Designed and developed novel antibodies through molecular techniques such as PCR, cloning, and transfection, ensuring high specificity and sensitivity.
Conducted ELISA assays and established clonal cell lines for antibody production and validation, ensuring accurate expression and functionality.
Consultant
Blue Matter Consulting
South San Francisco, CA
06.2023 - 06.2024
Provided strategic consulting to biotech and pharmaceutical clients, delivering insights on market trends, competitive landscapes, and growth opportunities.
Collaborated with cross-functional teams to develop data-driven recommendations for product development, clinical trial strategies, and regulatory pathways.
Graduate Researcher
University of Utah
Salt Lake City, UT
08.2017 - 05.2023
Investigated the role of the Dux protein during embryonic genome activation using molecular biology techniques, tissue culture, and high-throughput sequencing to analyze protein interactions and chromatin changes.
Developed and optimized experimental protocols for studying early mammalian embryo development using mouse embryonic stem cells, contributing to a deeper understanding of gene regulation during embryogenesis.
Education
Ph.D. - Oncological Sciences
University of Utah
Salt Lake City, UT
05.2023
BS, Bachelor of Science in Biology, Minor in Chemistry -
Texas Christian University
Fort Worth, TX
05.2017
Skills
Mammalian Cell Culture
Confocal Microscopy
Western Blot / Immunoblots
PCR / RT-qPCR
Recombinant Protein Expression
ELISA
Coimmunoprecipitation
Molecular Cloning
Flow Cytometry
Bioinformatics
High-Throughput Sequencing
CRISPR Genome Editing
Publications
Smith Christina M., Grow Edward J., Shadle Sean C., Cairns Bradley R. (2023) Multiple repeat regions within mouse DUX recruit chromatin regulators to facilitate an embryonic gene expression program. eLife 12:RP87005.
Grow EJ, Smith CM, Weaver BD, Guo J, Stein P, Shadle SC, Hendrickson PG, Johnson NE, Butterfield RJ, Menafra R, Kloet SL, van der Maarel SM, Williams CJ, Cairns BR. (2021) p53 convergently activates Dux/DUX4 in embryonic stem cells and in facioscapulohumeral muscular dystrophy cell models. Nature Genetics. Aug;53(8):1207-1220.
Timeline
Scientist
MilliporeSigma / Experis
06.2024 - Current
Consultant
Blue Matter Consulting
06.2023 - 06.2024
Graduate Researcher
University of Utah
08.2017 - 05.2023
Ph.D. - Oncological Sciences
University of Utah
BS, Bachelor of Science in Biology, Minor in Chemistry -