Daun Everforest
Senior Technical Application Scientist
San Marcos,CA
Summary
Determined and well-rounded Technical Scientist with more than 15 years experience within Biotech industry as well as academia. Sharp and focused professional offering skills in synthetic biology, molecular DNA and recombinant biology, NGS, immunoassay development, RNAi, CRISPR, ELISA, FACS, fluorescent in-vivo imaging, PK-PD, Immunogenicity, micro-fluidics and next-gen sequencing. Proven ability in development of novel applications and experimental design. Expertise in Salesforce, Excel, Powerpoint, SAS
Overview
48
48
years of professional experience
9
9
years of post-secondary education
Work History
Senior Technical Applications Scientist
CodexDNA
San Diego, CA
01.2022 - Current
- Develop and execute laboratory workplans/schedules for self and team, using project development milestones and division performance.
- Interact with internal staff and management to identify assays requiring further improvements or development and oversees improvement of these assays.
- Supervise technical staff, including work assignment, performance and development management.
- Assist in development of strategies for process improvements.
- Assist in training junior level staff with goal of guiding/mentoring all members of development team.
- Interpret, evaluate, communicate and properly document results of development work and make recommendations for action.
- Helped transform customer experience by implementing new support tools and processes.
- Developed, wrote and edited technical documentation and training materials for internal use.
- Developed conversion and system implementation plans.
- Sought and developed process improvements to provide service and support, meet revenue targets and improve customer satisfaction.
Lead Scientist
Proper Cell Bio
San Diego, CA
02.2019 - 01.2022
- Simultaneously managed several complex projects, meeting all pertinent milestones.
- Trained new employees on areas of technical expertise and compliance issues relevant to lab setting.
- Applied theories and methods of life sciences to interpret and perform analyses of experimental results.
- Conducted document review, organization and quality control of data acquisition to draw relevant conclusions and direct research activities.
- Advised or assisted in obtaining patents or meeting other legal requirements.
Faculty Instructor
California State University
San Marcos
08.2013 - 01.2022
- Molecular Cell Biology Lecture and Lab, Microbiology Lecture and Lab, Biotechnology Lecture and Lab, Biotechnology Professional Masters Program.
- Design and development of molecular biology laboratory courses for graduate and undergraduate University students.
- Develop current protocols and presentations designed for mastery of molecular biology lab.
- Lab curriculum and assay preparation including CRISPR-Cas modification in S.
- Pombe, Recombinant protein expression and purification, ELISA, Western blot analysis, FACS, DNA cloning, qPCR, Gibson cloning, RNA/DNA extraction,.
- Phylogeography of Critically Imperiled Grand Canyon Masked Clubskimmer (Brechmorhoga pertinax).
Research Scientist Molecular Genetics
San Diego Zoo Institute for Conservation Research
03.2013 - 08.2015
- DNA bar-coding.
- Genomics.
- Biostatistics.
- Genetic analysis.
- Population and genome analysis studies.
Field Application Scientist
LI-COR Biosciences
08.2009 - 10.2012
- Technical Applications Scientist with extensive assay development skills including immunoassay, RNAi, ELISA, in vivo fluorescent imaging.
- Prepare and deliver technical presentations to scientific audiences.
- Help maintain and increase product usage, and support sales efforts through technical presentations and demonstrations.
- Provide pre-sale and post-sale support including applications, experimental design, reagent support.
- Provide front-line troubleshooting of issues related to user's technical skills, biology/ assay design and experimental design.
- Develop and maintain positive relationships with customers in assigned territory.
- Work with Sales Consultants/Product Managers on commercial initiatives aimed at defining customers’ needs for new and improved products and services.
- Work with development teams to identify and help characterize new applications in Western detection and in-vivo imaging and relate these applications to capabilities and products to allow the capture of new markets.
- Help specify and develop technical support materials and user manuals for new and current products.
- Work with the Marketing team to contribute to journal articles, applications notes and other marketing and technical collateral to support products at seminars, scientific meetings and trade shows.
- Work with other team members to develop sales and field applications technical training programs and participate in their execution.
- Act as a key contributor of customer feedback to Marketing and Product Development teams.
- Develop and improve key company processes that support building a world-class customer support organization.
Adjunct Faculty
California State University
San Marcos
09.2006 - 01.2010
- Courses taught:.
- Molecular Cell Biology, lecture and lab.
- RNA and Genomics Technologies for Masters.
- Conservation Biology.
- Human Nutrition.
- Human Reproduction.
- Cancer Biology.
- Masters Molecular Biology Seminar.
- Course design for Biostatistics for Nurses.
Field Application Scientist
Gyros, Inc
09.2007 - 08.2009
- West Coast Representative for Applications for novel detection of immunoassays, ADA, ATA.
- Assay development including biomarker identification, PK/PD assay, Immunogenicity.
- Immunoassay development for new and existing customer accounts.
- In-depth customer training and individualized assay design.
- Coordinate and lead presentations for potential West coast customers.
- Organized conference poster presentations/demonstrations.
- Assist in technical/software applications and data analysis.
- CRM management.
Post-doctoral Researcher
California State University
San Marcos
05.2006 - 09.2007
- Laboratory supervisor for team of graduate and undergraduate students.
- Validation of high-density oligonucleotide microarray analysis using QPCR.
- Coordinator and member of Annotation Team for genome annotation the model coccolithophorid Emiliania Huxleyi, focusing on genes involved in dimethylsulfide emissions in E.
- Huxleyi,.
- Development of genetic transformation system for marine coccolitophorids using biolistics and cationic lipid based technologies.
- Recombinant vector design for expression and detection of in-vitro transcribed RNAs in coccolithophorids.
- Novel protein expression and purification in S.
- Cerevisea.
Graduate Research Scientist
San Diego State University Research
San Diego, CA
07.2002 - 06.2021
- Dr Skaidrite Krisans Dissertation Project: Identification and Characterization of Novel Isopentenyl Diphosphate Isomerase in Humans.
- Expression and purification of novel protein in bacteria and yeast systems.
- Development of in-vitro cell based and enzymatic assays.
- Testing and identifying enzyme kinetics in vitro.
- Complementation testing in vivo in S. cerevisiae.
- Protein expression in cultured cells.
- Immunofluorescence and analysis of expressed proteins by confocal microscopy.
- Prepared annual NIH Grant updates, prepared and submitted AHA Grants.
- Wrote research papers, reports, reviews and summaries regarding isoprenoid biosynthesis.
Graduate Research Assistant
San Diego State University
09.1997 - 01.2001
- Project: Investigation of the Transcription/Replication Regulatory Mechanisms in the Vesicular Stomatitis Virus (VSV) L protein.
- Site-directed mutagenesis of VSV L protein created mutation within a region identified as playing a role in regulation of replication and transcription in VSV.
- Molecular cloning and recombinant VSV L protein expression and purification.
- Transcriptional enzyme assay design, development and implementation.
Research Assistant
California State University
San Marcos
08.1996 - 06.1997
- Use of Microsatellite Markers in the Hybridization Rate of two closely related species of Bluegill Sunfish (Lepomis Macrochirus).
- Genetic diversity analysis of captive populations of penguins using molecular genetic techniques.
Research Fellow
Center For Reproduction, Endangered Species, Molecular Genetics
05.1995 - 06.1996
- Use of Enrichment Technique for Creation of Microsatellite Library from Genomic Black Rhino DNA.
- Project involved use of non-invasive techniques for isolation of genomic DNA from endangered population of captive Black Rhino.
- Construction of genomic library enriched for microsatellite sequences.
- Evaluation of microsatellite sequences as individual specific loci for the purpose of unambiguous identification of individuals as well as degrees of relatedness within a captive population of endangered black rhino.
Education
Bachelor of Science - Biological Science
California State University
Ph.D. - Cell and Molecular Biology
University of California San Diego, San Diego State University
06.1997 - 05.2006
Skills
Assay development
Quantitative analysis
Training and mentoring
Research implementation
Microsoft Excel proficiency
Leadership
Collaboration
Self-Motivated
Research and experiments
Experiment Design
Excellent time management abilities
Presentation management
Method validation
Accomplishments
- Supervised team of 8-10 staff members.
- Achieved platform launch by completing testing and implementation with accuracy and efficiency.
- Documented and resolved multiple product issues which led to customer project design resolution.
Affiliations
American Society of Virology (ASV)
American Association for Advancement of Science (AAAS)
Additional Information
- HONORS AND AWARDS , ARCS Foundation Scholar 1999-2005 Cum Laude, CSUSM 1997 NSF-CRES, San Diego Zoo Fellowship 1995-1996 Kellogg Undergraduate Scholarship 1993-1995
Awards
ARCs Scholar UCSD 1997-2006
Outstanding Lecturer Award, College of Biological Science , California State, University San Marcos 2019
President's Award for Outstanding Lecturer, California State, University San Marcos 2021-22
Timeline
Senior Technical Applications Scientist
CodexDNA
01.2022 - Current
Lead Scientist
Proper Cell Bio
02.2019 - 01.2022
Faculty Instructor
California State University
08.2013 - 01.2022
Research Scientist Molecular Genetics
San Diego Zoo Institute for Conservation Research
03.2013 - 08.2015
Field Application Scientist
LI-COR Biosciences
08.2009 - 10.2012
Field Application Scientist
Gyros, Inc
09.2007 - 08.2009
Adjunct Faculty
California State University
09.2006 - 01.2010
Post-doctoral Researcher
California State University
05.2006 - 09.2007
Graduate Research Scientist
San Diego State University Research
07.2002 - 06.2021
Graduate Research Assistant
San Diego State University
09.1997 - 01.2001
Ph.D. - Cell and Molecular Biology
University of California San Diego, San Diego State University
06.1997 - 05.2006
Research Assistant
California State University
08.1996 - 06.1997
Research Fellow
Center For Reproduction, Endangered Species, Molecular Genetics
05.1995 - 06.1996
Bachelor of Science - Biological Science
California State University