expertise in data collection, analysis, and adept problem-solving skills.

Overview

years of professional experience

Work History

Research Associate

University of Cincinnati

06.2022 - Current

Worked in the Leukemia & Drug Development Lab with a main focus on working with lab members to achieve their NGS needs.
Responsible for single-cell DNA sequencing library generation by means of the Tapestri workflow (MissionBio).
Worked with Dr. Anukanth on CAR-NK project
Processed human peripheral blood samples and bone marrow aspirates to isolate cells by Ficoll or antibody-conjugated magnetic bead separation
Molecular Biology techniques (DNA/RNA isolation, PCR, CRISPR-Cas9 KO)
Maintenance of cell cultures (cell lines and primary)
Ordered reagents
Maintained LIMS to keep track of sample information and location
Set up equipment, organized inventory and maintained shared lab spaces
Developed new protocols and improved existing laboratory processes.
Troubleshot and resolved equipment and process issues.
Trained and supervised new lab personnel and graduate students in laboratory protocols.
Validated incoming data to check information accuracy and integrity while independently locating and correcting concerns.
Participated in lab meetings and project presentations.

Research Assistant IV

CCHMC

09.2018 - 06.2022

Worked in the Dr. Artem Barski Laboratory focusing on epigenetics of memory T-cell activation.
Duties include performing ChIP-seq, ATAC-seq, RNA-seq library preparation on lab generated as well as collaborator generated samples.
Production and purification of pA/G MNase used to perform CUT&RUN assay/library generation for NGS.
Production and purification of pA/G-Tn5 and Tn5 enzymes for use in ChIP-seq, ATAC-seq, and CUT&TAG library generation for NGS.
Provide assistance to graduate students working in the Barski Lab.
Performed a majority of ordering for the lab.
I also shared responsibility for maintaining the Barski mouse colony housed at CCHMC.
Troubleshot and resolved equipment and process issues.
Assisted staff scientists by collecting and organizing laboratory data.
Provided support and guidance to graduate students
Coordinated and managed laboratory projects and experiments.
Developed new protocols and improved existing laboratory processes.

Research Assistant III

University of Oklahoma Health Sciences Center

04.2014 - 08.2018

Worked in the Dr. Willard Freeman Laboratory that was receiving NIH funding to study the epigenetics of aging, mitochondrial DNA heteroplasmy, and diabetic retinopathy.
Provide the bulk of wet lab research for the OUHSC Nathan Shock Center TDMMH Core.
Performed DNA/RNA extraction from cell culture and animal tissue.
Performed bisulfite treatment and cleanup of samples using Zymo Research kits.
Designed primers for Bisulfite Amplicon Sequencing experiments (BSAS).
QPCR and Western Blotting verification of TaqMan Array detected targets for various experiments.
Worked with Dr. David Stanford for long range mtDNA sequencing in single cells using Fluidigm C1 system.
Performed NGS sample prep for laboratory and collaborators and ran MiSeq system located in OUHSC sequencing core facility.
Worked with collaborators at Penn. St. Hershey Medical Center on HPV 16/18 head and neck cancer project by generating libraries utilizing Agilent SureSelect Targeted DNA Methylation kits.
Prepared samples for analysis by performing complex sample preparation tasks.
Assisted scientists by collecting and organizing laboratory data.
Analyzed results using analytical software and created reports.

Microbiology Lab Manager

ARL Biopharma

08.2011 - 01.2014

Managed a lab of 3 technicians that performed microbiological testing in accordance with USP guidelines on compounded pharmaceuticals.
Testing included USP Sterility Testing by both membrane filtration and direct inoculation, USP Bacterial Endotoxin Testing utilizing Charles River EndoSafe reagents and EndoScanV software, USP Total Microbial Counts Testing.
As manager I had to maintain a client formulation-specific sterility testing method validation database.
Created and revised department specific SOPs.
Initiated CAPAs and deviation reports for the department.
Responsible for final review of OOS reports.
Set and oversaw quality assurance guidelines for laboratory work.
Kept laboratory in compliance with applicable guidelines and laws.

Research Associate – Process Development Downstream Purification

Cytovance Biologics

11.2010 - 08.2011

Analyzed client technology transfer for scale-up/scale-down process.
Responsible for packing columns.
Performed CIP on packed columns.
Performed small-scale purification runs utilizing Akta Explorer FPLC with Unicorn software.
Performed routine maintenance and calibration on Akta Explorer.
Prepared buffers and associated SOPs for transfer to the manufacturing facility.
Worked with other department members on generating client progress reports.
Performed ultrafiltration/diafiltration using Millipore Pellicon system.

Sr. Research Investigator

Glycobiology Research Institute - Genzyme

04.2001 - 10.2010

Responsible for developing and running routine assays (4MU fluorescence enzyme activity assays, BCA and Bradford protein assays).
Generated N-glycan profile maps of company generated enzymes using endoglycosidase digestion and either RP-HPLC or HPAEC-PAD.
Characterized internally generated glycoproteins and antibodies using general and novel laboratory techniques.
Acid α-glucosidase (GAA) purification and characterization – Development of novel affinity purification technique that achieved greater than 90% purity with minimal loss for over 30 different GAA molecules generated through molecular engineering or glycan remodeling.
Novel Assay Development assisted in developing 96 well based assay for characterizing binding affinity of company generated antibodies against FGF23.
Helped in development of sCIM6PR 96-well assay to investigate ligand-receptor affinity of company generated enzyme replacement therapy preparations.
Developed internal expertise of solution based immunoassays (Perkin Elmer AlphaScreen technology).
Antibody Drug Conjugation: Investigated feasibility of using novel endo-glycosidase (EndoS), glycosyltransferase (B4GalT1)