William Lenh
Waltham,MA
Summary
Innovative researcher adept at understanding critical questions, designing efficient experiments, and generating meaningful results in the molecular/synthetic biology space. Experienced with identifying and deploying new technologies and resources. Works effectively and collaboratively with teams and individuals.
Overview
7
7
years of professional experience
Work History
Research Associate II
Stipple Bio
05.2024 - Current
- Validated hits and targets generated by the platform via flow cytometry
- Generated mammalian cell lines that stably express our targets for further validation studies
Research Associate
Resonance Medicine, Inc.
03.2022 - 12.2023
- Successfully conducted directed evolution campaigns using PANCE (phage-assisted non-continuous evolution) technology to reprogram a protease to cleave a therapeutic target.
- Designed, constructed, and implemented two separate arms of the PANCE evolution platform: one to increase enzymatic activity and another to eliminate off-target activity.
- Developed and established a PCR detection-based system for undesirable phage contaminants
- Conducted reporter assay screens to identify viable proteases/protease substrates
- Pioneered and implemented a directed evolution logic/decision tree system that resulted in 3-4 month acceleration of deliverables
- Cloned expression vectors, phage, and accessory plasmid libraries required for evolution campaigns
- Analyzed research data to create representative graphs and charts highlighting results for presentations for managers/other departments
Contract Research Associate
Fog Pharma
09.2021 - 03.2022
- Constructed focused and naive phage display libraries to discover peptides that have the ability to bind to key cancer targets such as KRAS, MDM2 and CHIP.
- Developed cloning methods for the modification of PVIII protein of M13 phage to investigate the phage viability and the effect of negative charges on resulting phage screens
Research Technician
NanoView Biosciences
03.2021 - 06.2021
- Maintained laboratory equipment, reagents, buffers, consumables, and certifications
- Planned and conducted experiments for assay development under the guidance of the senior scientist
- Hosted meetings within the company to share experimental data
Undergraduate Research Associate
Rosbash Lab
10.2017 - 05.2020
- Investigated the molecular and neural mechanisms behind temperature compensation of circadian rhythms on Drosophila melanogaster and the role of Doubletime (DBT) in temperature compensation
- Performed immunostaining, confocal microscopy, dissection of fly brains, DNA extraction/purification, and behavior experiments on Drosophila melanogaster
- Designed and constructed LUC reporter systems to analyze mRNA levels of specific gene products in specific neurons in Drosophila melanogaster
Education
Bachelor of Science - Biology
Brandeis University
Waltham, MA05.2020
Skills
- Flow Cytometry
- PCR
- Microscopy
- Immunohistochemistry
- RNA/DNA purification/extraction
- Assay development
- Data analysis
- DNA cloning (construct/primer design)
- Benchling/PRISM
- Aseptic Technique
- Library Construction
- Mammalian Cell Culture/Bacterial Cell Culture
Publications
- Schlichting M, Richhariya S, Herndon N, Ma D, Xin J, Lenh W, Abruzzi K, Rosbash M. Dopamine and GPCR-mediated modulation of DN1 clock neurons gates the circadian timing of sleep. Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2206066119. doi: 10.1073/pnas.2206066119. Epub 2022 Aug 15. PMID: 35969763; PMCID: PMC9407311.
Timeline
Research Associate II
Stipple Bio
05.2024 - Current
Research Associate
Resonance Medicine, Inc.
03.2022 - 12.2023
Contract Research Associate
Fog Pharma
09.2021 - 03.2022
Research Technician
NanoView Biosciences
03.2021 - 06.2021
Undergraduate Research Associate
Rosbash Lab
10.2017 - 05.2020
Bachelor of Science - Biology
Brandeis University
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