Doha Sleem

• Attend Biol 150 principal of molecular and cell biology lectures, lead discussion with students, .
• Check assignments, proctor tests and provided grades according to university standards.
• Mentored students through office hours and one-on-one communication.

Write a review on gamma-secretase, an intramembrane cleaving protease and its role in Alzheimer's disease, as well as the use of chemical probes to study this protease enzyme.

Goal: understand how both biochemistry and genetics contribute to Alzheimer's disease by altering the structure of gamma-secretase and the utility of medicinal chemistry approaches to develop therapeutic techniques that target this protease complex.

• Attended online summer Biol 400 that offered fundamentals of Microbiology.
• Checked discussion board participation, which was in the form of weekly online discussion board questions, and provided grades according to university standards.

Investigate the impact of defects in iron trafficking and storage on transposon silencing and C. elegans is used as a model for this study.

Goal:

• Conducted genetic crosses and phenotypic assays under varying iron conditions, including low and high iron environments, as well as feeding the animals with iron-deficient bacteria.
• Performed liquid culture assays to create a controlled media environment.
• Explored the role of the HAF-6 ABC transporter through biochemical assays such as protein expression and purification, as well as ATP assays.

• Taught Biol 154, a lab which requires the students to acquire scientific skills including critical thinking,
  quantitative reasoning, scientific communication, and collaboration.
• Taught the students experimental design ranging from cells to ecosystems, evolution, genetics,
  and physiology.
• Checked assignments and provided grades according to university standards.
• Mentored students through office hours and one-on-one communication.
• Documented attendance and completed assignments to maintain full class and student records.

Investigating the function of SUMOylated proteins during mitosis and their interplay with Topoisomerase II to ensure accurate chromosomal segregation in the M phase. This study involves tasks such as cell culture, immunostaining, and sub-cloning to comprehensively explore this relationship.

• Taught Biol 247 and 547 by using experiments and simulations to demonstrate laboratory techniques and representative processes in areas of human physiology.
• Checked assignments and provided grades according to university standards.
• Mentored students through office hours and one-on-one communication.
• Documented attendance and completed assignments to maintain full class and student records.

Examining the subcellular localization of beta-catenin in HCT116betaM cell lines to investigate its involvement in Adenomatous Polyposis Coli (APC). This research involves the implementation of mammalian tissue culture techniques and dual luciferase assays to gain insights into the mechanisms underlying this phenomenon.

Exploring the molecular and biophysical architecture of Gram-negative type 3 secretion system to investigate the structure of its key components, including needle, tip, and translocon proteins. This investigation requires employing biochemical assays, particularly focusing on the expression and purification of translocon proteins, aims to unearth potential antibiotics effective against antibiotic-resistant genes prevalent in Gram-negative bacterial strains.

• Taught science, pharmacy and pre-med students principals of molecular and cell biology; fundamental of microbiology; introduction of biochemistry; principals of organic and general chemistry.
• Designed science dictionary and flash cards with chemistry and biological sciences terminology.
• Assisted students in developing study skills and strategies.
• Motivated students with accurate feedback and positive reinforcement.
• Assessed student learning needs and provided relevant instruction to close knowledge gaps.

Undertaking ecotoxicological and biological field investigations on the saw-scaled viper (Echis pyramidum) and Kenyan sand boa (Eryx colubrinus) residing in the El-Faiyum desert, Egypt. This project involves the analysis of heavy metals, along with biochemical and histological assays to comprehensively evaluate their ecological and physiological status.

The project's objective is to assess the impact of heavy metal toxicity on Nile Tilapia (Oreochromis niloticus) inhabiting the Nile River in Cairo, Egypt. This entails quantifying the concentrations of heavy metals in various biological and environmental samples, including liver, kidney, muscle tissues, as well as water and sediment samples.