Donavan Bridges
Baltimore,Maryland
Summary
Experienced and dependable research engineer with a proven track record of efficiently completing tasks in various settings. Skilled in equipment operation and maintaining a clean and organized workspace. Knowledgeable in a variety of research techniques applicable to chemical engineering. Safety-conscious with a strong work ethic and the ability to adapt to different environments. Ready to contribute to a dynamic team and make a positive impact.
Overview
1
1
year of professional experience
Work History
Research Technician
Chua Lab, Johns Hopkins School of Medicine, Institute of Cellular Engineering: Neuroregeneration
Baltimore, Maryland
05.2023 - Current
- Investigated ferritinophagy in BPAN using human neurons differentiated from human induced pluripotent stem cells, and how autophagic disorders such as Alzheimer's and Parkinson's are affected
- Executed cell culture of human induced pluripotent stem cell differentiation into astrocytes, muscle, neurons
- Utilized immunocytochemistry to localize NCoA4 within wild type and BPAN neurons
- Conducted western blots measuring relative amounts of ferritinophagy-related proteins NCoA4 and FTH1.
Research Technician
Oliver Lab, Johns Hopkins Bayview Medical Center, Department of Allergy and Asthma
Baltimore, Maryland
05.2023 - 08.2023
- Examined the relation of eczema to the prevalence of allergic allergy disorder in skin, specifically keratinocytes, and the presence of PGD2 in late-phase allergic response
- Conducted experiments on skin biopsies, measuring the allergy-related chemical release in response to different treatments.
Education
Bachelor of Science - Chemical and Biomolecular Engineering
Johns Hopkins University
Baltimore, Maryland05.2024
Skills
- MATLAB
- Python
- AutoCAD
- Aspen
- PyRosetta
- Cell Culture (iPSCs, muscle, astrocytes, neurons)
- Experimental design
- Establishing procedures
- Laboratory instrumentation
- Conducting experiments
- Western blotting
- Immunocytochemistry
Projects and Presentations
- Poster: Investigating Impairment of Ferritinophagy in BPAN, Towson, Maryland, Falling for Research Conference, Towson University, 10/2023, Presented summer research focusing on the status of ferritinophagy in Beta-Propeller Protein-Associated Neurodegeneration (BPAN) focusing on NCoA4 and FTH1
- Mechanical Engineering Freshman Lab II, Baltimore, Maryland, Filmed a short story movie using Arduino coding and motors, 01/2021 - 04/2021
- Individual Portion of Design Project, Baltimore, Maryland, Coded and built a diorama with two motors, a button, and lights
- Mechanical Engineering Freshman Lab I, Baltimore, Maryland, Designed a device using multiple simple machines to move an egg two inches vertically and six inches horizontally without the egg cracking., 08/2020 - 12/2020
Publications
Skin explants: An ex vivo method for the study of skin mast cell activation, Barksdale, K., Villanueva, C. R., Owolabi, T., Bridges, D., Chichester, K., Saini, S., Oliver, E., Journal of Allergy and Clinical Immunology, 153, 2, 2024, https://doi.org/10.1016/j.jaci.2023.11.727
Languages
English
Native/ Bilingual
Spanish
Professional
Russian
Elementary
Websites
Timeline
Research Technician
Chua Lab, Johns Hopkins School of Medicine, Institute of Cellular Engineering: Neuroregeneration
05.2023 - Current
Research Technician
Oliver Lab, Johns Hopkins Bayview Medical Center, Department of Allergy and Asthma
05.2023 - 08.2023
Bachelor of Science - Chemical and Biomolecular Engineering
Johns Hopkins University
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