DIWAKER TRIPATHI
Maple Valley,WA
Summary
Detail-oriented team player with strong organizational skills. Ability to handle multiple projects simultaneously with a high degree of accuracy. Adaptable and hardworking individual with excellent problem-solving skills. Resourceful and adaptable individual with extensive experience developing and conducting experiments to document results for studies. Comfortable handling complex issues, meeting strict deadlines and adjusting to rapidly changing conditions.
Overview
9
9
years of professional experience
1
1
Certification
Work History
Acting Instructor
University of Washington
05.2017 - Current
- Investigating properties and functions of the organelle DNA and how DNA properties change during development
- Using molecular tools such as ELISA, PCR, qPCR, and Next-Gen-Sequencing (NGS) to address the research problems
- Currently using AWS cloud technology for analyzing sequencing data
- Managing the lab and designing/setting up the experiments
- Collecting the data and performing data analysis and preparing manuscripts for publication
- Contributing towards research grant as Co-PI and collaborating with other labs
- Supervising and mentoring the lab staff and undergraduate students.
Postdoctoral Researcher
Washington State University
07.2014 - 05.2017
- Investigated the molecular mechanism of extracellular ATP induced defense against pathogens; designed molecular assays for pathogen detection
- Contributed to winning the grant (NSF-IOS 1529746; amount $750,000) for the adviser and published three research articles as lead authors
- Worked well independently and on a team in collaboration with other labs/institutions to solve research problems to solve research problems.
Education
Ph.D - Molecular Sciences
Washington State University
Pullman, WAM.S - Biology/Biochemistry
East Tennessee State University
M.Sc./M.S - Biotechnology
Barkatullah University
B.Sc./Bachelor’s - Zoology, Botany, and Chemistry
University of Lucknow
Skills
- Molecular biology - Isolation/extraction and characterization of nucleic acids (DNA, RNA) and proteins
- Genetic engineering- molecular cloning (primer/probe designing, cDNA cloning, expression construct design/generation including RNAi, siRNA, shRNA), RNA structure/function, RNA silencing, and virus-induced gene silencing (VIGS), gene editing (CRISPR/CAS9), gene delivery, nucleic acid detection techniques (Northern, Southern, UPLC-MS, DSC, DLS), cell manipulation- transduction and transfection
- Genomics - Next-generation sequencing (NGS), DNA/RNA/cDNA, ScRNA and RNA-seq, WGS, Fragment libraries, MethylSeq, Targeted sequencing, Single-cell genomics
- Biophysical Studies- Flow cytometry, calorimetry, CD, Fluorescence, IR, UV Spectrophotometer, PCR, real-time or qPCR, ddPCR, ultracentrifugation, Mass spec
- Immunology- ELISA (DAS, DAC), ELISPOT, FACS, Luminex, Pathogen assays, SPR immunoassay, antibody engineering
- Protein Biochemistry- Protein folding, assembly and trafficking processes, Protein expression, characterization, purification, interaction assays, X-ray protein crystallography, Protein modeling, Chromatography
- Microbiology - Small and large-scale mammalian cell culture, Aseptic procedures, Stem cells (iPSC) or (HSC) generation, Human T and B cells
- Cell biology and Microscopy - Fluorescence, Luminescence, Electron, Confocal microscopy, MHC multimer staining, Intracellular cytokine staining (ICS), Cell-based assays (cell viability, proliferation, senescence, toxicity, death)
- Computer Skills
- Programming language (Sql/C/C/Python/R)
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
- Cloud computing technologies
- Microsoft Office (Word, Outlook, Excel, Access, PowerPoint, Publisher, Linux, XP) Coral draw, and Adobe Photoshop
- Biostatistical (SAS, JMP, GIS, Spotfire) and bioinformatics software (Structural and functional analysis of biomolecules (such as Vector NTI; iPlant tools, ImageJ), Use of public data repositories (ENCODE, TCGA, Ensembl, NCBI, GitHub, BaseSpace, Sequencher), Electronic Laboratory Notebooks (ELN)
- General lab practices; Good lab practices;
- Radiation safety; Intellectual Property Rights; Quality Control Parameter - Microbiological Analysis & IPR Proceedings; Immunology
- Planning and Coordination
- PPE Compliance
- Organization and Time Management
- Attention to Detail
- Self-Motivated
- Teamwork and Collaboration
- Excellent Communication
- Decision-Making
- MS Office
Accomplishments
- Graduate studies outstanding thesis award, 2011
- Global Plant Science Initiative (GPSI) fellowship at WSU, 2010-14
- Fraley Memorial Scholarship for Graduate Research, 2009
Certification
Data Science - Python and R Statistics for data science; Data Science using R and Python; Apache Spark and Scala; A.I. & Deep Learning with TensorFlow; Data visualization (Tableau) (2020 -).
Bioinformatics - Genomic Data Science and Clustering (Bioinformatics V); Introduction to Genomic Technologies; Biology Meets Programming; Bioinformatics for Beginners; Comparing Genes, Proteins, and Genomes (Bioinformatics III); Genome Sequencing (Bioinformatics II); Finding Hidden Messages in DNA (Bioinformatics I) (2018-19).
Affiliations
Associate Editor and reviewer for several journals, 2017-2022
Ambassador (American Society of Plant Biologist), 2017-2022
Member of Sigma Xi - Research Honor Society, American Society of Plant Biologist (ASPB), and American Association for the Advancement of Science (AAAS), ETSU.
Timeline
Acting Instructor
University of Washington
05.2017 - Current
Postdoctoral Researcher
Washington State University
07.2014 - 05.2017
Ph.D - Molecular Sciences
Washington State University
M.S - Biology/Biochemistry
East Tennessee State University
M.Sc./M.S - Biotechnology
Barkatullah University
B.Sc./Bachelor’s - Zoology, Botany, and Chemistry
University of Lucknow
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