Summary
Overview
Work History
Education
Skills
Timeline
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Danielle Phebus

Summary

Adaptable Scientist with a background in mRNA production and assay development. Possesses strong skills in experimental design, data interpretation, and technical report writing. Known for organization and a collaborative work style. Demonstrated ability to contribute to impactful research projects and collaborate effectively with interdisciplinary teams.

Overview

5
5
years of professional experience

Work History

Associate Scientist II

Prime Medicine
Cambridge, MA
10.2022 - Current
  • Assisted in the creation of robust plasmid linearization, in vitro transcription, and mRNA purification assays to sustain the demands of mRNA production for the organization's liver, lung, hematology, and immunology pipelines.
  • Maintained weekly mRNA production via in vitro transcription to support research and development platforms within the organization.
  • Evaluated the purity of processed mRNA samples using various standard techniques, such as the UnChained Labs LUNATIC UV/Vis spectrophotometer and the Agilent Fragment Analyzer.
  • Experienced in using various mRNA production and purification methods, such as QIAGEN RNeasy Maxi Spin Columns, UnChained Labs' Big TUNA high-throughput buffer exchange platform, and AKTA chromatography.
  • Analyzed data, interpreted results, and documented findings accurately in the laboratory electronic notebook, Benchling.
  • Experienced in cell-free assay development using the Promega Rabbit Reticulocyte Lysate kit.
  • Trained team members and interns on plasmid digestion, linearization and purification, mRNA production and purification assays.

Associate Scientist I

Elevate Bio
Waltham, MA
03.2022 - 10.2022
  • Processed and purified circular RNA to support the demands of the organization's research and development programs.
  • Examined circular RNA purity via gel electrophoresis and ATKA chromatography.
  • Collaborated closely with peers and supervisors throughout the research process to analyze and interpret circular RNA data.

Medical Technologist I

Quest Diagnostics
Marlborough, MA
08.2020 - 03.2022
  • Utilized real-time PCR to detect several types of bacteria responsible for tick-borne diseases, such as Lyme disease, within whole blood, synovial fluid, and cerebrospinal fluid patient samples.
  • Proficient in operating the MagNa Pure 96 DNA extraction and purification instrument, the Cepheid GeneXpert System, the Hamilton STARlet aliquoting instrument, and the Thermo Fisher 7500 Fast ABI instrument for real-time PCR.
  • Participated in procedure and instrument development activities, such as performing instrument-to-instrument comparability and validations.
  • Identified the patient and labeled specimens with the correct information to avoid adverse errors.
  • Responsible for training other medical technologists to perform and interpret tests related to the tick-borne disease diagnostics assays during their job rotation within the molecular biology department.

Education

Master of Science - Biotechnology

Worcester State University
Worcester, MA
05-2020

Bachelor of Science - Biotechnology

Worcester State University
Worcester, MA
05-2019

Skills

  • mRNA production
  • mRNA purity analysis
  • Plasmid linearization
  • mRNA production assay development
  • Plasmid digestion, purification, assay development
  • Teamwork and collaboration
  • Gel electrophoresis
  • In vitro transcription
  • mRNA purification
  • PCR techniques

Timeline

Associate Scientist II

Prime Medicine
10.2022 - Current

Associate Scientist I

Elevate Bio
03.2022 - 10.2022

Medical Technologist I

Quest Diagnostics
08.2020 - 03.2022

Master of Science - Biotechnology

Worcester State University

Bachelor of Science - Biotechnology

Worcester State University

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Danielle Phebus