Dynamic scientist with extensive experience in forensic and research science, specializing in nucleic acid purification and analysis. Issued over 800 DNA reports and provided expert testimony in 60 court cases. Recognized for strong analytical skills and a collaborative approach, ensuring quality and accuracy in high-pressure environments.
Overview
34
34
years of professional experience
Work History
Forensic Scientist 3
OFFICE OF FORENSIC SCIENCE - PHILA. POLICE DEPT.
Philadelphia, USA
04.2008 - 03.2025
Analyzed evidence and reference materials, and issued more than 800 forensic DNA reports to provide investigative leads in various criminal investigations.
Created the workflow, managed the PPD DNA Lab's case outsourcing program, and performed more than 1,000 technical reviews of outsourced case files.
Performed more than 600 technical reviews of my peers' case files to ensure adherence to quality control and quality assurance requirements.
I testified as an expert in the field of forensic DNA analysis in approximately 60 criminal trials in the Commonwealth of Pennsylvania, and three trials in the Federal Court of the Eastern District of Pennsylvania.
Performed scheduled maintenance and performance checks for various instruments within the DNA Laboratory.
Research Technician
THOMAS JEFFERSON UNIVERSITY
Philadelphia, USA
07.1991 - 07.2007
Worked independently on multiple projects employing complex molecular biology techniques.
Assisted in grant writing and manuscript preparation.
Maintained laboratory records according to Good Laboratory Practices.
Assisted in the training of newly hired technicians and instructed/demonstrated molecular biology techniques to undergraduate and graduate students during lab rotations.
Entrusted with daily lab management and maintenance duties.
Education
Associate of Science - Medical Laboratory Technology
HAHNEMANN UNIVERSITY
PHILADELPHIA, PENNSYLVANIA
Bachelor of Arts - Biology
TEMPLE UNIVERSITY
PHILADELPHIA, PENNSYLVANIA
Skills
Nucleic acid purification (manual and automated)
Real-time qPCR
PCR (multiplex and conventional)
Capillary electrophoresis
Blotting techniques (Northern, Southern, Western)
SDS-PAGE
ChIP assay
DNase I footprinting
Plasmid construction
Immunofluorescence and microscopy
Radioisotope handling
Tissue culture
Cell transfection
Adenovirus production
Sequence analysis software
Laboratory management and maintenance
Microsoft Office Suite (Word, Excel, PowerPoint)
Genemapper IDX software
CODIS database management
Vector NTI software
DNA profiling
Technical review
Quality control
Data interpretation
Regulatory compliance
Publications
S. Piera-Velazquez, D.F. Hawkins, M.K. Whitecavage, D.C. Colter, D.G. Stokes, S.A. Jimenez, "Regulation of the human Sox9 promoter by Spl and CREB", Experimental Cell Research, 313, 2007, 1069-1079
D.C. Colter, S. Piera-Velazquez, D.F. Hawkins, M.K. Whitecavage, S.A. Jimenez, D.G. Stokes, "Regulation of the human Sox9 promoter by the CCAAT-binding factor", Matrix Biology, 24, 2005, 185-197
I.B. Coimbra, S.A. Jimenez, D.F. Hawkins, S. Piera-Valazquez, D.G. Stokes, "Hypoxia inducible factor-I alpha expression in human normal and osteoarthritic chondrocytes", Osteoarthritis and Cartilage, 12, 4, 2003, 336-345
D. Stokes, G. Liu, R. Dharmavaram, D. Hawkins, S. Piera-Velazquez, S.A. Jimenez, "Regulation of type-Il collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific phenotype in culture involves Sry-type high-mobility-group box (SOX) transcription factors", Biochemical Journal, 360, 2001, 461-470
G.R. Dodge, D.F. Hawkins, E. Boesler, L. Sakai, S.A. Jimenez, "Production of Cartilage Oligomeric Protein (COMP) by Cultured Human Dermal and Synovial Fibroblasts", Osteoarthritis and Cartilage, 6, 6, 1998, 435-440
P.J. Christner, L.D. Siracusa, D.F. Hawkins, R. McGrath, J.K. Betz, S.T. Ball, S.A. Jimenez, J. Peters, "A High-Resolution Linkage Map of the Tight Skin 2 (Tsk2) Locus: a Mouse Model for Scleroderma and Other Cutaneous Fibrotic Diseases", Mammalian Genome, 7, 8, 1996, 610-612
P.J. Christner, J. Peters, D.F. Hawkins, L.D. Siracusa, S.A. Jimenez, "The Tight Skin 2 Mouse - An Animal Model of Scleroderma Displaying Cutaneous Fibrosis and Mononuclear Cell Infiltration", Arthritis Rheumatism, 38, 12, 1995, 1791-1798
Abstracts
G.R. Dodge, D.F. Hawkins, S.A. Jimenez, "Modulation of aggrecan, MMPI, and MMP3 production by glucosamine sulfate in cultured human osteoarthritis articular chondrocytes", Arthritis Rheumatism, 42, suppl., 1999, S253
S. Grässel, M. L. Chu, R. Timple, D. Hawkins, G.R. Dodge, "Type XVI collagen is expressed in human articular chondrocytes and cultured synovial cells", American College of Rheumatology 64th Annual Scientific Meeting, San Diego, November, 1998
P.J. Christner, R. McGrath, D.F. Hawkins, L.D. Siracusa, S.A. Jimenez, "A mutation in the fibrillin gene influences al (I) procollagen mRNA levels in Tsk mouse embryonic cell lines", American College of Rheumatology 60th Annual Scientific Meeting, Orlando, October, 1996
P.J. Christner, L.D. Siracusa, D.F. Hawkins, R. McGrath, J.K. Betz, S.T. Ball, S.A. Jimenez, J. Peters, "A high-resolution linkage map of the tight skin 2 (Tsk2) locus: a mouse model for scleroderma (SSc) and other cutaneous fibrotic diseases", American College of Rheumatology, 60th Annual Scientific Meeting, Orlando, October, 1996
P.J. Christner, J. Peters, D.F. Hawkins, L.D. Siracusa, S.A. Jimenez, "Preliminary characterization of a novel tight skin mutation in the mouse: Tsk2", 14th Annual East Coast Connective Tissue Meeting, Baltimore, March, 1994
P.J. Christner, L.D. Siracusa, J. Peters, D.F. Hawkins, S.A. Jimenez, "Tsk2, A novel animal model of Scleroderma: Biochemical, Histopathological, and Genetic Characterization", American College of Rheumatology 58th Annual Scientific Meeting, Minneapolis, October, 1994
Laboratory Skills
Purification of nucleic acids
Real-time PCR
Multi-plex & Conventional PCR
Capillary electrophoresis
Northern blot analysis
Southern blot analysis
SDS-PAGE
Western blot analysis
ChIP assay
DNAse I footprinting
Plasmid construction
Immunofluorescent and fluorescent staining and microscopy
Radioisotope handling
Extensive tissue culture experience
Cell transfection and adenovirus production
Computer sequence analysis
General laboratory maintenance and management
Accomplishments
Entrusted to work on many cold case and high profile criminal investigations that lead to criminal convictions.
Timeline
Forensic Scientist 3
OFFICE OF FORENSIC SCIENCE - PHILA. POLICE DEPT.
04.2008 - 03.2025
Research Technician
THOMAS JEFFERSON UNIVERSITY
07.1991 - 07.2007
Associate of Science - Medical Laboratory Technology