Netra Mathada Kadalolli
Milford,USA
Summary
Diligent and results-driven Scientist specializing in Biotechnology, Cellular, and Molecular Biology with 8 years of research expertise. Proficient in leading research projects encompassing Cell Culture, Molecular Biology, and Genetics. Collaborative team player with excellent communication skills, adept at discussing technical evaluations, and presenting results to address specific challenges. Demonstrated ability to execute and troubleshoot various molecular biology techniques, coupled with a strong background in data analysis and interpretation. Excels in a highly collaborative research environment and seeks to contribute to multidisciplinary projects.
Overview
15
15
years of professional experience
1
1
Certification
Work History
Scientist II/III Regenerative Medicine - Research Beyond Borders
Boehringer-Ingelheim
12.2021 - Current
- Isolated primary Human, canine, and diseased cells from tissue
- Extracted RNA, and cDNA to perform gene expression study
- Extracted proteins from cell culture technique and performed Protein expression study
- Conducted Gene Expression Profiling and Heterogeneity study via Single-Cell RNA Sequencing (scRNA-seq)
- Prepared Samples and libraries for various sequencing needs and operating sequencers
- Performed immunostainings, and functional assays on cells via Imaging and data analysis
- Designed cell Proliferation Assay and cell cytotoxicity Assay using Incucyte (live cell imaging and analysis)
- Executed biochemical and molecular biology experiments such as PCR, RT-qPCR, ELISA, MSD, etc
- Implemented and troubleshooted various molecular biology techniques, including PCR, cloning, and DNA sequencing
- Expertise in cell culture techniques and handling biological samples
- Designed, planned, and executed experiments independently and efficiently analyzed the data
- Highly motivated, experienced in data analysis, interpretation of results, troubleshooting, and solving technical issues
- Have the ability and willingness to work effectively in a highly collaborative research environment.
Research Experience - Department of Cellular and Molecular Biology
University of New Haven
07.2019 - 05.2021
- Assessed Cytotoxicity of Docetaxel Chemotherapeutic Drug on MCF-7 cells by Alamar Blue Assay and Detected the cell migration by Invasion
- Delivered GFP (green fluorescence protein) expressing plasmid DNA into MCF7 cell lines by transfection using Electroporation and monitored the protein expression
- Isolated, Extracted, and Purified a single exon of the TP53 gene from Jurkal genomic DNA using a DNA purification Kit and amplified the TP53 gene using a PCR Thermal cycler
- Performed Site-directed Mutagenesis in p53 tumor suppressor gene from cloned and sequenced MDA-MB-231 Cancer cells
- Evaluated the Antiviral activity of Ribavirin using a TMEV viral vectors Model System
- Detected Rabies, Epstein Barr Virus, and Parvo viral genome by PCR from the human heart tissues, that was obtained from patients suffering from myocarditis
- Expressed Recombinant protein, evaluated the Enzyme activity for GST-DHFR-His protein using E
- Coli as Host cell.
Research Associate
ARAR Pharmaceutical Research Center
04.2012 - 03.2016
- Conducted Pre-formulation research for drug discovery and development of drug product formulae and composition
- Worked on in-vitro preclinical experiments and performed antiviral study
- Developed and validated ELISA assays for biomarker quantification, contributing to a 30% increase in assay sensitivity
- Optimized cell culture techniques for enhanced productivity and efficiency
- Developed project management skills
- Collaborated with cross-functional teams, external agencies, and networking, contributing to successful project outcomes
- Evaluated the immunomodulatory activity of the aqueous extract of Carica Papaya on Flavivirus in erythrocytes
- Developed standard protocols and procedures, SOPs, maintained proper records, technical reports, laboratory equipment's
- Carried out molecular biology tests like DNA-RNA Isolation and Purification, PCR-based assays, and Western Blot
- Resolved complex technical issues in experiments, and data analysis, ensuring accurate interpretation and reporting of results.
Lecturer - Department of Biotechnology
Basaveshwar College of Engineering
10.2008 - 06.2011
- Taught undergraduate courses like Cell Biology, Molecular Biology, Bio-Separation Techniques, and Genetics
- Mentored students in research projects, emphasizing hands-on techniques, lab equipment, troubleshooting skills, and data analysis
- Conducted independent research, contributing to the institution's scientific reputation
- Published 2 research works and findings in International Journals resulting in contributions to the scientific community.
Education
Master of Science - Cellular and Molecular Biology
University of New Haven
CT, United States01.2021
Master of Technology - Chemical Engineering
Siddaganga InsƟtute of Technology
01.2012
Bachelor of Engineering - Biotechnology
Visveswaraiah Technological University
Karnataka, India01.2008
Skills
- Cell Biology
- Molecular Biology
- Protein Biochemistry
- Nucleic acid
- Virology laboratory techniques
- Molecular nucleic acid biochemistry: Isolation, Extraction, Purification, and Quantification Assay of Cell/ DNA/ RNA/ protein/ virus Genomic DNA extraction, Chromatography, Separation and Purification techniques, PCR, RT-qPCR, multiplex PCR, digital PCR, ELISA, GLP, CRISPR cas9, gRNA, Restriction digestion, Molecular Cloning, DNA sequencing, Ligation, Gene Editing, single-cell RNA Sequencing scRNA-seq, Mutagenesis, SDS-PAGE, Agarose Gel Electrophoresis, DNA fingerprinting, Western Blot, Southern Blot, Northern Blot
- Cell culture techniques: Plant Tissue Culture, Cell culture, Media Preparation, Mammalian cell lines preparation, Culturing, Subculturing, Cell passaging, Electroporation, Transfection, Immunofluorescence, Cryopreservation, Immunostaining, Cell Staining, Bioassay, Cell-Based Assays like Cell Viability Assays, Cell proliferation assay, Cell toxicity assay, Cell invasion assay, Cytotoxicity, Apoptosis, multiple-color flow cytometry, and Incucyte
- Virology: cDNA synthesis, Viral Genome Detection, Bacterial Transformation, Electroporation, Lipofectamine, Transfection, Viral CPE Assessment, Viral titer Assay, Lentivirus, TCID50 Assay, Plaque assay, Antiviral assay
- Protein Biochemistry: Protein Extraction and Purification, Protein Expression, Protein Quantification, Protein-protein Interactions, Protein Assays, Western blot, Protein Structure analysis, Protein folding Study, Protein degradation, Bacterial Transformation, Protein induction, Enzyme Expression, purification, Enzymatic activity assay, SDS Page analysis, ELISA, MSD
- Immunology: Immunoassays, ligand binding-based cell assays
- Soft Skills: Data Analysis, Excel, Graph Pad Prism, Verbal Communication, Oral and writing, and PowerPoint presentation
Certification
- Molecular Biological Techniques in Biotechnology
- Gene Cloning Techniques
- Clinical Research & Clinical Data Management (CDM)
- Molecular Cloning & Its Application
Publications
Cardioprotective effect of Cucumis trigonus Roxb on isoproterenol-induced myocardial infarction in rats, American Journal of Pharmacology and Toxicology, 4 (2), 29-37, BS Thippeswamy, SP Thakker, S Tubachi, GA Kalyani, MK Netra, U Patil, S Desai, CC Gavimath, VP Veerapur, 2009 Screening of Antibacterial Activity of Seed Extracts of Heracleum Grande and Pimpinella Heyneana, Advances in Pharmacology and Toxicology, 11 (1), 23, NA Jadhav, CC Gavimath, S Kadapure, NH Naik, MK Netra, 2010 Evaluation of Antiamnesic Potentials of Citrus Reticulata in Mice, International Journal of Pharmacology and Biological Sciences, 4 (1), 145, CC Gavimath, S Kadapure, H Vidya, VA Kangralkar, MK Netra, 2010
Timeline
Scientist II/III Regenerative Medicine - Research Beyond Borders
Boehringer-Ingelheim
12.2021 - Current
Research Experience - Department of Cellular and Molecular Biology
University of New Haven
07.2019 - 05.2021
Research Associate
ARAR Pharmaceutical Research Center
04.2012 - 03.2016
Lecturer - Department of Biotechnology
Basaveshwar College of Engineering
10.2008 - 06.2011
Master of Science - Cellular and Molecular Biology
University of New Haven
Master of Technology - Chemical Engineering
Siddaganga InsƟtute of Technology
Bachelor of Engineering - Biotechnology
Visveswaraiah Technological University
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