Debasmita Saha

West Lafayette,Indiana

Summary

My research experience focuses on epigenetic and transcriptional regulatory networks associated with antifungal drug resistance. My academic background includes extensive training in microbiology, biochemistry, genetics, and molecular biology. I am committed to advancing our knowledge in disease prevention and drug discovery, significantly contributing to the identification of novel drug targets. Beyond my technical skills, I offer a proactive, problem-solving mindset. I excel at identifying potential issues early and developing innovative solutions to address them. I thrive in collaborative settings, bringing strong interpersonal skills and a team-oriented approach to my work.

Overview

Certification

Education

Ph.D. - Biochemistry

Purdue University

West Lafayette, IN

08.2024

Master of Science - Biochemistry

University of Calcutta

Kolkata, India

08.2024

Bachelor of Science - Microbiology

Unniversity of Calcutta

Kolkata, India

07.2015

Skills

Molecular Biology- Gene-editing, cloning and expression vectors, DNA/RNA nucleic acid extractions, PCR, CRISPR/Cas knock-in/ knockout, gel electrophoresis, site-directed mutagenesis, qPCR, western blot, SDS-PAGE, primer design

Biochemical- Protein expression in bacterial system and purification - ion exchange, Affinity Chromatography, Gel filtration, Size Exclusion Chromatography (SEC), western blot, SDS-PAGE, Spectrophotometry, Protein-DNA interaction using Chromatin Immunoprecipitation (ChIP), auxin-induced protein degradation (similar to PROTAC)

Cell Biology- Subcellular fractionation of bacterial, yeast and mammalian cells, cell-based assays (cell viability assay, adhesion assay, fluorescent based assays, biofilm formation assay), minimal inhibitory concentration (MIC), microbial growth curve, transformation, electroporation, transfection in bacterial and yeast cells

Microbiology - Aseptic techniques of handling bacteria and fungi under aerobic and anaerobic/hypoxic conditions, culturing, inoculation, identifying different classes of microorganisms, staining techniques

In vivo host model experience- Handling and injection of wax moth larvae Galleria mellonella as

infection model organism with Candida glabrata clinical isolates with and without azole antifungal drug treatment

Computation skills: Microsoft office (Word, Excel, PowerPoint), sequence analysis using EMBOSS, CLUSTAL omega, BLAST, MAFFT sequence alignment, MEGA and fasttree to build protein and species phylogenetic tree, Jupyter notebook, Snapgene, Biorender, Graphpad Prism, Image J, Unix, R (intermediate level)

Time Management
Attention to Detail
Flexible and Adaptable
Dependable and Responsible

Certification

● Good Clinical Practice (GCP) training certificate awarded by NIDA CCTN CTN (2023).

● Principles of Good Pharmacology training (PK/PD, clinical study design, drug development, ADMET), NIH Office of Clinical Research (2023).

● Principles and Practice of Clinical research training, offered by NIH Office of Clinical Research, offered by NIH Office of Clinical Research (2023).

Timeline