Summary
Overview
Work History
Education
Skills
Training
PATENT
Publications
Awards
Certification
Timeline
Generic

Deepali Lehri

Elmsford,NY

Summary

My research focuses primarily focuses on bioinformatics-driven genomics, where I contributing in designing and implementing of advanced workflows for Single-cell seq, RNA-seq, Whole genome seq, and cellfreeDNA analysis using tools such as Seurat and clusterProfiler. I led bioinformatics-driven genomics projects with direct public health imapct, most notably contributing to New York State's COVID-19 surveillance program. I am working on building and curating genome databases for rabbit and chicken. My expertise lies at intersection of big data analysis, bioinformatics workflows and applied genomics, complemented by experience in molecular and applied genomics, complemented by experience in cell and molecular biology techniques that will provide context for data interpretation. During my research in PhD, I worked with R&D and other departments of edible oil industry (BL.Agro Industries ltd., India), resulting in publications, a patent (in progress) and industry collaborations.

Overview

4
4
years of professional experience
1
1
Certification

Work History

Post Doctoral Research Fellow

New York Medical College
01.2023 - Current

Genomics Core, Department of Pathology, Microbiology and Immunology

  • Conducting COVID-19 variant sequencing for NY State public health surveillance program with Department of Health
  • Executing RNA-seq, mRNA-seq, single-cell RNA-seq, WGS, and cfDNA analysis
  • Sequencing libraries constructionusing 10X, PARSE, Illumina and Genexus platforms
  • Mitochondrial and nuclear DNA extraction, QC, and annotation
  • Building curated genome databases for rabbit and chicken
  • Implementing bioinformatics workflows: Scanpy and clusterProfiler

Research Associate

University Of Nebraska Med Ctr
02.2022 - 01.2023

Dept. of Ophthalmology and Visual Sciences

  • Understanding and investigating the initiation and progression of age related pathologies (cataract, glaucoma and macular degeneration) in eyes
  • Conducted lens epithelial and cell culture experiments
  • Authored and co-authored peer-reviewed articles

Education

Ph. D - Biotechnology

Banasthali Vidyapith
10.2021

M. Sc. - Biotechnology

Banasthali Vidyapith
07.2017

B. Sc. - Biotechnology

Banasthali Vidyapith
06.2015

Skills

  • Sequencing & Genomics: RNA-seq, mRNA-seq, scRNA-seq (10X, Parse, Seqwell), WGS, cfDNA extraction, COVID-19 variant analysis and SARS-CoV-2 sequencing workflows
  • Library Construction & QC: Illumina, Genexus, FastQC, Bioanalyzer, NanoDrop, Tapestation, BluePippin
  • Genome & Database Work
  • Genome annotation (rabbit, chicken), sequence curation, species-specific DB development
  • Bioinformatics & Analysis: featureCounts, DESeq2, edgeR, Seurat, clusterProfiler, UCS Genome Browser, Linux CLI, R, Python (basic)
  • Molecular Biology & Cell Techniques: qPCR, RT-PCR, CRISPR, DNA/RNA extraction, cloning, Western blotting, SDS-PAGE, CRISPR-Cas9
  • Cell culture: Maintenance of mouse lens epithelial cell lines, human lens epithelial cell lines, subculturing adherent cell lines, freezing and thawing cell lines, cell counting, viability assays, confocal and fluorescence microscopy
  • Nanotechnology: Nanoemulsion design, FTIR, ZetasizerInvestigation of properties of amphiphilic properties, nanoemulsions preparation, enzymatic modification of bioactive lipids, value added products from by- products obtained during edible oil refining, lipid nanotechnology, FTIR, Zeta sizer analysis
  • Writing and communication: Experience in writing scientific manuscripts, drafting SOPs
  • Laboratory Management: Managing people on the research team for various research projects, maintaining laboratory notebooks, drafting and finalizing SOPs, facilitated laboratory meetings, managing data collection, analysis and final report writing

Training

  • “Training on cell culture and molecular techniques” from Council of Scientific & Industrial Research- Central Drug Research Institute, Lucknow-Uttar Pradesh, India
  • “Techniques related to molecular cloning and CRISPR technology of gene editing” from Council of Scientific & Industrial Research-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow-Uttar Pradesh, India
  • “Basic Techniques in Plant Biotechnology” from Council of Scientific & Industrial Research-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow-Uttar Pradesh, India
  • “Basic techniques in biotechnology” from Council of Scientific & Industrial Research-National Botanical Research Institute (CSIR-NBRI), Lucknow-Uttar Pradesh, India

PATENT

Filed an Indian patent “A Novel Process and Kit for Extraction of DNA”

Publications

  • Chhunchha B, Ramalingam Manoharan R, Lehri D, Kubo E, Singh DP. Prdx6 Inhibits Oxidative Stress-Driven Txnip-Nlrp3 Inflammasome Activation And Mitigates Pyroptosis In Lens Epithelial Cells. Investigative Ophthalmology & Visual Science, 2025.
  • Chhunchha B, Kubo E, Lehri D, Singh DP. NLRP3 Inflammasome and Inflammatory Response in Aging Disorders: The Entanglement of Redox Modulation in Different Outcomes. Cells, 2025.
  • Martz J, Hammer B, Langen TJ, Berkowitz B, Storm JA, Lu J, Lehri D, Wijenayake S, Marrocco J Kentner AC. Investigating milk-derived extracellular vesicles as mediators of maternal stress and environmental intervention. bioRxiv,2025.https://doi.org/10.1101/2025.05.30.566911
  • Lehri D, Kumari N, Singh RP. Ultrasound-assisted production and characterization of rice bran lecithin-based nanoemulsions. Journal of Dispersion Science and Technology, 2021. 42(9):1368–1375.
  • Lehri D, Kumari N, Singh RP, Sharma V. Composition and applications of rice-derived lecithin – A review. Plant Science Today, 2019. 6(sp1):613–622.
  • Lehri D, Kumari N, Singh RP. Production of cocoa butter equivalents using palm mid fraction and kokum butter. Journal of Lipid Science and Technology, 2020. 52:77–86.

Awards

  • Prime Minister’s Fellowship Scheme for Doctoral Research by Department of Science and Technology-Science and Engineering Research Board, Government of India and Confederation of Indian Industry (DST-SERB and CII).
  • DBT-Scholarship for Biotechnology during postgraduation by Department of Biotechnology (DBT), Government of India DBT funded Project training by the Department of Biotechnology, Government of India for dissertation during postgraduation in Banasthali Vidyapith, Rajasthan-India.
  • Second position in Scientific Model Making, in Janus-2014, Faculty of Life Sciences, Banasthali Vidyapith, Rajasthan-India.
  • Best Poster Presentation, PMI-3rd Annual Symposium, New York Medical College, Valhalla, New York.

Certification

Skill Development Program for Postgraduate Students at CSIR-Central Drug Research Institute, Lucknow Details of Training: Training on cell culture and molecular techniques Duration of Course: 2021-11-24 to 2021-12-20

Timeline

Post Doctoral Research Fellow

New York Medical College
01.2023 - Current

Research Associate

University Of Nebraska Med Ctr
02.2022 - 01.2023

B. Sc. - Biotechnology

Banasthali Vidyapith

Ph. D - Biotechnology

Banasthali Vidyapith

M. Sc. - Biotechnology

Banasthali Vidyapith
Deepali Lehri