Summary
Overview
Work History
Education
Skills
Publications
Abstracts
Laboratory Skills
Accomplishments
Timeline
Generic

David Hawkins

Philadelphia,Pennsylvania

Summary

Dynamic scientist with extensive experience in forensic and research science, specializing in nucleic acid purification and analysis. Issued over 800 DNA reports and provided expert testimony in 60 court cases. Recognized for strong analytical skills and a collaborative approach, ensuring quality and accuracy in high-pressure environments.

Overview

34
34
years of professional experience

Work History

Forensic Scientist 3

OFFICE OF FORENSIC SCIENCE - PHILA. POLICE DEPT.
Philadelphia, USA
04.2008 - 03.2025
  • Analyzed evidence and reference materials, and issued more than 800 forensic DNA reports to provide investigative leads in various criminal investigations.
  • Created the workflow, managed the PPD DNA Lab's case outsourcing program, and performed more than 1,000 technical reviews of outsourced case files.
  • Performed more than 600 technical reviews of my peers' case files to ensure adherence to quality control and quality assurance requirements.
  • I testified as an expert in the field of forensic DNA analysis in approximately 60 criminal trials in the Commonwealth of Pennsylvania, and three trials in the Federal Court of the Eastern District of Pennsylvania.
  • Performed scheduled maintenance and performance checks for various instruments within the DNA Laboratory.

Research Technician

THOMAS JEFFERSON UNIVERSITY
Philadelphia, USA
07.1991 - 07.2007
  • Worked independently on multiple projects employing complex molecular biology techniques.
  • Assisted in grant writing and manuscript preparation.
  • Maintained laboratory records according to Good Laboratory Practices.
  • Assisted in the training of newly hired technicians and instructed/demonstrated molecular biology techniques to undergraduate and graduate students during lab rotations.
  • Entrusted with daily lab management and maintenance duties.

Education

Associate of Science - Medical Laboratory Technology

HAHNEMANN UNIVERSITY
PHILADELPHIA, PENNSYLVANIA

Bachelor of Arts - Biology

TEMPLE UNIVERSITY
PHILADELPHIA, PENNSYLVANIA

Skills

  • Nucleic acid purification (manual and automated)
  • Real-time qPCR
  • PCR (multiplex and conventional)
  • Capillary electrophoresis
  • Blotting techniques (Northern, Southern, Western)
  • SDS-PAGE
  • ChIP assay
  • DNase I footprinting
  • Plasmid construction
  • Immunofluorescence and microscopy
  • Radioisotope handling
  • Tissue culture
  • Cell transfection
  • Adenovirus production
  • Sequence analysis software
  • Laboratory management and maintenance
  • Microsoft Office Suite (Word, Excel, PowerPoint)
  • Genemapper IDX software
  • CODIS database management
  • Vector NTI software
  • DNA profiling
  • Technical review
  • Quality control
  • Data interpretation
  • Regulatory compliance

Publications

  • S. Piera-Velazquez, D.F. Hawkins, M.K. Whitecavage, D.C. Colter, D.G. Stokes, S.A. Jimenez, "Regulation of the human Sox9 promoter by Spl and CREB", Experimental Cell Research, 313, 2007, 1069-1079
  • D.C. Colter, S. Piera-Velazquez, D.F. Hawkins, M.K. Whitecavage, S.A. Jimenez, D.G. Stokes, "Regulation of the human Sox9 promoter by the CCAAT-binding factor", Matrix Biology, 24, 2005, 185-197
  • I.B. Coimbra, S.A. Jimenez, D.F. Hawkins, S. Piera-Valazquez, D.G. Stokes, "Hypoxia inducible factor-I alpha expression in human normal and osteoarthritic chondrocytes", Osteoarthritis and Cartilage, 12, 4, 2003, 336-345
  • D. Stokes, G. Liu, R. Dharmavaram, D. Hawkins, S. Piera-Velazquez, S.A. Jimenez, "Regulation of type-Il collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific phenotype in culture involves Sry-type high-mobility-group box (SOX) transcription factors", Biochemical Journal, 360, 2001, 461-470
  • G.R. Dodge, D.F. Hawkins, E. Boesler, L. Sakai, S.A. Jimenez, "Production of Cartilage Oligomeric Protein (COMP) by Cultured Human Dermal and Synovial Fibroblasts", Osteoarthritis and Cartilage, 6, 6, 1998, 435-440
  • P.J. Christner, L.D. Siracusa, D.F. Hawkins, R. McGrath, J.K. Betz, S.T. Ball, S.A. Jimenez, J. Peters, "A High-Resolution Linkage Map of the Tight Skin 2 (Tsk2) Locus: a Mouse Model for Scleroderma and Other Cutaneous Fibrotic Diseases", Mammalian Genome, 7, 8, 1996, 610-612
  • P.J. Christner, J. Peters, D.F. Hawkins, L.D. Siracusa, S.A. Jimenez, "The Tight Skin 2 Mouse - An Animal Model of Scleroderma Displaying Cutaneous Fibrosis and Mononuclear Cell Infiltration", Arthritis Rheumatism, 38, 12, 1995, 1791-1798

Abstracts

  • G.R. Dodge, D.F. Hawkins, S.A. Jimenez, "Modulation of aggrecan, MMPI, and MMP3 production by glucosamine sulfate in cultured human osteoarthritis articular chondrocytes", Arthritis Rheumatism, 42, suppl., 1999, S253
  • S. Grässel, M. L. Chu, R. Timple, D. Hawkins, G.R. Dodge, "Type XVI collagen is expressed in human articular chondrocytes and cultured synovial cells", American College of Rheumatology 64th Annual Scientific Meeting, San Diego, November, 1998
  • P.J. Christner, R. McGrath, D.F. Hawkins, L.D. Siracusa, S.A. Jimenez, "A mutation in the fibrillin gene influences al (I) procollagen mRNA levels in Tsk mouse embryonic cell lines", American College of Rheumatology 60th Annual Scientific Meeting, Orlando, October, 1996
  • P.J. Christner, L.D. Siracusa, D.F. Hawkins, R. McGrath, J.K. Betz, S.T. Ball, S.A. Jimenez, J. Peters, "A high-resolution linkage map of the tight skin 2 (Tsk2) locus: a mouse model for scleroderma (SSc) and other cutaneous fibrotic diseases", American College of Rheumatology, 60th Annual Scientific Meeting, Orlando, October, 1996
  • P.J. Christner, J. Peters, D.F. Hawkins, L.D. Siracusa, S.A. Jimenez, "Preliminary characterization of a novel tight skin mutation in the mouse: Tsk2", 14th Annual East Coast Connective Tissue Meeting, Baltimore, March, 1994
  • P.J. Christner, L.D. Siracusa, J. Peters, D.F. Hawkins, S.A. Jimenez, "Tsk2, A novel animal model of Scleroderma: Biochemical, Histopathological, and Genetic Characterization", American College of Rheumatology 58th Annual Scientific Meeting, Minneapolis, October, 1994

Laboratory Skills

  • Purification of nucleic acids
  • Real-time PCR
  • Multi-plex & Conventional PCR
  • Capillary electrophoresis
  • Northern blot analysis
  • Southern blot analysis
  • SDS-PAGE
  • Western blot analysis
  • ChIP assay
  • DNAse I footprinting
  • Plasmid construction
  • Immunofluorescent and fluorescent staining and microscopy
  • Radioisotope handling
  • Extensive tissue culture experience
  • Cell transfection and adenovirus production
  • Computer sequence analysis
  • General laboratory maintenance and management

Accomplishments

  • Entrusted to work on many cold case and high profile criminal investigations that lead to criminal convictions.

Timeline

Forensic Scientist 3

OFFICE OF FORENSIC SCIENCE - PHILA. POLICE DEPT.
04.2008 - 03.2025

Research Technician

THOMAS JEFFERSON UNIVERSITY
07.1991 - 07.2007

Associate of Science - Medical Laboratory Technology

HAHNEMANN UNIVERSITY

Bachelor of Arts - Biology

TEMPLE UNIVERSITY
David Hawkins