Christine Lee
Redmond,WA
Summary
Associate Scientist with 4+ years of experience in cell culture and a strong background in biological sciences. Specialized in potency assay development, T cell biology, and data analysis. Looking forward to contributing my skills and knowledge to the company.
Overview
2
2
years of professional experience
2
2
Language
Work History
Research Associate II
SystImmune
05.2023 - Current
- Oversaw project timelines to ensure on-time completion of deliverables with high-quality standards.
- Performed routine IncuCyte-based assays including Redirected T cell Cytotoxicity (RTCC), Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC), and proliferation assays.
- Conducted cell purification and immune cell phenotypic analysis using multi-color flow cytometry.
- Executed immunofluorescence techniques on cells and tissues. Analyzed data to derive meaningful insights and conclusions.
- Innovated by developing new protocols and enhancing existing laboratory procedures.
Research Assistant
University At Buffalo, SUNY
04.2018 - 04.2019
- Worked both independently and collaboratively in fast-paced laboratory environment.
- Performed statistical, qualitative, and quantitative analyses.
- Planned, modified, and executed research techniques, procedures, and tests.
- Gathered, arranged, and corrected research data to create representative graphs and charts highlighting results for presentations.
- Prepared materials for reports, presentations, and submissions to peer-reviewed journal publications.
RESEARCH ASSISTANT (Master's Program and PostBac)
University At Buffalo, SUNY
08.2015 - 04.2018
- Supported senior with the creation and enhancement of continuous improvement of research procedures.
- Supervised and mentored junior team members in laboratory techniques and operation of equipment.
- Responsible for stock checking and ordering reagents and materials.
- Responsible for cryopreservation of cell lines.
- Optimized primary cell isolation, culture, and cryopreservation from mice femurs and pig ribs to save the high cost of primary cells.
- Inputted and edited computer entries from generated research using GraphPad Prism, Image J, Zeiss ZenPro, SnapGene, and FCS Express.
Education
Master of Science - Pharmaceutical Sciences
University At Buffalo, SUNY
Buffalo, NY04.2018
Bachelor of Arts - Biochemistry
University of Washington
Seattle, WA06.2011
Skills
- Mammalian cell culture and transduction
- Isolation, purification, and culture of primary cell lines including human T, NK, and PBMC
- IncuCyte-based assays (RTCC, ADCC, T cell proliferation), multi-parameter flow cytometry for phenotypic characterization of T cells
- Multicolor flow cytometry
- Nucleic Acid delivery: miRNA, siRNA, mRNA transfection, and electroporation
- Immuofluorescence of cells and tissues
- RT-qPCR, PCR, Westernblot, ELISA, DNA cloning
- Conduct routine experimental tasks independently, following established protocols and procedures
- Organize and communicate findings promptly and effectively
- Software: Prism, FlowJo, FCS Express, Tableau, ImageJ, PowerPoint, Excel, Words
Timeline
Research Associate II
SystImmune
05.2023 - Current
Research Assistant
University At Buffalo, SUNY
04.2018 - 04.2019
RESEARCH ASSISTANT (Master's Program and PostBac)
University At Buffalo, SUNY
08.2015 - 04.2018
Master of Science - Pharmaceutical Sciences
University At Buffalo, SUNY
Bachelor of Arts - Biochemistry
University of Washington
Publications
- Scott Ferguson, Jinli Wang, Christine J. Lee, Maixian Liu, Sriram Neelamegham, John M Canty, Juliane Nguyen. The microRNA regulatory landscape of MSC-derived exosomes: a systems view. Scientific Reports Volume 8, 23 January 2018, Article number: 1419.
- J. Wang, Christine J. Lee, S. Ferguson, J. Nguyen. mir-101a loaded Extracellular Nanovesicles as Bioactive Carriers for Cardiac Repair. Nanomedicine: Nanotechnology, Biology, and Medicine, April 2020.
Similar Profiles
William SullivanWilliam Sullivan
Butcher at Fisher MeatsButcher at Fisher Meats
Dawit GebreslassieDawit Gebreslassie
Security Officer at Axum HotelSecurity Officer at Axum Hotel
Deepak JainDeepak Jain
Senior Manager –Supply Chain & Program Management at AmazonSenior Manager –Supply Chain & Program Management at Amazon
Colton HittColton Hitt
Baseball Coach at Velotech IncBaseball Coach at Velotech Inc
Nathaniel RobbinsNathaniel Robbins
Research Associate II at EpiCypherResearch Associate II at EpiCypher