Asif Iqbal, PhD
Baton Rouge,Louisiana
Summary
I am an experienced microbiologist and have been doing research in this field more than eight years. Working on different projects, I have gathered many skills, such as designing and writing research projects, implementing the projects, using different scientific instruments, helping the advisor to prepare NSF research grant, and writing research manuscript, etc. I can work independently or in a team and coordinate multiple projects at a time. The knowledge and skills I have gathered from my research and teaching will help me to be part of a prestigious research team, perform cutting-edge research, and solve critical microbiological problem.
Overview
9
9
years of professional experience
1
1
Certification
Work History
Postdoctoral Fellow
LSU School Of Veterinary Medicine
Baton Rouge, LA
02.2023 - Current
- Implemented the projects to decipher metabolic pathways involved in bicarbonate killing of human bacterial pathogens
- Actively led the projects and collaborated with other departments for resources sharing
- Acted as a group leader and provided mentorship for undergraduate and graduate students
- Routinely performed genetic manipulations in Gram-negative and Gram-positive bacteria
- Teamed with mass spectrometry facility scientists to measure metabolic precursors
- Performed proteomic analysis to identify novel bicarbonate metabolic pathway
- Investigated bacterial growth kinetics in aerobic and anaerobic conditions
- Presented research findings at national conferences
- Wrote research grants and manuscripts, ensuring high quality and timely submissions
Graduate Research Assistant
Biological Sciences, Louisiana State University
Baton Rouge, LA
08.2017 - 12.2022
- Completed the projects to discover the function of a membrane protein in Gram-negative bacterial pathogens.
- Developed a gene deletion method to delete gene in a non-model bacteria.
- Developed a methods to identify a bacterial toxin with TLC
- Discovered a flourometric assay to measure of membrane potential.
- Measured gene expression levels with RT-qPCR.
- Executed colorimetric assays to measure different precursors.
- Participated in weekly lab meetings for group discuss.
- Prepared and presented reports on research progress to faculty members.
- Trained undergraduate students, and drafted manuscripts for publication.
Research Assistant
Shahjalal University Of Science And Technology
Sylhet, Bangladesh
12.2014 - 04.2017
- Developed a biotechnology method for production of bacterial proteases
- Produced enzyme using bioreactor Electrolab FerMac 360
- Purified enzyme with ion-exchange chromatograph and SDS-PAGE
- Characterized kinetic properties of enzyme
- Prepared reports, presentations and articles using Microsoft office
Education
Ph.D. - Biochemistry
Louisiana State University And A&M College
Baton Rouge, LA12-2022
Master of Science - Genetic Engineering And Biotechnology
Shahjalal University of Science And Technology
Sylhet, Bangladesh09-2014
Bachelor of Science - Genetic Engineering And Biotechnology
Shahjalal University of Science And Technology
Sylhet, Bangladesh 09-2012
Skills
Molecular Biology and Microbiology
Antibiotics sensitivity assays
Bacteria growth curve in anaerobic chamber
DNA isolation and sequencing
Agarose gel electrophoresis
Primer design, PCR, gene cloning
Gibson assembly
Gene knockout with homologous recombination
Genomic library construction and screening
Generation of conditional mutant
RNA isolation and RT-qPCR
Proteomic analysis of samples
Thin layer chromatography (TLC)
Fermentation and Protein Purification
Electrolab FerMac 360
DEAE-cellulose column chromatography
SDS-PAGE
Microscopy and Spectrometer
DM6 fluorescence microscopy
JASCOFP-6300 spectrofluorometer
Bioinformatics
NCBI, PubMed, GenBank, BLAST, KEGG,
UniPortKB, Clustal Omega, and TMHMM 20l
Computer skills
Microsoft office, ImageJ, EndNote, SnapGene, BioRender, GraphPad Prism, and JMP
Languages
English
Full Professional
Bengali
Native/ Bilingual
Affiliations
Served election commissioner for the BSA Election 2023
Former member of Biograd society, LSU Biological Sciences
Member of American Society of Microbiology (ASM) since 2021
Member of Bangladeshi Students Association (BSA) at LSU since August 2017
Accomplishments
2022 Ron and Mary Neal Distinguished Graduate Student Fellowship, LSU
Awarded one semester fellowship ($11,220) and cover fees and tuition
2022 Simon Chang Biochemistry Support Fund, LSU
Awarded $2000 to enhance learning experience of outstanding student pursing research in biochemistry
2019 LSU BioGrads Award ($400), Biological Sciences, LSU
2017 – 2022 LSU offered graduate research and teaching assistantship
Certification
- Certificate for attaending 3rd internation conference on Staphylococcal infections
Selected Publications
- Iqbal, et al. (2023). A Membrane protein of the rice pathogen Burkholderia glumae required for oxalic acid secretion and quorum sensing. Wiley, MPP.
- Iqbal, et al. (2023). Transcription factor PecS mediates agrobacterium fabrum fitness and survival. ASM, JBac.
- Iqbal et al. (2021). Chemical or genetic alteration of proton motive force results in loss of virulence of Burkholderia glumae, the cause of rice bacterial panicle blight. ASM, AEM.
Research articles highlighted in Journal blog
Asif Iqbal. 2023. Newly discovered protein helps a rice pathogen avoid alkaline toxicity. Wiley, MPP Highlight Blog.
Asif Iqbal. 2023. Alteration of Proton Motive Force Results in Loss of Burkholderia glumae Virulence. American Society of Microbiology, AEM Spotlight.
References
References available upon request.
Timeline
Postdoctoral Fellow
LSU School Of Veterinary Medicine
02.2023 - Current
Graduate Research Assistant
Biological Sciences, Louisiana State University
08.2017 - 12.2022
Research Assistant
Shahjalal University Of Science And Technology
12.2014 - 04.2017
Ph.D. - Biochemistry
Louisiana State University And A&M College
Master of Science - Genetic Engineering And Biotechnology
Shahjalal University of Science And Technology
Bachelor of Science - Genetic Engineering And Biotechnology
Shahjalal University of Science And Technology
- Certificate for attaending 3rd internation conference on Staphylococcal infections
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