Daisy Matsa-Dunn
Seattle,Washington
Summary
Accomplished and passionate Immune monitoring specialist with over 20 years experience in molecular biology, quality control and cell-based potency assays. Demonstrates expertise in procedure development and productivity improvement, driving innovation with a strategic vision.
Overview
20
20
years of professional experience
Work History
Immune Monitoring Specialist II
Fred Hutch Cancer Research Center
04.2018 - Current
- Working directly with the Immune Monitoring unit head to design, develop, and execute bench experiments; in charge of planning, and coordinating to conduct the development of translational clinical immunotherapy for cancer utilizing a variety of cutting-edge translational CAR-T cells and molecular techniques in patient samples with hematologic malignancies.
- Independent judgement is used in interpreting and applying study protocol, overseeing, managing, and assisting staff.
- Enforce adherence to SOPs including Good Lab Practices and HIPPA regulations.
- Performed FLAP, VSVG, WPRE, hCD19, ALB quantitative PCR, ddPCR, and dPCR to monitor and QC CAR-T leukemia and lymphoma clinical trials.
- Generated data for immunosequencing to profile many T-cell and B-cell receptors in a one sample by ImmunoSEQ assay and TaKaRa SMARTer human and mouse TCR a/b profiling that expand the understanding of the adaptive immune system
- Assembled DNA constructs using Gibson Assembly by SGI-DNA, a tool that used to rapidly clone multiple DNA fragments into a vector to create human leukocyte antigen for tetramers used by FRCRC researchers as well as others
- Performed TREC assay PCR for bone marrow transplantation clinical trial testing a treatment that is a landmark breakthrough for leukemia patients done by Delaney lab
- Prepared protein, monomers, and tetramers to be used as detection tools of antigen specific T cells needed by our clients
- Performed persistence tracking by flow cytometry analysis for WT-1 tetramer specific CAR T cells for Greenberg lab clinical trials
- Performed Cytokines assay and analysis (over 50 Cytokines panel) using Luminex microbead methodology and ELISA assays for verity of clinical trials.
Research Scientist II
Seattle Children’s/ University of Washington- Allergy & Infectious Diseases
03.2015 - 08.2017
- Executed administrative tasks for the Chu laboratory including financial reconciliation, negotiated with biotech vendors, scheduled training sessions, and ordered laboratory and office supplies
- Led the start-up of a laboratory in a UW Medicine (Harborview medical Center Institute, Research & Training Build)
- Analyzed and evaluated the transmission of maternally derived RSV antibody by Micro Neutralization Assay, Total IgG, and Total IgA ELISA tests for clinical trial samples (Nepal, Alaska, and WA State)
- Maintained mammalian cell lines, and generated cell banks
- Created and managed freezer database
- Quantified human cytokines in maternal and blood cord serum in Magnetic 25-Plex using Luminex 200 analyzer with xPONENT software
- Validated and QC instruments in the lab (Luminex 200, BioTech 405TS Plate Washer, BioTech Plate reader) Evaluated whether maternal vaccination for RSV has the potential to protect infants from RSV disease
- Created and maintained clinical trial samples database
- Troubleshoot and wrote SOP for Micro Neutralization Assay
- Executed and maintained the growth and stock of Respiratory Syncytial Virus (RSV)
- Recruited and evaluated student applicants for the Chu laboratory research team
- Updated laboratory systems for digital file conversion, software updates, and Internet installation
- Responsible of tissue culture room
- Responsible of teaching and guiding two students work employees at the lab
- Created and implemented Standard Operating Procedures (SOPs) for the laboratory
- Presented experiment results and findings in weekly laboratory meeting.
Research Scientist I
University of Washington- Periodontics Department
03.2004 - 02.2012
- Project leader investigating regulators of mineralization in a specialized cellular model system
- Recruited and evaluated student applicants for the Somerman laboratory research team
- Acted as a liaison across multiple departments (Comparative Medicine and Oral Biology)
- Supervised four undergraduate projects
- Executed administrative tasks for the Somerman laboratory including financial reconciliation, negotiated with biotech vendors, scheduled training sessions, and ordered laboratory and office supplies
- Updated laboratory systems for digital file conversion, software updates, and Internet installation
- Coordinated paperwork and schedules to ease the transition for visiting professors and students
- Investigated the interactions between Vitamin D and ionic phosphate on expression and mineralization factors by transient and stable transfection
- Researched and investigated murine cementoblast cell culture along with quantitative gene expression
- Developed and implemented a non-radioactive In Situ hybridization protocol
- Executed PCR based testing procedures for in-house mammalian cells
- Responsible for all In Situ hybridization experiments in the lab
- Advised and taught fellow researchers, both graduate and undergraduate, students in laboratory procedures such as cell culture, quantitative gene expression, Real Time PCR, Sequencing, Plasmid generation, Immunohistochemistry, and enzymatic assays
- Analyzed and troubleshoot Immunohistochemistry including microscopic evaluations, and documentation of results and images
- Supported the lab histology core, embedding mouse’s tissues in paraffin
- Maintained mammalian and stem cells in tissue culture room
- Investigated signaling pathway analysis and promoter analysis of dental matrix protein (DMP-1), a crucial regulator of mineralization of bone and dentin
- Analyzed and isolated large quantities of high-quality plasmid DNA for use in dental stem cells transfection
- Extracted DNA and RNA from blood, tissue, and bone
- Performed animal studies including transgenic mouse handling, euthanizing, and collection of blood, tissue harvesting and genotyping
- Presented Experiment results and findings in weekly laboratory meeting
- Managed lab’s supply and reagents
Education
Medical Technology Science program -
University of Washington
Seattle, WA01.2014
Master's degree - Human Molecular Genetic
Tel Aviv University
Tel Aviv Israel01.2003
Master of Arts - Medical Technology Science
Hadassah College of Technology, Israel
Jerusalem, Israel01.2002
Associate of Science - Medical Technology
Hadassah College of Technology
Jerusalem, Israel06.1991
Skills
- Data Analysis
- Procedure Development
- Project Management
- Compliance Monitoring
- Technical Support
- Student Management
- DNA/RNA Extraction
- Genotyping
- Cytokines Assays
- ELISA Assays
- Plasmid Generation
- In Situ Hybridization
- TCR Sequencing
- Real Time PCR / DD PCR/ dPCR
- Neutralization Assay
- Immunohistochemistry
- Tissue Culture
- Flowcytometry
Goals
Be part of a team that will change the way cancer and other serious diseases are treated and diagnosed.
Languages
English
Native or Bilingual
Hebrew
Native or Bilingual
Greek
Limited Working
Timeline
Immune Monitoring Specialist II
Fred Hutch Cancer Research Center
04.2018 - Current
Research Scientist II
Seattle Children’s/ University of Washington- Allergy & Infectious Diseases
03.2015 - 08.2017
Research Scientist I
University of Washington- Periodontics Department
03.2004 - 02.2012
Medical Technology Science program -
University of Washington
Master's degree - Human Molecular Genetic
Tel Aviv University
Master of Arts - Medical Technology Science
Hadassah College of Technology, Israel
Associate of Science - Medical Technology
Hadassah College of Technology
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