Debra Rollo
Houston
Summary
Diligent Clinical Laboratory Technologist with proven track record of accurate and timely diagnostic testing. Demonstrated ability to manage laboratory operations and maintain high standards of quality control. Proficient in using advanced laboratory equipment and effective in troubleshooting technical issues.
Overview
20
20
years of professional experience
1
1
Certification
Work History
Clinical Laboratory Technologist III
NeoGenomics Laboratories
10.2019 - Current
- DNA / RNA extractions
- FFPE, Whole Blood, and Tissue
- Qiagen DNA, RNeasy, All Prep, etc
- HPV genotyping - Fragment Analysis
- Real-Time PCR for qualitative assessment of FGF 19 expression
- Genotyping studies - Sanger Sequencing
- Library preparation and enrichment - DNA and RNA
- Proficient with Illumina sequencers- HiSeq, MiSeq, NovaSeq, NextSeq
- Liquid Biopsy (Ion Torrent/S5) and Nanostring
Research Associate- Microarray Supervisor / Lab Technologist - Research Associate/NGS
Family Tree DNA
09.2017 - 10.2019
- High throughput DNA extraction and Amplification
- High throughput genome-wide association studies using Illumina SNP-based bead array chip technology
- Experience in both automated and manual prep
- High throughput library preparation utilizing Nextera Rapid and Qiagen QFX kits and Next-Generation Sequencing using Illumina Novaseq technology
- Supervise and manage every day flow of Microarray pipeline- team size 4-8
Laboratory Coordinator
University of St. Thomas
06.2015 - 10.2017
- Oversee all aspects of undergraduate Biology labs including; organization, set-ups, ordering supplies and equipment, budgets, hazardous waste disposal
- Prepare solutions, specimens, prokaryotic and eukaryotic cell culture, gel preparation
- Hire, supervise, and manage 10-20 undergraduate teaching assistants
- Mentor undergraduate research
Microarray Technologist
Family Tree DNA
08.2013 - 06.2015
- Conducting high throughput genome-wide association studied using SNP-based bead array chip technology (Illumina/Iscan)
Research Assistant II
University of Texas Health and Science Center
01.2010 - 01.2013
- Real-time data collection, as dictated by the study guidelines, of acute trauma patients as they receive prescribed care
- Enrollment and subsequent tracking of patients from ER to OR and Acute care setting
- Data entry and maintaining patient record for duration of patient stay
Transfer Coordinator
Memorial Hermann Healthcare Systems
01.2005 - 01.2010
- Coordination of all patient hospital to hospital transfers into and out of the Memorial Hermann System
- Facilitated communication between Physicians in order to help patients receive the best care options.
Education
B.S - Molecular Genetic Technology
University of Texas
2013
Skills
- DNA sequencing
- Microbiology techniques
- Laboratory information systems
- Molecular diagnostics
- Instrument maintenance
- Laboratory equipment operation
- Scientific research
- Lab test validation
- Sample preparation
- Laboratory safety protocols
- Staff management
- Instrument Calibration
- Laboratory documentation
- Critical thinking
- Good laboratory practices (GLP)
- Decision-making
Certification
ASCP- Molecular Biology 2013-Current
Publication
ArunKumar G, Tatarinova TV, Duty J, Rollo D, Syama A, Arun VS, Kavitha VJ, Triska P, Greenspan B, Wells RS, et al. Genome-wide signatures of male-mediated migration shaping the Indian gene pool. J Hum Genet. 2015 May 21. doi: 10.1038/jhg.2015.51. PubMed PMID: 25994871.
Timeline
Clinical Laboratory Technologist III
NeoGenomics Laboratories
10.2019 - Current
Research Associate- Microarray Supervisor / Lab Technologist - Research Associate/NGS
Family Tree DNA
09.2017 - 10.2019
Laboratory Coordinator
University of St. Thomas
06.2015 - 10.2017
Microarray Technologist
Family Tree DNA
08.2013 - 06.2015
Research Assistant II
University of Texas Health and Science Center
01.2010 - 01.2013
Transfer Coordinator
Memorial Hermann Healthcare Systems
01.2005 - 01.2010
B.S - Molecular Genetic Technology
University of Texas
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