Bianca Labuschagne
Denver,CO
Summary
Offering a strong foundation in biomedical research principles with a desire to learn and expand from the wet lab environment. Brings a thorough understanding of the biomedical research workflow and the perspective of clientele in cellular biology research, especially in cell therapy for cancer treatment. Excited and ready to apply my years of academic research experience to enhance customer experience as a Staff Scientist in Cell Therapy Field Applications for Thermo Fisher Scientific.
Overview
4
4
years of professional experience
Work History
Professional Research Assistant
Haverkos Lab, University of Colorado Anschutz
01.2024 - Current
- Utilized aseptic cell culture techniques for culturing cells including bacterial, mammalian, primary immune and immortalized cell lines.
- Prepared cell samples for flow cytometry analysis using SpectroFlow software on the CYTEK Aurora and analyzed resulting data using FlowJo software.
- Designed and generated genes of interest for cloning; purified, analyzed and confirmed DNA sequence of recombinant plasmid for subsequent use in bacterial transfection and lentiviral transduction of mammalian cells.
- Prepared samples for proteomic analysis and analyzed data received using QIAGEN Ingenuity Pathway Analysis software.
- Managed and maintained lab operations for a lab of 18+ graduate students and researchers, especially maintaining thousands of cell lines stored in liquid nitrogen for the lab, as well as general managerial tasks such as ordering, stocking reagents and supplies, scheduling and equipment maintenance.
- Collaborated within a team of multiple researchers and gained expertise in working across an interdisciplinary team while managing projects within a smaller team.
- Adhered to and aided in enforcing laboratory safety procedures to maintain compliance with quality control standards.
- Designed and executed experiments involving drug synergy screens, in-vivo and in-vitro experiments.
Undergraduate Researcher
Salogiannis Lab, University of Vermont
01.2022 - 05.2023
- Designed and executed experiments involving molecular cloning, recombinant DNA technologies, mammalian tissue culture, live-cell imaging, data analysis, and general lab maintenance.
- Analyzed data collected from experiments and presented findings at weekly lab meetings.
- Maintained laboratory equipment and reagents and ensured compliance with safety protocols, including BSL-I and BSL-II.
- Most recently involved in projects working on engineering molecular motor proteins for use in studying organelle hitchhiking.
Summer Research Intern
Salogiannis Lab, University of Vermont
06.2022 - 09.2022
- Prepared a detailed project proposal with help from my research mentor which ultimately led to receiving the UVM Summer Undergraduate Research Fellowships (SURF) Award to fund full-time summer research.
- Conducted independent research investigating the effects of inflammation on regulation of the Parkinson’s-linked LRRK2 gene expression at the transcriptional level.
- Planned and executed experiments involving molecular cloning technology, primer design, PCR and PCR variations, mammalian tissue cultures, gel electrophoresis, Western blotting, lentiviral transfection, bacterial transformation, and microscopy.
- Participated in and contributed to weekly lab meetings involving data presentations, departmental seminars, and journal clubs.
Undergraduate Teaching Assistant
Colorado State University
01.2021 - 06.2021
- Effectively communicated complex biology and physiology concepts to a class of 30+ undergraduate students to aid student comprehension of the material.
- Collaborated team to write and modify lesson plans to foster student growth in Laboratory in Principles of Physiology.
- Prepared and oversaw lab exercises while aiding student comprehension of the material.
- Ensured adherence to university, federal, state, and local Covid-19 policies.
Education
Bachelor of Science - Molecular Genetics
University of Vermont
Burlington, VT05-2023
Skills
Aseptic cell culture of bacterial, mammalian, primary immune and immortalized cell lines
Molecular biology techniques including design and generation of genes of interest for cloning, molecular cloning, and analysis and confirmation of DNA sequence of recombinant plasmid for proper protein expression
Flow cytometry and analysis of flow cytometry data using SpectraFlow and FlowJo software
Writing and preparation of grant proposals and manuscripts
Excellent communication and presentation skills
Effective and self-driven troubleshooting and problem-solving skills
Software: Microsoft Office Suite, Google Office, Benchling, SnapGene, BioRender, Graphpad Prism, QIAGEN Ingenuity Pathway Analysis, FlowJo, SpectraFlow
Self-motivated, highly adaptable and organized
Highly efficient in experimental planning, and logging and communicating plans and progress to supervisors
Timeline
Professional Research Assistant
Haverkos Lab, University of Colorado Anschutz
01.2024 - Current
Summer Research Intern
Salogiannis Lab, University of Vermont
06.2022 - 09.2022
Undergraduate Researcher
Salogiannis Lab, University of Vermont
01.2022 - 05.2023
Undergraduate Teaching Assistant
Colorado State University
01.2021 - 06.2021
Bachelor of Science - Molecular Genetics
University of Vermont
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